Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,261,968 T→A 12.3% E265V (GAG→GTG) ‡ PP_3734 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,261,9680TA12.3% 170.9 / 11.1 63E265V (GAG→GTG) ‡PP_3734ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (23/32);  new base A (3/5);  total (26/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCACGCGGCCAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGTTCAAGATA  >  NC_002947/4261821‑4262110
                                                                                                                                                   |                                                                                                                                              
agcCACGCGGCCAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTc                                                                                                                                               <  6:297072/149‑1 (MQ=255)
      gcgGCCAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAgcgc                                                                                                                                         <  7:182133/149‑1 (MQ=255)
          ccAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGGAGCAGCGccgtg                                                                                                                                     <  8:102811/149‑4 (MQ=255)
          ccAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGa                                                                                                                                     >  5:55543/1‑149 (MQ=255)
          ccAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGa                                                                                                                                     >  6:26791/1‑149 (MQ=255)
           cAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGGAGCAGCGccgtgg                                                                                                                                    <  5:181948/149‑5 (MQ=255)
           cAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGGAGCAGCGccgtg                                                                                                                                     >  1:81522/1‑145 (MQ=255)
           cAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGGAGCAGCGccgtg                                                                                                                                     <  2:81522/148‑4 (MQ=255)
              tGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGa                                                                                                                                 >  4:31429/1‑149 (MQ=255)
              tGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGa                                                                                                                                 <  5:285838/149‑1 (MQ=255)
                       aCTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGt                                                                                                                        >  2:231947/1‑149 (MQ=255)
                        cTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTg                                                                                                                       <  6:40649/149‑1 (MQ=255)
                        cTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTg                                                                                                                       <  2:61663/149‑1 (MQ=255)
                           gCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTgg                                                                                                                      <  7:213213/147‑1 (MQ=255)
                              aCCCCGGCCAGCCCACCCAGTGCCGCGAACATGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGGCGCACGCGCTCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCTGCGTGGGCAAt                                                                                                                 <  3:71664/149‑1 (MQ=255)
                              aCCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAAt                                                                                                                 <  6:232828/149‑1 (MQ=255)
                                 ccGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGaa                                                                                                              <  6:97670/149‑1 (MQ=255)
                                    gCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAAt                                                                                                                 <  5:140506/143‑1 (MQ=255)
                                    gCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAAt                                                                                                                 >  6:140506/1‑143 (MQ=255)
                                    gCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACaa                                                                                                           <  7:112406/149‑1 (MQ=255)
                                    gCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACaa                                                                                                           <  5:26791/149‑1 (MQ=255)
                                         cccacccaGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCcga                                                                                                      >  5:21542/1‑149 (MQ=255)
                                             cccaGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCcgacgac                                                                                                  <  6:213027/149‑1 (MQ=255)
                                                      gcgAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCcag                                                                                         >  4:296573/1‑149 (MQ=255)
                                                      gcgAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCcag                                                                                         <  2:259006/149‑1 (MQ=255)
                                                              gCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGcc                                                                                   >  7:140487/1‑147 (MQ=255)
                                                                ccAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAgc                                                                               <  6:55543/149‑1 (MQ=255)
                                                                ccAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAgc                                                                               >  4:257479/1‑149 (MQ=255)
                                                                    cTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCgg                                                                           >  7:235340/1‑149 (MQ=255)
                                                                       cAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGt                                                                          >  3:127862/1‑147 (MQ=255)
                                                                       cAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCa                                                                        <  3:31429/149‑1 (MQ=255)
                                                                         gCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGa                                                                                                               >  7:121641/1‑108 (MQ=255)
                                                                         gCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGa                                                                                                               <  8:121641/108‑1 (MQ=255)
                                                                         gCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAgg                                                                      <  4:139601/149‑1 (MQ=255)
                                                                               gggCGCACAGCTGGGTGTTCAGGCTGACGCCGCAGGCGCGCAGGTTACGGATCAGCCCACGGCGGTGCTCCAGCGCCAGACCGATTGCGGGGTGTGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAgg                                                                <  6:21542/149‑1 (MQ=255)
                                                                                ggCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGt                                                               <  3:257479/149‑1 (MQ=255)
                                                                                     acaGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGaaa                                                          <  8:49165/149‑1 (MQ=255)
                                                                                     acaGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGaaa                                                          <  8:140487/149‑1 (MQ=255)
                                                                                                     gCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCt                                          <  4:127862/149‑1 (MQ=255)
                                                                                                      cTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGACCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTg                                         >  6:103204/1‑149 (MQ=255)
                                                                                                       tGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCcag                                                                                         >  8:202023/1‑100 (MQ=255)
                                                                                                       tGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCcag                                                                                         <  7:202023/100‑1 (MQ=255)
                                                                                                       tGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTg                                         >  1:72356/1‑148 (MQ=255)
                                                                                                         aCGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCaa                                      <  1:231947/149‑1 (MQ=255)
                                                                                                                      gcAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTcc                         >  5:326117/1‑149 (MQ=255)
                                                                                                                        aGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAAc                                                                                              <  4:304542/78‑1 (MQ=255)
                                                                                                                        aGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAAc                                                                                              >  3:304542/1‑78 (MQ=255)
                                                                                                                               ggATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGc                >  6:173186/1‑149 (MQ=255)
                                                                                                                                 aTCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCaa                                      <  3:265197/125‑1 (MQ=255)
                                                                                                                                 aTCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCaa                                      >  4:265197/1‑125 (MQ=255)
                                                                                                                                     gCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGt          >  3:501/1‑149 (MQ=255)
                                                                                                                                     gCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGt          >  3:205011/1‑149 (MQ=255)
                                                                                                                                      ggtctggCGCTGGAGCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCaa                                      <  2:35094/115‑1 (MQ=255)
                                                                                                                                      ggtctggCGCTGGAGCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCaa                                      >  1:35094/6‑120 (MQ=255)
                                                                                                                                      cccACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGcgcg                                                                            <  6:245176/82‑1 (MQ=255)
                                                                                                                                      cccACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGcgcg                                                                            >  5:245176/1‑82 (MQ=255)
                                                                                                                                      cccACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTc                                                 >  3:278906/1‑109 (MQ=255)
                                                                                                                                      cccACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTc                                                 <  4:278906/109‑1 (MQ=255)
                                                                                                                                      cccACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGtt         <  7:26488/149‑1 (MQ=255)
                                                                                                                                        tctggCGCTGGAGCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGTTc        >  1:236868/4‑148 (MQ=255)
                                                                                                                                        tctggCGCTGGAGCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGTTCa       <  2:236868/146‑1 (MQ=255)
                                                                                                                                         aCGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGTTCaa      <  2:72356/149‑1 (MQ=255)
                                                                                                                                             cgcTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGTTCAAGATa  <  8:235340/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AGCCACGCGGCCAATGCGTAACCACTGGCCACCCCGGCCAGCCCACCCAGTGCCGCGAACAAGCCCAGCTCCAGCACCAGGGCGCACAGCAGGGTCTTCAGGCTGACGCCGCACGCGCGCAGGTTACGGATCAGCCCACGGCGCTGCTCCAGCGCCAGACCGATTGCGGCGTGGGCAATGAACAAGCCGACGACGAACGCCAGCAGGCCGAGCGCGGTCAGGTCGAGGTGGAAACTGTCGGTCAGGCGCTGCAAGCCACCATCGTCCTGCCGCGGCTGCAGTTCAAGATA  >  NC_002947/4261821‑4262110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑