Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,010,958 G→A 16.0% V56I (GTA→ATA)  pobR → transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,010,9580GA16.0% 50.8 / 3.8 25V56I (GTA→ATA) pobRtranscriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (7/14);  new base A (2/2);  total (9/16)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-02

GCGTCCCGTTGTTCAAGCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGACCGTGCATGGCTTTCGCTTCAGCGCCGATATCCAGGGCTATGTCCTGACCTTTGGTACTGCC  >  NC_002947/4010812‑4011104
                                                                                                                                                  |                                                                                                                                                  
gCGTCCCGTTGTTCAAGCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCtgtatgta                                                                                                                                                  <  3:141823/149‑1 (MQ=255)
gCGTCCCGTTGTTCAAGCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCtgtatgt                                                                                                                                                   >  4:141823/1‑148 (MQ=255)
          ggtCAAGCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGc                                                                                                                                         <  3:14099/146‑1 (MQ=255)
             cAAGCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACa                                                                                                                                                >  1:129687/1‑138 (MQ=255)
             cAAGCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACa                                                                                                                                                <  2:129687/138‑1 (MQ=255)
                gCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGccc                                                                                                                                  <  1:316310/149‑1 (MQ=255)
                gCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCAGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGcc                                                                                                                                   >  1:264459/1‑148 (MQ=255)
                      tGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTgg                                                                                                                            <  5:256536/149‑1 (MQ=255)
                      tGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTgg                                                                                                                            <  3:145587/149‑1 (MQ=255)
                        gCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGgag                                                                                                                          <  6:5621/149‑1 (MQ=255)
                                  ggcggggCCCGTCACCGACATTGTGTACTGCGAGTCGATCCCGGCATGAAGCAGCCTGCTTCTCTGGGAGATTAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAgggg                                                                                                                 <  8:208977/143‑1 (MQ=255)
                                         cccGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAg                                                                                                         <  8:128707/149‑1 (MQ=255)
                                          ccGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTACATACAGcagc                                                                                                                                           <  5:77311/114‑4 (MQ=255)
                                          ccGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTACATACAGcagc                                                                                                                                           <  5:81888/114‑4 (MQ=255)
                                          ccGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTACATACAGcagc                                                                                                                                           >  6:77311/1‑111 (MQ=255)
                                          ccGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTACATACAGcagc                                                                                                                                           >  6:81888/1‑111 (MQ=255)
                                                  gACCTGCTGCCCTGCGAGTCGATCCCGGCACGCACCCGCCTGCATCACTGGGAGATCAAGCCGCACCCGCATGCCGAGCTGTTCCCGCTGCTGTATGTACCGCGTGGCCAGGCCCAGGTGGAGATCGAGGCGGAGCGCAGAGCCAtagc                                                                                                >  7:139213/1‑146 (MQ=255)
                                                       gctgcACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGACGAGCTCTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCAACGCCGAAg                                                                                           >  7:106553/1‑149 (MQ=255)
                                                              tGCGAGTCGATCCCGTCACGCTGCCGCCTGCATCAGTGGGAGATCAAGCCGCACCAGCATGCGGAGCTGTTCGAGGTGCTGTTTGTTCAGCGTGGCCAGGGGCTGGTGGAGATCGAGGGGGTGCGCAGTGCGATCGGCGAAgcggcg                                                                                      <  8:139213/147‑1 (MQ=255)
                                                                 gtgtggATCACGGCACGCAGCCGCCTGCATCACTGGGAGATCATGCCGCACCAGTATGCCGTGCTGTTCTAGCTGCTGTATGTACAGGGTGGACAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCa                                                                                 <  8:106553/144‑1 (MQ=255)
                                                                       aTCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGt                                                                           >  1:73473/1‑149 (MQ=255)
                                                                               aCGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGa                                                                   <  2:264459/149‑1 (MQ=255)
                                                                                   aGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGAc                                                               >  3:83184/1‑149 (MQ=255)
                                                                                     ccgccTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGACCg                                                             <  2:73473/149‑1 (MQ=255)
                                                                                      cgccTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGACCGt                                                            <  4:83184/149‑1 (MQ=255)
                                                                                                                      gCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGACCGTGCATGGCTTTCGCTTCAGCGCCGATATCCAgg                            >  3:236753/1‑149 (MQ=255)
                                                                                                                                         ctgctgTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGACCGTGCATGGCTTTCGCTTCAGCGCCGATATCCAGGGCTATGTCCTGACCTTTg          <  4:236753/148‑1 (MQ=255)
                                                                                                                                                 tgtaCAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGACCGTGCATGGCTTTCGCTTCAGCGCCGATATCCAGGGCTATGTCCTGACCTTTGGTACTGcc  >  5:83284/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCGTCCCGTTGTTCAAGCTGTATGGCGAGAATCAGGCCTGGCCCGGCACCGACCTGCTGCACTGCGAGTCGATCCCGGCACGCAGCCGCCTGCATCACTGGGAGATCAAGCCGCACCAGCATGCCGAGCTGTTCCAGCTGCTGTATGTACAGCGTGGCCAGGCCCAGGTGGAGATCGAGGGGGTGCGCAGTGCCATCGGCGAAGCGGCGATCCAGGTCGTGCCACCGATGACCGTGCATGGCTTTCGCTTCAGCGCCGATATCCAGGGCTATGTCCTGACCTTTGGTACTGCC  >  NC_002947/4010812‑4011104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑