Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,866,365 A→C 9.5% H171Q (CAT→CAG)  PP_3415 ← LacI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,866,3650AC 9.5% 122.0 / 5.1 42H171Q (CAT→CAG) PP_3415LacI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (18/20);  new base C (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GCAGCCTGCAAACCAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTCGAAGCCGGTCAGCAAAATGCCGCAGGGCT  >  NC_002947/3866222‑3866504
                                                                                                                                               |                                                                                                                                           
gCAGCCTGCAAACCAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGAtgctg                                                                                                                                        >  4:135893/1‑149 (MQ=255)
    ccTGCAAACCAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCgag                                                                                                                                    <  2:20497/149‑1 (MQ=255)
        cAAACCAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGaa                                                                                                                                  <  1:200174/147‑1 (MQ=255)
        cAAACCAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAg                                                                                                                                 <  3:214963/148‑1 (MQ=255)
             cAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGc                                                                                                                                <  4:182974/144‑1 (MQ=255)
             cAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGc                                                                                                                                >  3:182974/1‑144 (MQ=255)
                       cAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGccc                                                                                                                 >  3:19279/1‑149 (MQ=255)
                       cAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGccc                                                                                                                 >  3:15136/1‑149 (MQ=255)
                        aaaGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGcccc                                                                                                                <  2:713/149‑1 (MQ=255)
                         aaGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCt                                                                                                               <  6:136158/149‑1 (MQ=255)
                            ccTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGc                                                                                                            >  1:96000/1‑149 (MQ=255)
                                    aaaCCCTCGGCTCGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAg                                                                                                                                 >  5:271791/1‑120 (MQ=255)
                                    aaaCCCTCGGCTCGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAg                                                                                                                                 <  6:271791/120‑1 (MQ=255)
                                      aCCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGa                                                                                                  <  4:19279/149‑1 (MQ=255)
                                      aCCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGa                                                                                                  <  4:15136/149‑1 (MQ=255)
                                        ccTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAACACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTg                                                                                                              >  2:297185/1‑135 (MQ=255)
                                        ccTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAACACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTg                                                                                                              <  1:297185/135‑1 (MQ=255)
                                              cACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCcatc                                                                                          <  3:135893/149‑1 (MQ=255)
                                                           ccTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGcacac                                                                                >  3:105432/1‑146 (MQ=255)
                                                           ccTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGcacac                                                                                <  4:105432/146‑1 (MQ=255)
                                                               gggTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCa                                                                          <  1:213610/148‑1 (MQ=255)
                                                                     aGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCt                                                                   >  7:2488/1‑149 (MQ=255)
                                                                      gCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTg                                                                  <  6:289907/149‑1 (MQ=255)
                                                                             ggCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTg                                                                  <  8:51099/142‑1 (MQ=255)
                                                                             ggCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTg                                                                  >  7:51099/1‑142 (MQ=255)
                                                                                              gcTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGgcagca                                          >  7:298874/1‑149 (MQ=255)
                                                                                                ttGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGc                                                                                                                                <  2:265059/61‑1 (MQ=255)
                                                                                                ttGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGc                                                                                                                                >  1:265059/1‑61 (MQ=255)
                                                                                                      ccGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTgcg                                  >  3:27901/1‑149 (MQ=255)
                                                                                                              gATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTgcg                                  <  2:37133/141‑1 (MQ=255)
                                                                                                              gATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTgcg                                  >  1:37133/1‑141 (MQ=255)
                                                                                                              gATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCAGCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTCGAAGc                          >  3:223849/1‑149 (MQ=255)
                                                                                                                  aGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTCGAAGCCgg                       <  8:2488/148‑1 (MQ=255)
                                                                                                                           gcagcatcaGGCCGGGCGTGCTGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTc                               >  7:64757/1‑131 (MQ=255)
                                                                                                                           gcagcatcaGGCCGGGCGTGCTGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTc                               >  7:69098/1‑131 (MQ=255)
                                                                                                                           gcagcatcaGGCCGGGCGTGCTGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTc                               <  8:64757/131‑1 (MQ=255)
                                                                                                                           gcagcatcaGGCCGGGCGTGCTGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTc                               <  8:69098/131‑1 (MQ=255)
                                                                                                                                     gCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGgca                                             >  4:205624/1‑107 (MQ=255)
                                                                                                                                     gCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGgca                                             <  3:205624/107‑1 (MQ=255)
                                                                                                                                     gCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTgcg                                  >  6:125754/1‑118 (MQ=255)
                                                                                                                                     gCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTgcg                                  <  5:125754/118‑1 (MQ=255)
                                                                                                                                       ccGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTCGAAGCCGGTCAGCAAAATGCCGCAGGGCt  >  4:78751/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                           
GCAGCCTGCAAACCAGCCTCTGCCAAAGCCTGACGGAAACCCTCGGCACGCTGCATCACCCTCGGGTCCAGCTGCGCGGCAATGAATGCAAGGCGCTTGCGCCCGCGCTCGATCAGGTGCCGTGCAGCAGCACGCCCGGCCTGATGCTGCGAGAAGCCGACCGACAAAGCCCCTGGCTCGCCATTGAGCTCCATCATGTGCACACACGGCACGCCGCTGGCAACCAGCATCTGCCGGGCAGCATCGCTGCGCTCGAAGCCGGTCAGCAAAATGCCGCAGGGCT  >  NC_002947/3866222‑3866504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑