Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 353,067 A→G 100% intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947353,0670AG100.0% 131.8 / NA 41intergenic (+34/+84)hisF/cbcVimidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/21);  total (20/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATC  >  NC_002947/352921‑353205
                                                                                                                                                  |                                                                                                                                          
ttCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCggg                                                                                                                                          >  4:106747/1‑149 (MQ=255)
                cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCACGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTCCca                                                                                                                            >  5:224840/1‑147 (MQ=255)
                cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCca                                                                                                                            >  8:41737/1‑147 (MQ=255)
                 aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                                         <  8:206667/149‑1 (MQ=255)
                 aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                                         <  7:294960/149‑1 (MQ=255)
                        tGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCt                                                                                                                  <  2:129629/149‑1 (MQ=255)
                             gcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTgaga                                                                                                              >  8:50517/1‑148 (MQ=255)
                                gcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCAACCCCCCGCCCACAGGTTTGGGGAGAtaac                                                                                                          >  5:24972/1‑146 (MQ=255)
                                gcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGACCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGAtctc                                                                                                          >  1:64594/1‑149 (MQ=255)
                                  cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTAATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGGTGAGAtgtgtg                                                                                                        >  8:47042/1‑144 (MQ=255)
                                         ttCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGGTTGCTGAGATCTCTGTGGGAGc                                                                                                 >  2:79186/1‑149 (MQ=255)
                                           ccACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGc                                                                                                 <  7:50517/147‑1 (MQ=255)
                                                       gTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGGTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGa                                                                                   <  5:75485/149‑1 (MQ=255)
                                                        tACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcgcg                                                                                    >  1:54756/1‑147 (MQ=255)
                                                          cacaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAg                                                                                 <  4:241368/148‑1 (MQ=255)
                                                           acaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGGTTTGGTGAGCTCTCTGTGGGAGCGGGGGTGCCCGCGAAggg                                                                               >  3:82819/1‑149 (MQ=39)
                                                             aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGcc                                                                             <  2:54756/149‑1 (MQ=255)
                                                               tGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCcgc                                                                           >  7:200533/1‑149 (MQ=255)
                                                                  cGGAGGCCAAGGCCTACATGTCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCTCGGGCACGCCCGCTCCCACAGGTTTGc                                                                                                                   <  7:14596/106‑1 (MQ=255)
                                                                  cGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGATTTGCAGCACTTCGCGGGCACGCCCGCTCCCACAGGTTTGc                                                                                                                   >  8:14596/1‑106 (MQ=255)
                                                                  cGGAGGCCAAGGCCTACATGGCTGCGCGCGGGAGCGTCGTTCGCTGACCCATTGACCGGGGCTGCTTGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGaa                                                                        <  1:208830/149‑1 (MQ=38)
                                                                  cGGAGGCCAAGGCCTACATGGCTCCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTGCGCGGGCACGCCCGCTCCCACCGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGaa                                                                        <  1:172832/149‑1 (MQ=255)
                                                                       ccaatggCCTACTGGGCTTCGCGGGGTATGGTGTTTCGGTGATCATTTGACGGGGGCTGTTTTGCTGCCGTTGGGGGGCAGGCCGGCTCCAACTGGTTTGCTTATATCTTTGTGGTTGGGGGGGTGCCCGCGAAGGGCCGCGAAGCGGc                                                                   <  6:24972/144‑1 (MQ=255)
                                                                         cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                                >  8:106108/1‑102 (MQ=255)
                                                                         cAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTga                                                                                                                <  7:106108/102‑1 (MQ=255)
                                                                                  cttGGCTGCGCGCGGGATCGGCGTTCGCTGACCCTTTGACCGGGGCTGCTTTGCAGCCCTGCGCGGGCACGCCCGCTCCCACAGGTTTGCTTAGAGCTCTGTGGTAGGGGGGGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGc                                                          <  4:82819/145‑1 (MQ=37)
                                                                                   aTGGCTTCGCGCGGGTTCGTCGTTCGCTAATCCATTTACCGGGGCTGCTTTTCAGCCCTTTGCGGGCACGCCCTCTCCCACAGTTTTTCTTTTATTTTTTTGGGTGTGGGTGTGCCCGCGAATGGCCGCGAAGCGGCCCCAATTGCTTc                                                       <  7:47042/149‑1 (MQ=255)
                                                                                             gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTg                                                >  1:68649/1‑146 (MQ=255)
                                                                                                      tcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGag                                    >  5:57752/1‑149 (MQ=255)
                                                                                                            gCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAgcgc                              <  6:57752/149‑1 (MQ=255)
                                                                                                               gagccATTGCCCGGCGCTGCTTTCCAGCCCTTCGCGGGCACGCCCGCTCCCACCGGTTGGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTg                           <  5:256173/146‑1 (MQ=38)
                                                                                                                    aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTaa                       >  4:106997/1‑148 (MQ=255)
                                                                                                                    aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCACCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTa                        >  6:302139/1‑147 (MQ=255)
                                                                                                                    aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCTCCCACAGGTTTGCTGAGATATGTGTGGGAGCGGGCGTGCCCGCGAACGGCCGCCAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTa                        >  4:73605/1‑147 (MQ=38)
                                                                                                                         ccGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg                 <  5:302139/149‑1 (MQ=255)
                                                                                                                         ccGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCGCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg                 <  7:41737/149‑1 (MQ=255)
                                                                                                                                tgctTTGCAGCCCTTCGCGGGCACCCCCCCTCCCCCAGGGTTGGGGAGATATGGGTGGGCGCGGGCGGGCCCGCCCAGGGCCCCCGAGCGGCCCCAATTGCTTAAACCCGGGGTGTTGGCGAGCAGCGCCTGGGAAAGCTACGTATacc          >  2:117803/1‑149 (MQ=255)
                                                                                                                                  ctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGTTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGtt                                             <  5:3187/112‑1 (MQ=255)
                                                                                                                                   tttGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATcccc         >  6:84364/1‑147 (MQ=255)
                                                                                                                                   tttGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATcccc         >  2:83427/1‑147 (MQ=255)
                                                                                                                                     tGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGa       <  5:84364/147‑1 (MQ=255)
                                                                                                                                        aGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATc  <  3:106997/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
TTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATC  >  NC_002947/352921‑353205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑