Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,553,840 C→T 6.9% I169I (ATC→ATT)  PP_3138 → VirK domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,553,8400CT 6.9% 169.4 / 2.2 58I169I (ATC→ATT) PP_3138VirK domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (25/29);  new base T (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

TGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGT  >  NC_002947/3553692‑3553983
                                                                                                                                                     |                                                                                                                                               
tGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATc                                                                                                                                                 >  6:231255/1‑149 (MQ=255)
  gTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCca                                                                                                                                               <  3:95956/149‑1 (MQ=255)
        gACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAg                                                                                                                                          <  7:213021/148‑1 (MQ=255)
             gCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATc                                                                                                                                                 >  3:226472/1‑136 (MQ=255)
             gCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATc                                                                                                                                     >  7:161275/1‑148 (MQ=255)
                  tCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGc                                                                                                                               >  2:250210/1‑149 (MQ=255)
                         cAAGCGCGAAGGCGAGCTGGTGCTGAACCAGTTCCAGTCCGACCTGCGTGTGGCATACCTTGCCTTCACCATGGGCCGCAG‑CCAGGGTGAAACCTGCCTGATCATTGGTGCGGTGCACGGCATCAAAAAAGGTATCCACAGCTAAACCt                                                                                                                        >  1:243212/1‑149 (MQ=255)
                          aaGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTc                                                                                                                       >  1:162565/1‑149 (MQ=255)
                           aGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCg                                                                                                                      >  4:32066/1‑149 (MQ=255)
                            gcgcGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCATGGCATCCACAAAGGTATCGACAGCGAAACCTCGc                                                                                                                     >  6:121216/1‑149 (MQ=255)
                              gcgAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTg                                                                                                                   <  1:144972/149‑1 (MQ=255)
                                  aGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCa                                                                                                               >  1:133178/1‑149 (MQ=255)
                                       aGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTAc                                                                                                          <  2:247542/149‑1 (MQ=255)
                                            gTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtga                                                                                                     >  5:270137/1‑149 (MQ=255)
                                            gTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtga                                                                                                     <  7:21609/149‑1 (MQ=255)
                                                tGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacct                                                                                                  >  2:134678/1‑148 (MQ=255)
                                                  aaCCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCATGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacctga                                                                                                <  5:121216/148‑1 (MQ=255)
                                                  aaCCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacctgac                                                                                               >  2:101020/1‑149 (MQ=255)
                                                     cTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGtgacatgacc                                                                                              >  3:230599/1‑147 (MQ=255)
                                                        ttCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGATCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAg                                                                                           <  1:101020/147‑1 (MQ=255)
                                                           cAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACtt                                                                                      >  7:258358/1‑149 (MQ=255)
                                                                    cTGCGTGTGGCATCCCTTGCCTTCTCCCTGGGCCGCAG‑ACAGGGTTAAACCAGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCTAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGc                                                                              <  3:302302/148‑1 (MQ=255)
                                                                    cTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCt                                                                             >  7:261266/1‑149 (MQ=255)
                                                                               aTCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACg                                                                    >  3:182045/1‑147 (MQ=255)
                                                                               aTCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACg                                                                    >  6:213590/1‑147 (MQ=255)
                                                                                 cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                                                                 <  6:206715/132‑1 (MQ=255)
                                                                                 cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                                                                 <  6:202023/132‑1 (MQ=255)
                                                                                 cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                                                                 >  5:206715/1‑132 (MQ=255)
                                                                                 cccTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATTCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAg                                                                                 >  5:202023/1‑132 (MQ=255)
                                                                                   gcaggtttCACCCT‑GGCTGCGGCCCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCt                                                              >  7:65166/7‑149 (MQ=255)
                                                                                   cTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCt                                                              <  4:182045/149‑1 (MQ=255)
                                                                                      gCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTg                                                             <  8:280482/147‑1 (MQ=255)
                                                                                        cTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGc                                                         >  2:278827/1‑149 (MQ=255)
                                                                                         ttCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGc                                                                <  3:215023/141‑1 (MQ=255)
                                                                                         ttCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGc                                                                >  4:215023/1‑141 (MQ=255)
                                                                                           cACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTc                                                       <  2:265446/148‑1 (MQ=255)
                                                                                                  ggCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGt                                                <  8:261266/148‑1 (MQ=255)
                                                                                                     cGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGaa                                            <  3:32066/149‑1 (MQ=255)
                                                                                                         g‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGc                                        <  8:258358/149‑1 (MQ=255)
                                                                                                             aGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTg                                     <  1:134678/149‑1 (MQ=255)
                                                                                                              gggTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTgg                                    <  8:65166/149‑1 (MQ=255)
                                                                                                               ggTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTggg                                   <  1:250210/149‑1 (MQ=255)
                                                                                                                 tGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCa                                 <  5:231255/149‑1 (MQ=255)
                                                                                                                  gAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGaa                                                    >  2:146011/1‑129 (MQ=255)
                                                                                                                  gAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGaa                                                    <  1:146011/129‑1 (MQ=255)
                                                                                                                     aCCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGaa                              >  1:92051/1‑148 (MQ=255)
                                                                                                                       ctgcctgTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCt                           >  2:130564/1‑149 (MQ=255)
                                                                                                                        tgcctgTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCtt                          >  3:177332/1‑149 (MQ=255)
                                                                                                                          cctgTTCATTGGTGCGGTGCATGGCATCCACAAAGGTATGGACAGCGAAACCTCGCTGGCCATCTTCGGTGAGCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCATCGTGTTGCTCGAAGCGTTGAATTGCCTGGGCATAACCtt                          <  2:243212/147‑1 (MQ=255)
                                                                                                                          cctgTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCtt                          <  1:130564/147‑1 (MQ=255)
                                                                                                                          cctgTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTgg                        <  8:161275/149‑1 (MQ=255)
                                                                                                                          cctgTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTg                         <  2:162565/148‑1 (MQ=255)
                                                                                                                              ttCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGgt                      <  4:177332/147‑1 (MQ=255)
                                                                                                                                      tgcggtgcAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGc                                        >  7:172516/1‑121 (MQ=255)
                                                                                                                                      tgcggtgcAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGc                                        <  8:172516/121‑1 (MQ=255)
                                                                                                                                        cggtgcAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTggg                                   >  4:55134/1‑124 (MQ=255)
                                                                                                                                        cggtgcAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTggg                                   <  3:55134/124‑1 (MQ=255)
                                                                                                                                             cAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGc     >  7:77440/1‑149 (MQ=255)
                                                                                                                                               gggCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCg   <  1:278827/149‑1 (MQ=255)
                                                                                                                                                ggCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGt  <  6:270137/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
TGGTGCTGGACCGGCCAATCTGGTTCAAGCGCGAAGGCGAGCTGGTGCTGAACCTGTTCCAGGCCGACCTGCGTGTGGCATCCCTTGCCTTCACCCTGGGCCGCAG‑CCAGGGTGAAACCTGCCTGTTCATTGGTGCGGTGCAGGGCATCCACAAAGGTATCGACAGCGAAACCTCGCTGGCCATCTACCGTGACCTGACCAAGGACTTCGAAGGGCTGCGGCCACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGT  >  NC_002947/3553692‑3553983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑