Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 346,804 G→A 6.6% R348C (CGC→TGC)  PP_0287 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947346,8040GA 6.6% 178.2 / 2.1 60R348C (CGC→TGC) PP_0287hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/32);  new base A (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01

CGGTCGGCGTCGAAGGTGTCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGTGCCTTGTCCAGATCACGC  >  NC_002947/346656‑346946
                                                                                                                                                    |                                                                                                                                              
cGGTCGGCGTCGAAGGTGTCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCg                                                                                                                                                <  2:38310/149‑1 (MQ=255)
   tCGGCGTCGAAGGTGTCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGt                                                                                                                                             <  5:246154/149‑1 (MQ=255)
      gCGTCGAAGGTGTCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCAc                                                                                                                                          <  8:86694/149‑1 (MQ=255)
            aaGGTGTCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCAccagctc                                                                                                                                    <  8:200617/149‑1 (MQ=255)
                  tCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCtt                                                                                                                              >  3:261738/1‑149 (MQ=255)
                  tCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCtt                                                                                                                              >  5:319236/1‑149 (MQ=255)
                    gCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTgg                                                                                                                            >  4:30546/1‑149 (MQ=255)
                       cTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCg                                                                                                                          >  6:129589/1‑148 (MQ=255)
                                 aGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCgg                                                                                                               <  4:261738/149‑1 (MQ=255)
                                   gcgcAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGt                                                                                                             <  3:212234/149‑1 (MQ=255)
                                     gcAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCAc                                                                                                                                          <  3:289351/118‑1 (MQ=255)
                                     gcAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCAc                                                                                                                                          >  4:289351/1‑118 (MQ=255)
                                         ggCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGtt                                                                                                         >  4:144486/1‑147 (MQ=255)
                                         ggCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGtt                                                                                                         <  3:144486/147‑1 (MQ=255)
                                         ggCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTtgg                                                                                                       >  3:226596/1‑149 (MQ=255)
                                         ggCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCACGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCCGGTCGTtgg                                                                                                       >  2:101177/1‑149 (MQ=255)
                                                  ggCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGc                                                                                               <  4:143054/148‑1 (MQ=255)
                                                    cGAAATCTTCGACGGCCACGCCGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTAGCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTGACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGAGGCCGGccgg                                                                                            >  8:196356/1‑146 (MQ=255)
                                                     gAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCg                                                                                           >  3:46542/1‑149 (MQ=255)
                                                      aaaTCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCGGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCgg                                                                                          <  8:273160/149‑1 (MQ=255)
                                                        aTCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGt                                                                                        <  4:46542/149‑1 (MQ=255)
                                                           ttCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCatgagg                                                                                         >  1:109138/1‑140 (MQ=255)
                                                           ttCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCgg                                                                                          <  2:109138/144‑1 (MQ=255)
                                                           ttCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGc                                                                                     >  2:154008/1‑149 (MQ=255)
                                                             ctccGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGTCTGCTGGGTGGGCCCAGCAGGCGAGTCAGCAGCTCCACCTTGGCGAAGGCGGCCGGGCGGTTGGTGGCCGGCAGCGGGTGGc                                                                                     <  7:196356/144‑1 (MQ=255)
                                                                cGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATg                                                                                <  1:265628/149‑1 (MQ=255)
                                                                 ggCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGGCGTTGGTGGCCGGCAGCGGGTGGCCAATGc                                                                               <  3:30546/149‑1 (MQ=255)
                                                                  gCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCt                                                                              <  7:297094/149‑1 (MQ=255)
                                                                       gcgGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCTGGTCTTCCAGGTGCAGGCTGCGGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCTGCTTGGGGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCaa                                                                         <  8:201661/149‑1 (MQ=255)
                                                                              cTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGcc                                                                                                  >  6:47089/1‑117 (MQ=255)
                                                                              cTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGcc                                                                                                  <  5:47089/117‑1 (MQ=255)
                                                                               tGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGgcg                                                                 >  3:142724/1‑149 (MQ=255)
                                                                                 aaGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGgcgc                                                                <  4:142724/148‑1 (MQ=255)
                                                                                  aGGTGGTGTCGTCCAGCTTCTCCGCCTGGTCTTCGAGGTCCTGTCTGCTGTGTGTGCCCAGGAGGCGAGTGACCACCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGgcgcg                                                               <  1:101177/148‑1 (MQ=255)
                                                                                   ggTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTtggt                                                                                                      >  4:7729/1‑108 (MQ=255)
                                                                                   ggTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTtggt                                                                                                      <  3:7729/108‑1 (MQ=255)
                                                                                     tGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTTAAGGAAGGCGCGCAgg                                                           <  5:129589/149‑1 (MQ=255)
                                                                                      gCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGt                                                          >  1:174532/1‑149 (MQ=255)
                                                                                          tcgtcCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGaa                                                      >  3:50814/1‑149 (MQ=255)
                                                                                            gtcCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCaagcttg                                                                             <  3:136282/124‑6 (MQ=255)
                                                                                            gtcCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCaagcttg                                                                             >  4:136282/1‑119 (MQ=255)
                                                                                            gtcCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAGTGCTTTCAAGGAAGGCGCGCAGGTTGAAc                                                     <  6:319236/148‑1 (MQ=255)
                                                                                               cAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTggg                                                 >  5:255755/1‑149 (MQ=255)
                                                                                                aGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGc                                                <  1:154008/149‑1 (MQ=255)
                                                                                                aGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGc                                                <  6:255755/149‑1 (MQ=255)
                                                                                                     cACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGAt                                             <  4:226596/147‑1 (MQ=255)
                                                                                                                         ccAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTgg                       <  2:9473/149‑1 (MQ=255)
                                                                                                                             gctgctGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGTg                   >  4:5020/1‑149 (MQ=255)
                                                                                                                                gctgGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGTGCCt                >  4:89123/1‑149 (MQ=255)
                                                                                                                                  tgGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGt                    <  8:159336/143‑1 (MQ=255)
                                                                                                                                  tgGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGt                    >  7:159336/1‑143 (MQ=255)
                                                                                                                                    ggTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGTGCCTTGTc            <  3:89123/149‑1 (MQ=255)
                                                                                                                                             agcagGCAAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTc                                                                           <  4:8268/77‑1 (MQ=255)
                                                                                                                                             agcagGCAAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTc                                                                           <  4:3880/77‑1 (MQ=255)
                                                                                                                                             agcagGCAAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTc                                                                           >  3:8268/1‑77 (MQ=255)
                                                                                                                                             agcagGCAAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTc                                                                           >  3:3880/1‑77 (MQ=255)
                                                                                                                                              gcagGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGTGCCTTGTCCAGATCACGc  <  2:174532/149‑1 (MQ=255)
                                                                                                                                                  gCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTggg                      >  2:132514/1‑125 (MQ=255)
                                                                                                                                                  gCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTggg                      >  2:135357/1‑125 (MQ=255)
                                                                                                                                                  gCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTggg                      <  1:135357/125‑1 (MQ=255)
                                                                                                                                                  gCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTggg                      <  1:132514/125‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CGGTCGGCGTCGAAGGTGTCGCCCTTAAGTTGCAGGCGCAGGGCTTGTTTGGCGAAATCTTCGACGGCCACGCGGCCGCTGAAGGTGCTGTCGTCCAGCTTCACCGCCAGGTCTTCCAGGGCCAGGCTGCTGGGTGTGCCCAGCAGGCGAGTCACCAGCTCCAGCTTGGCGAAGGCGGCCGGGTCGTTGGTGGCCGGCAGCGGGTGGCCAATGCTTTCAAGGAAGGCGCGCAGGTTGAACTGGGCGATGGACAGGCCGCCGCTCAGCTGGGGTGCCTTGTCCAGATCACGC  >  NC_002947/346656‑346946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑