Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,043,323 C→T 8.0% E141K (GAG→AAG)  PP_3564 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,043,3230CT 8.0% 144.6 / 2.6 50E141K (GAG→AAG) PP_3564AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (19/27);  new base T (2/2);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

AGCGCACGGGTTCGCCCGCAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGCCTGGTAACG  >  NC_002947/4043175‑4043458
                                                                                                                                                    |                                                                                                                                       
aGCGCACGGGTTCGCCCGCAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTc                                                                                                                                         <  2:133123/149‑1 (MQ=255)
      cGGGTTCGCCCGCAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTc                                                                                                                                   <  5:142777/149‑1 (MQ=255)
               ccGCAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGc                                                                                                                            >  6:63129/1‑147 (MQ=255)
               ccGCAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGGGCCACCGGCGCACGTGATGCGGGTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCa                                                                                                                          <  4:46529/149‑1 (MQ=255)
                 gCAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGt                                                                                                                        <  5:39287/149‑1 (MQ=255)
                  cAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTg                                                                                                                       <  1:301545/149‑1 (MQ=255)
                             cAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACtt                                                                                                            <  5:111400/149‑1 (MQ=255)
                              agtagtGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACttt                                                                                                           >  2:52656/1‑149 (MQ=255)
                                     cAACGGCCAGATCTCTGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGcccc                                                                                                    <  6:120073/149‑1 (MQ=255)
                                      aaCGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCt                                                                                                   <  5:63129/149‑1 (MQ=255)
                                       aCGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTg                                                                                                  >  4:175263/1‑149 (MQ=255)
                                         ggCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACttt                                                                                                           >  5:25895/1‑138 (MQ=255)
                                         ggCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACttt                                                                                                           <  6:25895/138‑1 (MQ=255)
                                           ccAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACttt                                                                                                           <  6:283900/136‑1 (MQ=255)
                                           ccAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTgcag                                                                                               >  6:317948/1‑148 (MQ=255)
                                           ccAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTgcag                                                                                               >  4:52537/1‑148 (MQ=255)
                                                    tGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGcc                                                                                         <  3:137629/145‑1 (MQ=255)
                                                            gTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTgcagca                                                                                             <  4:113371/133‑1 (MQ=255)
                                                            gTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTgcagca                                                                                             >  3:113371/1‑133 (MQ=255)
                                                                                   gTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGAccc                                                      >  5:57889/1‑149 (MQ=255)
                                                                                         aTCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCccac                                                  >  4:280543/1‑147 (MQ=255)
                                                                                         aTCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCccac                                                  >  7:72949/1‑147 (MQ=255)
                                                                                          tCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAg                                               >  7:152333/1‑149 (MQ=255)
                                                                                            gATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCt                                             <  3:228106/149‑1 (MQ=255)
                                                                                                cAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAACCGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGac                                         <  8:125242/149‑1 (MQ=255)
                                                                                                      aaCTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCgca                                                                                      <  2:92347/98‑1 (MQ=255)
                                                                                                      aaCTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCgca                                                                                      >  1:92347/1‑98 (MQ=255)
                                                                                                      aaCTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGc                                   >  7:1854/1‑149 (MQ=255)
                                                                                                           cTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCGCACCAGCTTGACACCCGCCtt                                <  6:57889/147‑1 (MQ=255)
                                                                                                           cTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCtt                                <  3:52537/147‑1 (MQ=255)
                                                                                                              gcgcAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTggcgg                           >  1:164494/1‑149 (MQ=255)
                                                                                                                 cAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGTCCCCCACCAGCTTGACACCCGCCTTGGCGgcct                        <  5:317948/149‑1 (MQ=255)
                                                                                                                 cAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACATCCGCCTTGGCGgcct                        <  8:72949/149‑1 (MQ=255)
                                                                                                                 cAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGgcct                        <  2:164494/149‑1 (MQ=255)
                                                                                                                 cAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGgcct                        <  1:52656/149‑1 (MQ=255)
                                                                                                                   aCAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGgcctgc                      <  6:89431/149‑1 (MQ=255)
                                                                                                                    cAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGgcc                         <  8:73136/145‑1 (MQ=255)
                                                                                                                    cAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACACGCCTTGGCGgcctag                      >  7:73136/1‑146 (MQ=255)
                                                                                                                        tGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAg                 >  5:101962/1‑149 (MQ=255)
                                                                                                                              tGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCCGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGc           >  8:189534/1‑149 (MQ=255)
                                                                                                                                cGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGc           <  3:175263/147‑1 (MQ=255)
                                                                                                                                cGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGCCt         <  8:152333/149‑1 (MQ=255)
                                                                                                                                  gAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGa                  <  4:121176/138‑1 (MQ=255)
                                                                                                                                  gAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGa                  >  3:121176/1‑138 (MQ=255)
                                                                                                                                  gAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGCCTgg       <  8:1854/149‑1 (MQ=255)
                                                                                                                                      cGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGCCTGGTAAc   <  3:280543/149‑1 (MQ=255)
                                                                                                                                       gcagcaCGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGCCTGGTAACg  >  5:286317/1‑149 (MQ=255)
                                                                                                                                             cGAAACTTGGCTTCGTGACGCCAGTGCACAACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGcc          <  2:291971/135‑1 (MQ=255)
                                                                                                                                             cGAAACTTGGCTTCGTGACGCCAGTGCACAACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGcc          <  2:287327/135‑1 (MQ=255)
                                                                                                                                             cGAAACTTGGCTTCGTGACGCCAGTGCACAACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGcc          >  1:291971/1‑135 (MQ=255)
                                                                                                                                             cGAAACTTGGCTTCGTGACGCCAGTGCACAACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGcc          >  1:287327/1‑135 (MQ=255)
                                                                                                                                                    |                                                                                                                                       
AGCGCACGGGTTCGCCCGCAAGCGCGTGACAGTAGTGCAACGGCCAGATCTATGGCGGCGGTGCCACCGGCGCACGTGATGCGGTTGCCATCGATGCAGTACAACTGCTCGCGCAACAGCTGCAACTGCGGAAACGCAGCACGAAACTCGGCTTCGTGACGCCAGTGCACCACCACTTTGTGCCCCTGCAGCAGGCCGCAGGCAGCCAGCAGGAAGGCGCCATTGTCGACCCCCACCAGCTTGACACCCGCCTTGGCGGCCTGCCTGAGCAACGCCTGGTAACG  >  NC_002947/4043175‑4043458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑