Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,022,287 G→A 8.5% R7C (CGC→TGC)  PP_3547 ← NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,022,2870GA 8.5% 126.9 / 2.5 47R7C (CGC→TGC) PP_3547NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base A (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

ATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTG  >  NC_002947/4022140‑4022432
                                                                                                                                                   |                                                                                                                                                 
aTCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCg                                                                                                                                                   >  3:221882/1‑148 (MQ=255)
           gCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCGAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGAc                                                                                                                                       >  3:23811/1‑149 (MQ=255)
               aaTCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACggg                                                                                                                                    >  7:97870/1‑148 (MQ=255)
               aaTCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGc                                                                                                                                   <  5:131026/149‑1 (MQ=255)
                    cgcTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGa                                                                                                                              >  6:270425/1‑149 (MQ=255)
                         cGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGTCATGGATGccc                                                                                                                         <  6:93274/149‑1 (MQ=255)
                         cGTTGGAATGACGAACATCAGCACAGCAAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGc                                                                                                                           <  4:23811/147‑1 (MQ=255)
                          gTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCg                                                                                                                        <  5:15822/149‑1 (MQ=255)
                           ttGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGt                                                                                                                       <  4:308891/149‑1 (MQ=255)
                              gAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTc                                                                                                                      >  7:230245/1‑147 (MQ=255)
                                   aCGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGc                                                                                                                           >  6:7083/1‑137 (MQ=255)
                                   aCGAACAGCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGc                                                                                                                           <  5:7083/137‑1 (MQ=255)
                                     gAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGa                                                                                                             <  1:52667/149‑1 (MQ=255)
                                              cacaGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAgg                                                                                                    <  3:197520/149‑1 (MQ=255)
                                                 aGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAgcgc                                                                                                                >  2:470/1‑134 (MQ=255)
                                                 aGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAgcgc                                                                                                                <  1:470/134‑1 (MQ=255)
                                                  gCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATgg                                                                                                <  7:116942/149‑1 (MQ=255)
                                                   ccAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAg                                                                                                                                            >  6:234541/1‑104 (MQ=255)
                                                   ccAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAg                                                                                                                                            <  5:234541/104‑1 (MQ=255)
                                                   ccAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATggg                                                                                               >  2:133816/1‑149 (MQ=255)
                                                    cAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATgggg                                                                                              <  3:152789/149‑1 (MQ=255)
                                                        ccTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCtt                                                                                          >  4:160722/1‑149 (MQ=255)
                                                               ttttgatctggcttttgatctggcTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGcgcg                                                                                   <  3:160722/149‑1 (MQ=255)
                                                                    atctggcttttgatctggcttttgATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCAGAGGCAGGTg                                                                                                                                         <  6:298200/90‑1 (MQ=255)
                                                                    atctggcttttgatctggcttttgATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCAGAGGCAGGTg                                                                                                                                         <  6:293564/90‑1 (MQ=255)
                                                                    atctggcttttgatctggcttttgATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCAGAGGCAGGTg                                                                                                                                         >  5:293564/1‑90 (MQ=255)
                                                                    atatggcttttgatctggcttttgatcTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCAGAGGCAGGTg                                                                                                                                         >  5:298200/4‑90 (MQ=255)
                                                                      ctggcttttgatctggcttttgatCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGc                                                                            >  4:77152/1‑149 (MQ=255)
                                                                         gcttttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGcgcg                                                                                   <  1:240103/139‑1 (MQ=40)
                                                                           ttttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCgg                                                                         >  2:212024/1‑147 (MQ=38)
                                                                           ttttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCgg                                                                         >  4:250648/1‑147 (MQ=38)
                                                                             ttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGa                                                                       <  3:77152/147‑1 (MQ=37)
                                                                                     gcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACgg                                                                                                                                     <  5:106163/77‑1 (MQ=35)
                                                                                     gcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACgg                                                                                                                                     >  6:106163/1‑77 (MQ=35)
                                                                                     gcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAg                                                                  <  3:5906/144‑1 (MQ=32)
                                                                                               ttgatcttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGaa                                                   >  5:187458/1‑149 (MQ=31)
                                                                                                   tcttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGCCCGGAGCGAGGGAAcccc                                               >  2:239597/1‑149 (MQ=17)
                                                                                                       gatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGa                                            <  3:250648/148‑1 (MQ=32)
                                                                                                          cttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcg                                         >  6:254655/1‑148 (MQ=32)
                                                                                                            tgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcgcag                                      >  1:314256/1‑149 (MQ=32)
                                                                                                                         ttCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGga                         >  7:192090/1‑149 (MQ=32)
                                                                                                                              gaggCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGatgatg                    >  7:274365/1‑149 (MQ=32)
                                                                                                                                 gCCGAACGCTGGCTTTGGGTCGGCTGGTGCGGGGCTTGGTTGCCCGTCATGCGCTGTGGCCCCTGGTTGGGGCCTTGGGCGGGGTCCTGCCGGTAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGa                 <  4:221882/149‑1 (MQ=11)
                                                                                                                                 gCCGAACGCAGGCGTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGAGGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGa                 <  3:194893/149‑1 (MQ=17)
                                                                                                                                    gAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAgcg              >  6:90373/1‑149 (MQ=32)
                                                                                                                                          aGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGt        >  2:32367/1‑149 (MQ=32)
                                                                                                                                                 gCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGtttttt   <  5:90373/147‑1 (MQ=32)
                                                                                                                                                 gCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTg  <  1:239597/147‑1 (MQ=11)
                                                                                                                                                   |                                                                                                                                                 
ATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTG  >  NC_002947/4022140‑4022432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑