Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,129,557 C→T 7.5% C58C (TGC→TGT)  PP_3633 → ArgC‑like N‑acetyl‑gamma‑glutamyl‑phosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,129,5570CT 7.5% 153.5 / 2.2 53C58C (TGC→TGT) PP_3633ArgC‑like N‑acetyl‑gamma‑glutamyl‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base T (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

GGCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGAGCGCATCGCG  >  NC_002947/4129411‑4129704
                                                                                                                                                  |                                                                                                                                                   
ggCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGtgcct                                                                                                                                                   >  5:220223/1‑149 (MQ=255)
           caccacAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGacgacg                                                                                                                                        <  4:280448/149‑1 (MQ=255)
            accacAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGAcgc                                                                                                                                       <  5:170217/149‑1 (MQ=255)
            accacAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGAcgc                                                                                                                                       >  3:236248/1‑149 (MQ=255)
             ccacAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGAcgcc                                                                                                                                      <  1:106585/149‑1 (MQ=255)
                      tGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAg                                                                                                                             >  4:50838/1‑149 (MQ=255)
                        cAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAgg                                                                                                                            <  4:268352/148‑1 (MQ=255)
                        cAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTAGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAgg                                                                                                                            <  4:218531/148‑1 (MQ=255)
                               aTGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGcc                                                                                                                    <  1:220711/149‑1 (MQ=255)
                               aTGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGcc                                                                                                                    >  7:54139/1‑149 (MQ=255)
                                 gCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCg                                                                                                                   >  8:244103/1‑148 (MQ=255)
                                    cGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAAt                                                                                                               <  4:236248/149‑1 (MQ=255)
                                                  gAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAgg                                                                                                 >  1:124380/1‑149 (MQ=255)
                                                       aTCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCgg                                                                                            >  2:307829/1‑149 (MQ=255)
                                                         cTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGt                                                                                          >  3:4073/1‑149 (MQ=255)
                                                          ttCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGt                                                                                          >  7:181406/1‑148 (MQ=255)
                                                            cGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCggg                                                                                           <  3:50838/145‑1 (MQ=255)
                                                             gCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATc                                                                                      <  1:307829/149‑1 (MQ=255)
                                                                  ctgACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGAc                                                                                   <  6:220223/147‑1 (MQ=255)
                                                                     aCCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAg                                                                              <  2:9294/149‑1 (MQ=255)
                                                                         tCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCg                                                                          >  5:247605/1‑149 (MQ=255)
                                                                            ccGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGgcg                                                                       <  3:131722/149‑1 (MQ=255)
                                                                             cGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGgcg                                                                       >  6:87609/1‑148 (MQ=255)
                                                                                   cGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGc                                                                                              <  7:187072/119‑1 (MQ=255)
                                                                                   cGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGc                                                                                              >  8:187072/1‑119 (MQ=255)
                                                                                    gAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGcgcaccgcac                                                               >  5:253888/1‑149 (MQ=255)
                                                                                    gAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGcgcaccgcac                                                               <  7:244103/149‑1 (MQ=255)
                                                                                    gAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCCTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGcgcaccgcac                                                               >  3:164718/1‑149 (MQ=255)
                                                                                                 cGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTgg                                                  <  5:87609/149‑1 (MQ=255)
                                                                                                  gCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTggg                                                 >  6:179577/1‑149 (MQ=255)
                                                                                                       gccgcTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTAt                                            <  1:281405/149‑1 (MQ=255)
                                                                                                        ccgcTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATg                                           <  3:83158/149‑1 (MQ=255)
                                                                                                              cGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCTATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGc                                     >  5:33741/1‑149 (MQ=255)
                                                                                                              cGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGc                                     >  6:235717/1‑149 (MQ=255)
                                                                                                                     aTCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCt                              <  6:247605/149‑1 (MQ=255)
                                                                                                                        aaCAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGgt                                                >  4:42225/1‑128 (MQ=255)
                                                                                                                        aaCAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGgt                                                <  3:42225/128‑1 (MQ=255)
                                                                                                                        aaCAGTGACGACATCGCCCTGCTGTGCCTGCCCGAAGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCCGGTGCGGCTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGa                           >  6:279395/1‑149 (MQ=255)
                                                                                                                                cGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAg                    >  3:306234/1‑148 (MQ=255)
                                                                                                                                 gACATCGCCCTGCTGTGTCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTggg                                                 <  8:270024/118‑1 (MQ=255)
                                                                                                                                 gACATCGCCCTGCTGTGTCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTggg                                                 <  8:265800/118‑1 (MQ=255)
                                                                                                                                 gACATCGCCCTGCTGTGTCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTggg                                                 >  7:270024/1‑118 (MQ=255)
                                                                                                                                 gACATCGCCCTGCTGTGTCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTggg                                                 >  7:265800/1‑118 (MQ=255)
                                                                                                                                 gACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGc                   >  3:252797/1‑148 (MQ=255)
                                                                                                                                   cATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAg                >  5:296742/1‑149 (MQ=255)
                                                                                                                                     tCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGAc                          <  2:136766/137‑1 (MQ=255)
                                                                                                                                     tCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGAc                          >  1:136766/1‑137 (MQ=255)
                                                                                                                                       gCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGa            <  4:252797/149‑1 (MQ=255)
                                                                                                                                       gCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGa            <  8:181406/149‑1 (MQ=255)
                                                                                                                                        cccTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCTATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGAg           <  6:33741/149‑1 (MQ=255)
                                                                                                                                         ccTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTgg                                                  >  6:126541/1‑109 (MQ=255)
                                                                                                                                         ccTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTgg                                                  <  5:126541/109‑1 (MQ=255)
                                                                                                                                         ccTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGAgc          <  2:105153/149‑1 (MQ=255)
                                                                                                                                             ctgTGCCTGCCCGACGACGGCGCCCGCGAGGCCGTGGCCGCAATCCACAAGCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCTAGCCGAGCGCAt      <  4:4073/149‑1 (MQ=255)
                                                                                                                                                tgcctgccCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGAGCGCATcg    <  4:306234/148‑1 (MQ=255)
                                                                                                                                                  cctgccCGACGACGCCGCCCTCGAGGCAGTGGCCGCAATCCACAACCCCAAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACATCAAGCCGAGAGAATcgcc  >  2:307448/1‑147 (MQ=255)
                                                                                                                                                  cctgccCGACGACGCCGCCCGAGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTAGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGAGCGCATcgcg  <  1:307448/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGCGACCAAGGCACCACAGGCCTGCAGATCCATGCCCGCCTGCAGGGCCGGAGCGATCTTCGCCTGCTGACCCTCCCCGAGGCCGAGCGCAAGGACCCGCAACGCCGCTGCGAAGCGATCAACAGTGCCGACATCGCCCTGCTGTGCCTGCCCGACGACGCCGCCCGCGAGGCCGTGGCCGCAATCCACAACCCCCAGGTGCGGGTGATCGACGCCAGCTCGGCGCACCGCACCACACCAGGCTGGGTGTATGGCCTGCCGGAGCTTGACGAACAGCAAGCCGAGCGCATCGCG  >  NC_002947/4129411‑4129704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑