Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,270,955	C→T	6.5%	Y365Y (TAC→TAT)	mrdA‑I →	transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,270,955	0	C	T	6.5%	182.6 / 3.1	61	Y365Y (TAC→TAT)	mrdA‑I	transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (29/28);  new base T (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

AACCGTGTCCTGCGCGGCCTGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTGGGCCAGAAG  >  NC_002947/4270809‑4271102                                                                                                                                                    |                                                                                                                                                    aaCCGTGTCCTGCGCGGCCTGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCg                                                                                                                                                   >  4:263428/1‑149 (MQ=255)        tCCTGCGCGGCCTGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTg                                                                                                                                             >  5:293151/1‑148 (MQ=255)              gcgGCCTGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGCATGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCg                                                                                                                                       >  1:233870/1‑149 (MQ=255)               cgGCCTGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCg                                                                                                                                       >  7:86350/1‑147 (MQ=255)                  ccTGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAgcg                                                                                                                                  >  6:275297/1‑149 (MQ=255)                    tGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAgcggc                                                                                                                                >  5:188563/1‑149 (MQ=255)                         gcgcCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACgg                                                                                                                           >  4:131231/1‑149 (MQ=255)                          cgcCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGc                                                                                                                          <  2:233870/149‑1 (MQ=255)                          cgcCCGGCTCGACCGTGAAGCCTGAAGTGACCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACgg                                                                                                                           <  8:298828/148‑1 (MQ=255)                           gcCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCt                                                                                                                         >  6:118500/1‑149 (MQ=255)                                gCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTg                                                                                                                    <  3:263428/149‑1 (MQ=255)                                  tCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGa                                                                                                                  >  4:172314/1‑149 (MQ=255)                                    gACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGa                                                                                                                  <  2:197258/147‑1 (MQ=255)                                      ccGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATg                                                                                                              <  3:172314/149‑1 (MQ=255)                                               cTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTATCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGc                                                                                                      >  5:16266/1‑148 (MQ=255)                                               cTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTATCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGc                                                                                                      >  5:11802/1‑148 (MQ=255)                                                  aaGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCcatc                                                                                                  <  7:91592/149‑1 (MQ=255)                                                    gTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCcatcat                                                                                                <  4:193176/149‑1 (MQ=255)                                                        ccATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGt                                                                                            <  3:131231/149‑1 (MQ=255)                                                         cATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGtt                                                                                           >  5:129435/1‑149 (MQ=255)                                                                  gCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGTCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGa                                                                                    <  5:180370/148‑1 (MQ=255)                                                                  gCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCcatc                                                                                                  <  8:209602/134‑1 (MQ=255)                                                                  gCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCcatc                                                                                                  >  7:209602/1‑134 (MQ=255)                                                                       gACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACAcc                                                                                <  8:86350/147‑1 (MQ=255)                                                                          aGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACt                                                                            >  3:212396/1‑148 (MQ=255)                                                                                        cccTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTa                                                                                                            >  6:64126/1‑102 (MQ=255)                                                                                        cccTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTa                                                                                                            <  5:64126/102‑1 (MQ=255)                                                                                             gCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTgg                                                                >  4:53598/1‑141 (MQ=255)                                                                                             gCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTgg                                                                <  3:53598/141‑1 (MQ=255)                                                                                                gCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTg                                                     <  6:188563/149‑1 (MQ=255)                                                                                                 ccGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTgg                                                    <  6:129435/149‑1 (MQ=255)                                                                                                     gtgtTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCAt                                                <  6:293151/149‑1 (MQ=255)                                                                                                        ttCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTg                                                                 >  5:155063/1‑129 (MQ=255)                                                                                                        ttCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACAGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTg                                                                 <  6:155063/129‑1 (MQ=255)                                                                                                          cGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGTACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTTGGCATCGAc                                            <  4:212396/148‑1 (MQ=255)                                                                                                              ccGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCt                                       >  4:6775/1‑149 (MQ=255)                                                                                                                      ctacGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGAc                                >  4:58850/1‑148 (MQ=255)                                                                                                                      ctacGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGAc                                >  8:66512/1‑148 (MQ=255)                                                                                                                       tacGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTa                              >  5:249475/1‑149 (MQ=255)                                                                                                                       tacGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTa                              >  2:190444/1‑149 (MQ=255)                                                                                                                         cGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTgg                                                                >  7:291442/1‑113 (MQ=255)                                                                                                                         cGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTgg                                                                <  8:291442/113‑1 (MQ=255)                                                                                                                            aCTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATgg                         >  7:255070/1‑149 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAAc                                                                                      <  8:120668/84‑1 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGcc                       <  7:96796/147‑1 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGcc                       >  4:112729/1‑147 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGcc                       >  6:13838/1‑147 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGa                     <  5:118500/149‑1 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGa                     <  3:6775/149‑1 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGa                     <  3:58850/149‑1 (MQ=255)                                                                                                                                cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGa                     <  7:66512/149‑1 (MQ=255)                                                                                                                                    aCTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTc                 >  5:199765/1‑149 (MQ=255)                                                                                                                                          aCCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTg           <  6:249475/149‑1 (MQ=255)                                                                                                                                           ccACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTgg          <  5:13838/149‑1 (MQ=255)                                                                                                                                           ccACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAACTCGGccatg          <  8:93502/149‑4 (MQ=255)                                                                                                                                            cacaAGTATCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTggg         <  6:16266/149‑1 (MQ=255)                                                                                                                                            cacaAGTATCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTggg         <  6:11802/149‑1 (MQ=255)                                                                                                                                             acaAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTGGGc        >  2:75869/1‑149 (MQ=255)                                                                                                                                               aaGTACAGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGAGTTCCAACGACACCTACTTATACGACCTGGCGCACAAGATGGGCATCTACCGGCTGCACGACTACATGGCCGAGTTCGGCCTGGGCCa      >  1:159523/1‑149 (MQ=255)                                                                                                                                                 gTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTGGGCCAg     >  4:277913/1‑148 (MQ=255)                                                                                                                                                 gTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTGGGCCAg     >  1:268325/1‑148 (MQ=255)                                                                                                                                                    ccGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTGGGCCAGAAg  <  1:75869/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AACCGTGTCCTGCGCGGCCTGTATGCGCCCGGCTCGACCGTGAAGCCTGAAGTGGCCATCGC‑TGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGGCCGAGTTCGGCCTGGGCCAGAAG  >  NC_002947/4270809‑4271102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑