Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,763,083 C→T 13.7% T109I (ACC→ATC)  PP_3326 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,763,0830CT13.7% 129.3 / 10.8 51T109I (ACC→ATC) PP_3326hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base T (3/4);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGAC  >  NC_002947/3762936‑3763230
                                                                                                                                                   |                                                                                                                                                   
ccGGGATCATGTTCCCGGTCACGTTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAcc                                                                                                                                                    >  7:84220/1‑149 (MQ=255)
 cGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAcc                                                                                                                                                    >  6:74067/1‑148 (MQ=255)
       cATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTc                                                                                                                                              >  6:274425/1‑148 (MQ=255)
        aTGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTTGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCaa                                                                                                                                            <  7:265830/149‑1 (MQ=255)
         tGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCATTCAGGATCGCACCCATGTCAAg                                                                                                                                           <  2:112324/149‑1 (MQ=255)
           aTCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGa                                                                                                                                         <  7:54043/149‑1 (MQ=255)
            tCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGAt                                                                                                                                        >  5:84649/1‑149 (MQ=255)
                gggcACGGTCGAGCGCCTGGCGGAGCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGGCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGa                                                                                                                                    <  5:109939/146‑1 (MQ=255)
                  tCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGATc                                                                                                                                  <  4:250323/149‑1 (MQ=255)
                                 tGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGc                                                                                                                   >  3:22237/1‑149 (MQ=255)
                                    cGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTg                                                                                                                <  5:274425/149‑1 (MQ=255)
                                       aTCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGaaa                                                                                                             <  4:24260/149‑1 (MQ=255)
                                            gCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAg                                                                                                        >  1:26892/1‑149 (MQ=255)
                                              cGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGa                                                                                                                                    <  3:1547/119‑1 (MQ=255)
                                              cGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGa                                                                                                                                    >  4:1547/1‑119 (MQ=255)
                                                   tgcAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGc                                                                                                  >  5:273834/1‑148 (MQ=255)
                                                   tgcAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGc                                                                                                  >  5:250708/1‑148 (MQ=255)
                                                          aCTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTAcc                                                                                          <  4:22237/149‑1 (MQ=255)
                                                                 ccGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCgg                                                                                   >  2:94008/1‑149 (MQ=255)
                                                                          gCACTTGCCGTGCTGCTGGACAATGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCt                                                                          >  2:181784/1‑149 (MQ=255)
                                                                            aCTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGcc                                                                                                      <  2:211737/119‑1 (MQ=255)
                                                                            aCTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGcc                                                                                                      >  1:211737/1‑119 (MQ=255)
                                                                                gCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGa                                                                     >  3:98166/1‑148 (MQ=255)
                                                                                gCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGa                                                                     >  1:198982/1‑148 (MQ=255)
                                                                                  cGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAAc                                                                  <  8:84220/149‑1 (MQ=255)
                                                                                  cGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAAc                                                                  <  2:26892/149‑1 (MQ=255)
                                                                                    tgctgcTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTg                                                                >  2:177977/1‑149 (MQ=255)
                                                                                        gctgGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAAc                                                                  <  8:212991/143‑1 (MQ=255)
                                                                                        gctgGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAAc                                                                  >  7:212991/1‑143 (MQ=255)
                                                                                         ctgGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAAAGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGAc                                                             <  5:272017/147‑1 (MQ=255)
                                                                                            gACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACAtgct                                                        >  3:13880/1‑149 (MQ=255)
                                                                                               aaCGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCg                                                     <  1:94008/149‑1 (MQ=255)
                                                                                                  gggCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGttt                                                  >  3:88907/1‑149 (MQ=255)
                                                                                                      cTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGc                                                                                                       >  4:1003/1‑92 (MQ=255)
                                                                                                      cTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGc                                                                                                       <  3:1003/92‑1 (MQ=255)
                                                                                                         ggTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGAt                                            <  1:177977/148‑1 (MQ=255)
                                                                                                           tCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACGTGCTGCGTTTGGCGa                                             <  6:293004/145‑1 (MQ=255)
                                                                                                           tCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACGTGCTGCGTTTGGCGa                                             >  5:293004/1‑145 (MQ=255)
                                                                                                               aGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACAtgctgc                                                      <  7:297999/132‑1 (MQ=255)
                                                                                                               aGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACAtgctgc                                                      >  8:297999/1‑132 (MQ=255)
                                                                                                               aGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTccc                                     <  6:250708/149‑1 (MQ=255)
                                                                                                                        aCAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGAc                            <  6:84649/149‑1 (MQ=255)
                                                                                                                          aaGCAATGGGTCAATCTGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGAccc                          >  6:158405/1‑149 (MQ=255)
                                                                                                                                      aaTCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCgg              >  6:156995/1‑149 (MQ=255)
                                                                                                                                        tCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACAtgct                                                        >  6:7463/1‑105 (MQ=255)
                                                                                                                                        tCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCg                                                     <  6:149259/108‑1 (MQ=255)
                                                                                                                                        tCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCg                                                     >  5:149259/1‑108 (MQ=255)
                                                                                                                                            gATCGCATCCTTGACATGGGTGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGa                                             >  7:230006/1‑112 (MQ=255)
                                                                                                                                            gATCGCATCCTTGACATGGGTGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGa                                             <  8:230006/112‑1 (MQ=255)
                                                                                                                                            gATCGCATCCTTGACATGGGTGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAgg        >  2:97240/1‑149 (MQ=255)
                                                                                                                                            gATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAgg        <  4:13880/149‑1 (MQ=255)
                                                                                                                                              tCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGc       >  2:13304/1‑148 (MQ=255)
                                                                                                                                                  aCCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGAc  <  5:158405/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGAC  >  NC_002947/3762936‑3763230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑