Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,798,255	C→T	6.6%	V215V (GTG→GTA)	PP_3358 ←	hydroxycinnamoyl‑CoA hydratase‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,798,255	0	C	T	6.6%	178.0 / 2.1	61	V215V (GTG→GTA)	PP_3358	hydroxycinnamoyl‑CoA hydratase‑lyase
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base T (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

GAGGAACTGCTTCATGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCATGATGTAGTACAG  >  NC_002947/3798114‑3798398                                                                                                                                               |                                                                                                                                                gAGGAACTGCTTCATGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCaccacc                                                                                                                                           <  3:134080/148‑1 (MQ=255) gAGGAACTGCTTCATGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCg                                                                                                                                          >  2:233636/1‑149 (MQ=255)      aCTGCTTCATGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACgcagc                                                                                                                                                 <  4:127126/137‑1 (MQ=255)      aCTGCTTCATGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACgcagc                                                                                                                                                 >  3:127126/1‑137 (MQ=255)       cTGCTTCATGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCt                                                                                                                                    >  7:235353/1‑149 (MQ=255)             cctGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCCGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCca                                                                                                                              <  7:122689/147‑1 (MQ=255)                gCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCcag                                                                                                                             >  4:6967/1‑147 (MQ=255)                 cccTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACGCCTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCcagca                                                                                                                           <  1:233636/149‑1 (MQ=255)                       tCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCca                                                                                                                              <  8:87613/139‑1 (MQ=255)                       tCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCca                                                                                                                              >  7:87613/1‑139 (MQ=255)                               gccgGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACt                                                                                                            >  6:144205/1‑149 (MQ=255)                                   gTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTcgcg                                                                                                        >  6:140636/1‑149 (MQ=255)                                       tgtCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGggtg                                                                                                    >  2:32615/1‑149 (MQ=255)                                          ccAGCACGCGCGACTGGTCGAGCTTGACGTAGAGGTAGTCCTCGTTCACTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGACATTCTTCTCGGAACGCAGCACCACAGCGTTCTTTTCCAGCAGGTTCAGCGCCAACTCCCGGGTGttt                                                                                                 >  6:285471/1‑149 (MQ=255)                                           cagcagGCGCGACTGGTCGATCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCCCGCAGCACCACCGGGTTCTTTTCCAGCAGGTTAAGCGCCCACTCGAGGGTGGttt                                                                                                >  6:216331/1‑149 (MQ=255)                                               agGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACg                                                                                            <  1:32615/149‑1 (MQ=255)                                                 gcgcGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGTACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCAc                                                                                             <  3:76316/146‑1 (MQ=255)                                                 gcgcGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGTACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCAc                                                                                             <  3:80570/146‑1 (MQ=255)                                                 gcgcGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGTACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCAc                                                                                             >  4:80570/1‑146 (MQ=255)                                                 gcgcGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGTACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCAc                                                                                             >  4:76316/1‑146 (MQ=255)                                                   gcgACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGc                                                                                        >  6:180010/1‑149 (MQ=255)                                                      aCTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCg                                                                                     <  8:235353/149‑1 (MQ=255)                                                              aGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCgg                                                                             <  3:6967/149‑1 (MQ=255)                                                                        agagGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCg                                                                   >  1:243666/1‑149 (MQ=255)                                                                        agagGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCg                                                                   >  3:43414/1‑149 (MQ=255)                                                                          agGTTGTCCTCTTTCTGTTCCGAGGTCAGATGGCGGCAACG‑GTTGATGCCATTGTTCGCGTCACGCTTGACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCATCTCGCTTGTGGTTTCACTCAGCTCGGCCAGCGGCACACTGTCGt                                                                  <  5:285471/148‑1 (MQ=255)                                                                            gTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCa                                                               <  5:144205/149‑1 (MQ=255)                                                                            gTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCAGGTGGTTTCACGCAGCTCg                                                                                     >  1:289021/1‑127 (MQ=255)                                                                            gTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCAGGTGGTTTCACGCAGCTCg                                                                                     <  2:289021/127‑1 (MQ=255)                                                                               ggccTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCa                                                            <  8:150172/147‑1 (MQ=255)                                                                                tCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAg                                                           >  6:285336/1‑149 (MQ=255)                                                                                tCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAg                                                           <  5:84884/149‑1 (MQ=255)                                                                                 ccTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCcag                                                                                                                             >  4:290027/1‑82 (MQ=255)                                                                                 ccTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCcag                                                                                                                             <  3:290027/82‑1 (MQ=255)                                                                                 ccTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTATCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAgg                                                          >  7:223267/1‑149 (MQ=255)                                                                                  cTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGc                                                         >  1:75502/1‑149 (MQ=255)                                                                                  cTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGc                                                         <  5:285336/149‑1 (MQ=255)                                                                                      ttCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACAACAGGGTTCTTTTCAAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACAAGGCCCa                                                      >  3:186933/1‑148 (MQ=255)                                                                                       tCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACACGGCCCAtc                                                    >  6:222314/1‑149 (MQ=255)                                                                                           ttCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCTCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCgg                                                >  6:154691/1‑149 (MQ=255)                                                                                              ccAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTcc                                                                                                                               <  4:87809/67‑1 (MQ=255)                                                                                              ccAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTcc                                                                                                                               >  3:87809/1‑67 (MQ=255)                                                                                              ccAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGGAg                                             >  4:158666/1‑149 (MQ=255)                                                                                               cAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTGCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGc                                            <  4:43999/149‑1 (MQ=255)                                                                                                          gcgGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCAt                                 <  3:158666/149‑1 (MQ=255)                                                                                                               aaCG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAg                            <  5:140636/149‑1 (MQ=255)                                                                                                                 cG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGt                          <  5:154691/149‑1 (MQ=255)                                                                                                                  g‑CTTGAAGCCAATCTTCGCGGCACGCAGCACCACCTGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTc                         <  4:186933/149‑1 (MQ=255)                                                                                                                     ttGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAg                            <  3:302405/144‑1 (MQ=255)                                                                                                                     ttGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAg                            >  4:302405/1‑144 (MQ=255)                                                                                                                             aTTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTGCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGt                 >  8:181819/1‑147 (MQ=255)                                                                                                                                 ttcGCGGCACGCAGCCCCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTGCACCCGGCCCCTCTCGGCCGCCTTGCGACCAGCGCAGGTCTTg                      >  2:304408/1‑138 (MQ=255)                                                                                                                                 ttcGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTc                                                                    >  8:281923/1‑92 (MQ=255)                                                                                                                                 ttcGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTc                                                                    <  7:281923/92‑1 (MQ=255)                                                                                                                                 ttcGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTg                      <  1:309291/138‑1 (MQ=255)                                                                                                                                 ttcGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTg                      >  2:309291/1‑138 (MQ=255)                                                                                                                                 ttcGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTg                      <  1:304408/138‑1 (MQ=255)                                                                                                                                 ttcGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCatgat           >  7:195277/1‑149 (MQ=255)                                                                                                                                   cgcgGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCatgatg          >  2:218116/1‑148 (MQ=255)                                                                                                                                    gcgGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCATGATgta        <  2:75502/149‑1 (MQ=255)                                                                                                                                      ggCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCATGATgtagt      <  5:222314/149‑1 (MQ=255)                                                                                                                                      ggCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCATGATgtag       >  6:139175/1‑148 (MQ=255)                                                                                                                                          cGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCATGATGTAGTACAg  <  8:195277/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                GAGGAACTGCTTCATGCCCTGCTCGCGGCCGCCGGTAGTGTCCAGCAGGCGCGACTGGTCGAGCTTGGCGTAGAGGTAGTCCTCGTTCTGTTCCCAGGTCAGCTCGCGGCAACG‑CTTGAAGCCATTCTTCGCGGCACGCAGCACCACCGGGTTCTTTTCCAGCAGGTTCAGCGCCAACTCGCGGGTGGTTTCACGCAGCTCGGCCAGCGGCACACTGTCGTTCACCAGGCCCATCTCGGCAGCCTTGCGACCATCGAAGGTCTTGCCGGTCATGATGTAGTACAG  >  NC_002947/3798114‑3798398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑