Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,941,173 A→G 8.0% G9G (GGT→GGC
*129Q (TAA→CAA) 
PP_3475 ←
PP_3476 ←
type II secretion system pseudopilin PulG‑like signal peptide protein
type II secretion system protein G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,941,1730AG 8.0% 136.4 / 3.3 50G9G (GGT→GGC
*129Q (TAA→CAA) 
PP_3475
PP_3476
type II secretion system pseudopilin PulG‑like signal peptide protein
type II secretion system protein G
Reads supporting (aligned to +/- strand):  ref base A (27/19);  new base G (2/2);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

TACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAG  >  NC_002947/3941030‑3941320
                                                                                                                                                |                                                                                                                                                    
tACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCAtt                                                                                                                                                 <  5:126987/149‑1 (MQ=255)
         ccGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGaa                                                                                                                                          >  4:93892/1‑147 (MQ=255)
                  aGCAACTGGCGTTCGCGGTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCAt                                                                                                                              >  2:260514/1‑149 (MQ=255)
                             ttCGCGGTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGccctc                                                                                                                   >  2:215049/1‑149 (MQ=255)
                             ttCGCGGTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGccctc                                                                                                                   >  5:115357/1‑149 (MQ=255)
                                  ggTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTTAGCGAAGAGGCTGCCATCACGCGCCCTCCCtgt                                                                                                              <  2:114136/149‑1 (MQ=255)
                                  ggTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCtgt                                                                                                              >  4:242573/1‑149 (MQ=255)
                                  ggTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCtgt                                                                                                              <  1:215049/149‑1 (MQ=255)
                                    tCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCAcgcg                                                                                                                        <  5:173430/137‑1 (MQ=255)
                                    tCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCAcgcg                                                                                                                        >  6:173430/1‑137 (MQ=255)
                                              cggcgCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCtt                                                                                                  <  3:93892/149‑1 (MQ=255)
                                               ggcgCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTg                                                                                                 <  2:170697/149‑1 (MQ=255)
                                                gcgcTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGa                                                                                                >  7:134682/1‑149 (MQ=255)
                                                  gcTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACACGCAATCCGCAGCAGCACCCCCAGGTATGTAACGCCGCCGTTGGCGTTACCATTCAGCGAAGAGGCTGCCATCCCGCGCCCTCCCTGTGGCACCGCTCTTGAt                                                                                               >  1:224158/1‑149 (MQ=255)
                                                  gcTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATg                                                                                              >  7:24229/1‑149 (MQ=255)
                                                    tCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTc                                                                                            >  4:29811/1‑149 (MQ=255)
                                                    tCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTc                                                                                            >  3:4731/1‑149 (MQ=255)
                                                      gCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTc                                                                                            <  3:146668/147‑1 (MQ=255)
                                                        aaaGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGc                                                                                         >  6:10496/3‑148 (MQ=255)
                                                                tGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACgccgcc                                                                                >  2:206697/1‑149 (MQ=255)
                                                                   cGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGccgcccgc                                                                             >  6:295785/1‑149 (MQ=255)
                                                                        gccgccAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCacac                                                                        <  2:224158/149‑1 (MQ=255)
                                                                          cgccAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACAccc                                                                      >  6:87970/1‑149 (MQ=255)
                                                                             cAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTGCCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGctc                                                                                                    >  6:10847/1‑116 (MQ=255)
                                                                             cAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTGCCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGctc                                                                                                    >  6:15334/1‑116 (MQ=255)
                                                                             cAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTGCCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGctc                                                                                                    <  5:10847/116‑1 (MQ=255)
                                                                             cAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTGCCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGctc                                                                                                    <  5:15334/116‑1 (MQ=255)
                                                                                   cACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGggcggc                                                             <  5:87970/149‑1 (MQ=255)
                                                                                    ctgctgctGATTGCGGTCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACCCCCACCggccgcg                                                            >  4:1800/3‑149 (MQ=255)
                                                                                             aCCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAg                                                     <  3:29811/147‑1 (MQ=255)
                                                                                             aCCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCt                                                   >  5:43528/1‑149 (MQ=255)
                                                                                                cGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGc                                                 <  8:134682/149‑1 (MQ=255)
                                                                                                   aaTCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAg                                              >  8:283444/1‑149 (MQ=255)
                                                                                                           gcagcaCCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACCCCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGc                                       >  1:82959/1‑149 (MQ=255)
                                                                                                               caCCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTgcgc                                  <  3:242573/149‑1 (MQ=255)
                                                                                                                    ccAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGt                             >  7:274609/1‑149 (MQ=255)
                                                                                                                           tGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCggg                      <  5:295785/149‑1 (MQ=255)
                                                                                                                             tAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACCCCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTc                    >  7:113434/1‑149 (MQ=255)
                                                                                                                             tAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCgg                              >  1:179834/1‑139 (MQ=255)
                                                                                                                             tAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCgg                              <  2:179834/139‑1 (MQ=255)
                                                                                                                              aaaGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCcaca                                                                         >  4:205032/1‑95 (MQ=255)
                                                                                                                              aaaGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCcaca                                                                         <  3:205032/95‑1 (MQ=255)
                                                                                                                                 gccgccGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGACATCGCTGCGCTCGGTGATCGGGTCGAcc                >  5:143058/1‑149 (MQ=255)
                                                                                                                                   cgccgTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCgg              >  8:219023/1‑149 (MQ=255)
                                                                                                                                    gccgTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgt             <  4:4731/149‑1 (MQ=255)
                                                                                                                                    gccgTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgt             <  2:82959/149‑1 (MQ=255)
                                                                                                                                        ttGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGgtgtgt         <  8:274609/149‑1 (MQ=255)
                                                                                                                                          ggCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGtt        <  6:43528/148‑1 (MQ=255)
                                                                                                                                            ccTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTgcg     >  4:91187/1‑149 (MQ=255)
                                                                                                                                            ccTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTgcg     >  1:170472/1‑149 (MQ=255)
                                                                                                                                                aCCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCtgt                                                                                                              >  4:213997/1‑40 (MQ=255)
                                                                                                                                                aCCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCtgt                                                                                                              <  3:213997/40‑1 (MQ=255)
                                                                                                                                                aCCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAg  <  8:24229/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCGGCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCGCTCTTGATGTCGGCCACGCCGCCCGCCACACCCTCGGGCGGCGGCACCAGCTGCCAGGCATCGCTGCGCTCGGTGATCGGGTCGACCGGTGTGTTGCGCAG  >  NC_002947/3941030‑3941320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: