Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,351,931 T→G 7.5% K92Q (AAG→CAG)  PP_3824 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,351,9310TG 7.5% 166.0 / 4.1 53K92Q (AAG→CAG) PP_3824hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACATATAAGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAG  >  NC_002947/4351786‑4352070
                                                                                                                                                 |                                                                                                                                           
aaCATATAAGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTc                                                                                                                                          <  1:163822/149‑1 (MQ=255)
aaCATATAAGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTc                                                                                                                                          >  3:245378/1‑149 (MQ=255)
aaCATATAAGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTc                                                                                                                                          >  2:60009/1‑149 (MQ=255)
        aGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATcc                                                                                                                                   <  6:23369/148‑1 (MQ=255)
        aGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATcc                                                                                                                                   <  5:11924/148‑1 (MQ=255)
         gCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCg                                                                                                                                         <  1:107701/141‑1 (MQ=255)
         gCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCg                                                                                                                                         >  2:107701/1‑141 (MQ=255)
                   gaggaCCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCAGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCGTGTCGATATCCAACAGCGCCGCt                                                                                                                       >  4:8708/1‑149 (MQ=255)
                      gaaccGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGAAGCTGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACAAGCGCCGCTgtg                                                                                                                    >  3:42690/4‑147 (MQ=255)
                             tGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCAc                                                                                                             >  6:118791/1‑149 (MQ=255)
                                     aTCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGt                                                                                                      >  3:301398/1‑148 (MQ=255)
                                     aTCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGACAAGGCGCAGCGGGAAGTTATTCAGCCGCTCTTGAAGATTGATGCTGCTGACGCTGCCGAGCTTGTCGATATCCACAAGCGCCTCTGGGCCGGCACCTGAGGt                                                                                                      >  1:208668/1‑148 (MQ=255)
                                       cAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGAt                                                                                                   >  4:22866/1‑149 (MQ=255)
                                       cAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGAt                                                                                                   >  4:18638/1‑149 (MQ=255)
                                        aaCTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCt                                                                                                                       <  2:184963/128‑1 (MQ=255)
                                        aaCTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCt                                                                                                                       >  1:184963/1‑128 (MQ=255)
                                        aaCTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGAt                                                                                                   <  2:151686/148‑1 (MQ=255)
                                             ccGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCt                                                                                              <  4:245378/148‑1 (MQ=255)
                                              cGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTg                                                                                             <  4:301398/148‑1 (MQ=255)
                                                   aTGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTAc                                                                                       >  5:90211/1‑149 (MQ=255)
                                                     gCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGa                                                                                     >  5:89462/1‑149 (MQ=255)
                                                                ccGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAgg                                                                          <  3:18638/149‑1 (MQ=255)
                                                                ccGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAgg                                                                          <  3:22866/149‑1 (MQ=255)
                                                                         gcACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAg                                                                                 >  5:15131/1‑133 (MQ=255)
                                                                         gcACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAg                                                                                 <  6:15131/133‑1 (MQ=255)
                                                                           acaTCGCCAAGGCGCAGCGGGAAGTTATACAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACg                                                               >  1:179059/1‑149 (MQ=255)
                                                                             aTCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCg                                                                                           <  1:188627/119‑1 (MQ=255)
                                                                             aTCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCg                                                                                           >  2:188627/1‑119 (MQ=255)
                                                                              tCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACgctg                                                            <  6:133843/149‑1 (MQ=255)
                                                                                           gaggtaaGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCGGCTGACGCGCTTGTCGATATCCTCCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGATGCTCTTTGGTCAGc                                               <  2:208668/144‑1 (MQ=255)
                                                                                                     aTTCCGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCAcgc                                      <  8:58486/148‑1 (MQ=255)
                                                                                                         aGCCGCTCGTGTAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTc                                                                     >  3:228778/1‑113 (MQ=255)
                                                                                                         aGCCGCTCGTGTAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTc                                                                     <  4:228778/113‑1 (MQ=255)
                                                                                                         aGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCTGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATc                                 <  1:60009/149‑1 (MQ=255)
                                                                                                          gCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCg                                >  7:50153/1‑149 (MQ=255)
                                                                                                          gCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCg                                <  6:90211/149‑1 (MQ=255)
                                                                                                                 gTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCt                         >  7:182098/1‑149 (MQ=255)
                                                                                                                 gTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCt                         >  1:47166/1‑149 (MQ=255)
                                                                                                                    cAGTTTGAAGCTGCTGCCGCTGCCGCGCGTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCtt                        <  3:180433/147‑1 (MQ=255)
                                                                                                                     aGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTtctc                     >  5:326747/1‑149 (MQ=255)
                                                                                                                      gTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTcc                    >  2:118525/1‑149 (MQ=255)
                                                                                                                          gATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCttt                 <  5:118791/148‑1 (MQ=255)
                                                                                                                           aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGctc                                                         >  1:222254/1‑107 (MQ=255)
                                                                                                                           aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGctc                                                         <  2:222254/107‑1 (MQ=255)
                                                                                                                           aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTAc               <  1:118525/149‑1 (MQ=255)
                                                                                                                           aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTAc               >  5:156613/1‑149 (MQ=255)
                                                                                                                           aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACACTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTAc               <  6:89462/149‑1 (MQ=255)
                                                                                                                                       ctgccgcGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATa   <  6:156613/149‑1 (MQ=255)
                                                                                                                                        tgccgcGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAg  >  6:152536/1‑149 (MQ=255)
                                                                                                                                        agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg        <  1:6398/139‑1 (MQ=255)
                                                                                                                                        agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg        >  2:11491/5‑143 (MQ=255)
                                                                                                                                        agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg        >  2:6398/5‑143 (MQ=255)
                                                                                                                                        agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg        <  1:11491/139‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
AACATATAAGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAG  >  NC_002947/4351786‑4352070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑