Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,683,003 A→G 10.0% K72R (AAG→AGG)  PP_3249 → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,683,0030AG10.0% 107.7 / 3.3 40K72R (AAG→AGG) PP_3249putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (19/17);  new base G (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

GGCTGCACGCTGCCGCGCCGGCGGGTTTGCTTGCGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATCGCCGCCAATAGCCGGGCTGAA  >  NC_002947/3682856‑3683151
                                                                                                                                                   |                                                                                                                                                    
ggCTGCACGCTGCCGCGCCGGCGGGTTTGCTTGCGCGCAAAGTCCCGTCCACCGGTGAATCGTTGCCGGAGATCGGTGCCGGTACTTCGGGCAGTTTCGAGTTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCaa                                                                                                                                                      <  6:253191/148‑1 (MQ=255)
ggCTGCACGCTGCCGCGCCGGCGGGTTTGCTTGCGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAg                                                                                                                                                     >  7:4833/1‑149 (MQ=255)
                  cggcggGTTTGCTTGCGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAggcggc                                                                                                                                   <  8:4833/149‑1 (MQ=255)
                         tttGCTTGCGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCggg                                                                                                                            <  3:306600/149‑1 (MQ=255)
                         ttggcttgcGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTGCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCggg                                                                                                                            <  3:301845/146‑1 (MQ=255)
                              ttgcGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAGGGCATTTTTCGAAGGCGGCGGCCGGGt                                                                                                                           <  6:92031/145‑1 (MQ=255)
                              ttgcGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAGGGCATTTTTCGAAGGCGGCGGCCGGGt                                                                                                                           >  5:92031/1‑145 (MQ=255)
                                gcgcgcAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATc                                                                                                                       <  7:74616/147‑1 (MQ=255)
                                                 cACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACg                                                                                                    >  6:274788/1‑149 (MQ=255)
                                                            cGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGa                                                                                          >  4:306885/1‑148 (MQ=255)
                                                                  cGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGCCAGCAtt                                                                                   >  6:156120/1‑149 (MQ=255)
                                                                        tCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGc                                                                             <  3:306885/149‑1 (MQ=255)
                                                                          ggTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCa                                                                           <  8:69714/149‑1 (MQ=255)
                                                                                       cGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAagag                                                              >  8:1258/1‑149 (MQ=255)
                                                                                           cAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGAGAGCATTCTCGGCCAGTTGCTGGAAGAGggcg                                                          >  3:44048/1‑149 (MQ=255)
                                                                                                          aaaCGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGt                                           <  5:274788/149‑1 (MQ=255)
                                                                                                           aaCGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTg                                          >  4:183936/1‑149 (MQ=255)
                                                                                                           aaCGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTg                                          >  4:201249/1‑149 (MQ=255)
                                                                                                            aCGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGc                                         <  3:59026/149‑1 (MQ=255)
                                                                                                             cGGTTCGCCCCAGTACCTTCAGCTCAAAACTGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCt                                        >  6:210560/1‑149 (MQ=255)
                                                                                                               gTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTc                                      <  3:183936/149‑1 (MQ=255)
                                                                                                                   gCCCCAGTACCTTCAGCTCAAAACGGTGCTCTAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTg                                   <  2:9022/148‑1 (MQ=255)
                                                                                                                   gCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTgg                                  >  8:211135/1‑149 (MQ=255)
                                                                                                                   gCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTg                                   >  1:9022/1‑148 (MQ=255)
                                                                                                                             cTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGgc                                                                                               <  1:173715/78‑1 (MQ=255)
                                                                                                                             cTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGgc                                                                                               >  2:173715/1‑78 (MQ=255)
                                                                                                                             cTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTc                                      >  7:124049/1‑135 (MQ=255)
                                                                                                                             cTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTc                                      <  8:124049/135‑1 (MQ=255)
                                                                                                                              ttCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTg                                   <  8:217840/137‑1 (MQ=255)
                                                                                                                              ttCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTg                                   >  7:217840/1‑137 (MQ=255)
                                                                                                                              ttCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATc                       >  2:151210/1‑149 (MQ=255)
                                                                                                                               tCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATcg                      >  3:236709/1‑149 (MQ=255)
                                                                                                                                  gCTCAAAACTGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATcgccg                   <  5:210560/149‑1 (MQ=255)
                                                                                                                                  gCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATcgccg                   >  8:33918/1‑149 (MQ=255)
                                                                                                                                     cAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAACATCgccgcc                 >  3:6351/1‑148 (MQ=255)
                                                                                                                                      aaaaCGGTGCTCAGGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGa                                                                  >  3:122760/1‑98 (MQ=255)
                                                                                                                                      aaaaCGGTGCTCAGGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGa                                                                  <  4:122760/98‑1 (MQ=255)
                                                                                                                                      aaaaCGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTg                                   <  1:142506/129‑1 (MQ=255)
                                                                                                                                      aaaaCGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTg                                   >  2:142506/1‑129 (MQ=255)
                                                                                                                                            gTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATCGCCGCCAATAGCCg         <  4:89816/149‑1 (MQ=255)
                                                                                                                                               cTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATCGCCGCCAATAGCCggg       <  4:236709/148‑1 (MQ=255)
                                                                                                                                                   aGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATCGCCGCCAATAGCCGGGCTGaa  <  6:135459/149‑1 (MQ=255)
                                                                                                                                                   aGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATCGCCGCCAATAGCCGGGCTGaa  <  3:201249/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGCTGCACGCTGCCGCGCCGGCGGGTTTGCTTGCGCGCAAAGTCCCGTCCACCGGTGAAGCGTTGCCGGTGATCGGTGCCGGTACTTCGGGCAGTTTCGAGGTAGAAAACGGTTCGCCCCAGTACCTTCAGCTCAAAACGGTGCTCAAGGCATTTTTCGAAGGCGGCGGCCGGGTGATCGACACCTCGCCCAACTACGGGGGCGCCGACAGCATTCTCGGCCAGTTGCTGGAAGAGGGCGGCTGGCACCGCCAGTGCTTCCTGGCCACCAAGATCGCCGCCAATAGCCGGGCTGAA  >  NC_002947/3682856‑3683151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑