Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,558,479 C→T 8.0% R260C (CGT→TGT)  PP_3142 → sugar transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,558,4790CT 8.0% 139.6 / 2.3 50R260C (CGT→TGT) PP_3142sugar transferase
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base T (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTGCTGGCTGTTGCGCTG  >  NC_002947/3558331‑3558622
                                                                                                                                                    |                                                                                                                                               
ccGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAgcgtgc                                                                                                                                                 >  7:65362/1‑149 (MQ=255)
        gcatTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGaaa                                                                                                                                           <  8:65362/147‑1 (MQ=255)
          atTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGc                                                                                                                                       >  8:253227/1‑149 (MQ=255)
                        ccTGGTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCg                                                                                                                                     <  5:308623/137‑1 (MQ=255)
                        ccTGGTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCg                                                                                                                                     >  6:308623/1‑137 (MQ=255)
                          tGGTGACGCCGCTGGGGGGCTCCGAAGTAATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATATTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCAGGCATTCCGGTGCt                                                                                                                        >  3:87961/1‑148 (MQ=255)
                            gTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGAc                                                                                                                     <  7:253227/149‑1 (MQ=255)
                                 gccgcTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAgcgtgcgt                                                                                                                                               >  4:192399/1‑118 (MQ=255)
                                 gccgcTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAgcgtgcgt                                                                                                                                               <  3:192399/118‑1 (MQ=255)
                                 gccgcTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCt                                                                                                                        <  1:76573/141‑1 (MQ=255)
                                 gccgcTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCt                                                                                                                        >  2:76573/1‑141 (MQ=255)
                                 gccgcTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGt                                                                                                                >  2:82679/1‑149 (MQ=255)
                                              tCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCtt                                                                                                   >  1:151426/1‑149 (MQ=255)
                                              tCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCtt                                                                                                   >  2:252636/1‑149 (MQ=255)
                                                       atcaACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCAt                                                                                          >  1:229781/1‑149 (MQ=255)
                                                       atcaACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCAt                                                                                          >  6:23593/1‑149 (MQ=255)
                                                         caactttgttTACTTCAAATTCCTCTACAAGTGCATTGCGGTCAATTGGGTGCCGGACATTTTGACTTTACCCCTTATCAACCATAGCGTGCTTCAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGa                                                                                        <  1:82679/143‑1 (MQ=255)
                                                           cgatcttgtACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCATCCATAGCGTGCGTGAAATTGCCGGCATTCCCGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGc                                                                                      <  7:213399/143‑1 (MQ=255)
                                                           aCGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGc                                                                                      <  5:23593/149‑1 (MQ=255)
                                                               tgtgTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGt                                                                                  >  4:173535/1‑149 (MQ=255)
                                                                 tgtACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTc                                                                                <  3:173535/149‑1 (MQ=255)
                                                                  gtACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAACCTGTTCt                                                                               >  7:68653/1‑149 (MQ=255)
                                                                  gtACATCAAATTGCTCGACAAGTGCATTGCGGACAAATGCGACCCCGACATCTACACGTTACGCCTGATCAACCATAGCGTGCGTGAAATTCCCCGCATTCCGCTGCTTACCTTGACGCAAACACCCTTGACGGCCATGAGCCAGATCt                                                                               >  8:213399/1‑149 (MQ=255)
                                                                     cTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGTTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTg                                                                             >  8:33249/1‑148 (MQ=255)
                                                                         aaaTTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGaaaaa                                                                        >  3:255463/1‑149 (MQ=255)
                                                                            ttGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCt                                                                     <  2:229781/149‑1 (MQ=255)
                                                                             tGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGTGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACggg                                                                                             <  1:85485/124‑1 (MQ=255)
                                                                             tGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGTGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACggg                                                                                             >  2:85485/1‑124 (MQ=255)
                                                                              gCTCGACATGTGCATTGCGTTCAATTGGTTTCCCGACATCTTCTCGTTTCGCCTGATCAACCATAGCGTGCGTGAATTTGCTGTCATTCCGGTGCTGACCTTGTCGGAAACACGATTGACGGGCATGAGCCGGTTCTTGAAAAACCtt                                                                    <  4:87961/148‑1 (MQ=255)
                                                                                tCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTg                                                                   >  5:325208/1‑147 (MQ=255)
                                                                                tCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTg                                                                   >  7:112824/1‑147 (MQ=255)
                                                                                  gACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTg                                                                   <  5:26489/145‑1 (MQ=255)
                                                                                  gACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTg                                                                   >  6:26489/1‑145 (MQ=255)
                                                                                      aGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCgg                                                           <  2:151426/149‑1 (MQ=255)
                                                                                        tGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGt                                                         >  2:287916/1‑149 (MQ=255)
                                                                                          cATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTg                                                        <  4:255463/148‑1 (MQ=255)
                                                                                                 gTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGTTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCcgc                                                <  7:33249/149‑1 (MQ=255)
                                                                                                    aaTTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGt                                              <  1:287916/148‑1 (MQ=255)
                                                                                                        gggTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGa                                                                                        <  7:87506/102‑1 (MQ=255)
                                                                                                        gggTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGa                                                                                        >  8:87506/1‑102 (MQ=255)
                                                                                                           tGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCTTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTg                                      <  6:325208/149‑1 (MQ=255)
                                                                                                              ccGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGTGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGaa                                                                 <  4:93935/119‑1 (MQ=255)
                                                                                                              ccGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGTGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGaa                                                                 >  3:93935/1‑119 (MQ=255)
                                                                                                                 aCATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGa                                                                                        <  6:33976/93‑1 (MQ=255)
                                                                                                                   aTCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTg                               >  8:86294/1‑148 (MQ=255)
                                                                                                                      ttctCGTTACGCCTGATCAACCATAGTGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCAt                            >  6:78894/1‑148 (MQ=255)
                                                                                                                           gTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGATGACCTTGTCGGAAACACCCTTGACGGGCATGCGCCTGTTCTGGAAAACCCTTGAAGACCTGGTGCTGGCCGCGTGGATTTGGCTGTTTGCATCACCCg                      >  3:239230/1‑149 (MQ=255)
                                                                                                                               cGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTAtt                  >  7:229985/1‑149 (MQ=255)
                                                                                                                                gCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTg                 >  8:283412/1‑149 (MQ=255)
                                                                                                                                 ccTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTgc                >  4:131308/1‑149 (MQ=255)
                                                                                                                                     aTCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTgctgg             <  3:131308/148‑1 (MQ=255)
                                                                                                                                     aTCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTgctg              <  7:283412/147‑1 (MQ=255)
                                                                                                                                         aCCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTGCTGGCTGtt        <  7:86294/149‑1 (MQ=255)
                                                                                                                                               gcgtgcgtGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAACCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTGCTGGCTGTTGCGCTg  <  8:68653/149‑1 (MQ=255)
                                                                                                                                                  tgcgtgAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTgct               >  2:154621/1‑133 (MQ=255)
                                                                                                                                                  tgcgtgAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTgct               >  2:149813/1‑133 (MQ=255)
                                                                                                                                                  tgcgtgAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTgct               <  1:154621/133‑1 (MQ=255)
                                                                                                                                                  tgcgtgAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTgct               <  1:149813/133‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGTCATCAACGATGTGTACTTCAAATTGCTCGACAAGTGCATTGCGGTCAATTGGGTGCCCGACATCTTCTCGTTACGCCTGATCAACCATAGCGTGCGTGAAATTGCCGGCATTCCGGTGCTGACCTTGTCGGAAACACCCTTGACGGGCATGAGCCTGTTCTTGAAAAACCTTGAAGACCGGGTGCTGGCCGCGTTGATTTTGCTGTTTGCATCACCCGTATTGCTGGCTGTTGCGCTG  >  NC_002947/3558331‑3558622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑