Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,351,941 C→G 7.7% V88V (GTG→GTC)  PP_3824 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,351,9410CG 7.7% 155.5 / 3.2 52V88V (GTG→GTC) PP_3824hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/22);  new base G (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

AGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGCTCTTGCCA  >  NC_002947/4351794‑4352088
                                                                                                                                                   |                                                                                                                                                   
aGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATcc                                                                                                                                                     <  6:23369/148‑1 (MQ=255)
aGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATcc                                                                                                                                                     <  5:11924/148‑1 (MQ=255)
           gaggaCCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCAGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCGTGTCGATATCCAACAGCGCCGCt                                                                                                                                         >  4:8708/1‑149 (MQ=255)
              gaaccGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGAAGCTGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACAAGCGCCGCTgtg                                                                                                                                      >  3:42690/4‑147 (MQ=255)
                     tGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCAc                                                                                                                               >  6:118791/1‑149 (MQ=255)
                             aTCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGt                                                                                                                        >  3:301398/1‑148 (MQ=255)
                             aTCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGACAAGGCGCAGCGGGAAGTTATTCAGCCGCTCTTGAAGATTGATGCTGCTGACGCTGCCGAGCTTGTCGATATCCACAAGCGCCTCTGGGCCGGCACCTGAGGt                                                                                                                        >  1:208668/1‑148 (MQ=255)
                               cAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGAt                                                                                                                     >  4:18638/1‑149 (MQ=255)
                               cAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGAt                                                                                                                     >  4:22866/1‑149 (MQ=255)
                                aaCTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCt                                                                                                                                         <  2:184963/128‑1 (MQ=255)
                                aaCTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCt                                                                                                                                         >  1:184963/1‑128 (MQ=255)
                                aaCTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGAt                                                                                                                     <  2:151686/148‑1 (MQ=255)
                                     ccGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCt                                                                                                                <  4:245378/148‑1 (MQ=255)
                                      cGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTg                                                                                                               <  4:301398/148‑1 (MQ=255)
                                           aTGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTAc                                                                                                         >  5:90211/1‑149 (MQ=255)
                                             gCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGa                                                                                                       >  5:89462/1‑149 (MQ=255)
                                                        ccGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAgg                                                                                            <  3:22866/149‑1 (MQ=255)
                                                        ccGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAgg                                                                                            <  3:18638/149‑1 (MQ=255)
                                                                 gcACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAg                                                                                                   >  5:15131/1‑133 (MQ=255)
                                                                 gcACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAg                                                                                                   <  6:15131/133‑1 (MQ=255)
                                                                   acaTCGCCAAGGCGCAGCGGGAAGTTATACAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACg                                                                                 >  1:179059/1‑149 (MQ=255)
                                                                     aTCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCg                                                                                                             >  2:188627/1‑119 (MQ=255)
                                                                     aTCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCg                                                                                                             <  1:188627/119‑1 (MQ=255)
                                                                      tCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACgctg                                                                              <  6:133843/149‑1 (MQ=255)
                                                                                   gaggtaaGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCGGCTGACGCGCTTGTCGATATCCTCCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGATGCTCTTTGGTCAGc                                                                 <  2:208668/144‑1 (MQ=255)
                                                                                             aTTCCGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCAcgc                                                        <  8:58486/148‑1 (MQ=255)
                                                                                                 aGCCGCTCGTGTAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTc                                                                                       >  3:228778/1‑113 (MQ=255)
                                                                                                 aGCCGCTCGTGTAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTc                                                                                       <  4:228778/113‑1 (MQ=255)
                                                                                                 aGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCTGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATc                                                   <  1:60009/149‑1 (MQ=255)
                                                                                                  gCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCg                                                  >  7:50153/1‑149 (MQ=255)
                                                                                                  gCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCg                                                  <  6:90211/149‑1 (MQ=255)
                                                                                                         gTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCt                                           >  1:47166/1‑149 (MQ=255)
                                                                                                         gTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCt                                           >  7:182098/1‑149 (MQ=255)
                                                                                                            cAGTTTGAAGCTGCTGCCGCTGCCGCGCGTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCtt                                          <  3:180433/147‑1 (MQ=255)
                                                                                                             aGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTtctc                                       >  5:326747/1‑149 (MQ=255)
                                                                                                              gTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTcc                                      >  2:118525/1‑149 (MQ=255)
                                                                                                                  gATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCttt                                   <  5:118791/148‑1 (MQ=255)
                                                                                                                   aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGctc                                                                           <  2:222254/107‑1 (MQ=255)
                                                                                                                   aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGctc                                                                           >  1:222254/1‑107 (MQ=255)
                                                                                                                   aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTAc                                 >  5:156613/1‑149 (MQ=255)
                                                                                                                   aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTAc                                 <  1:118525/149‑1 (MQ=255)
                                                                                                                   aTGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACACTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTAc                                 <  6:89462/149‑1 (MQ=255)
                                                                                                                               ctgccgcGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATa                     <  6:156613/149‑1 (MQ=255)
                                                                                                                                tgccgcGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAg                    >  6:152536/1‑149 (MQ=255)
                                                                                                                                agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg                          <  1:11491/139‑1 (MQ=255)
                                                                                                                                agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg                          <  1:6398/139‑1 (MQ=255)
                                                                                                                                agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg                          >  2:11491/5‑143 (MQ=255)
                                                                                                                                agcggcgcTGGTGGATATCGACAAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCg                          >  2:6398/5‑143 (MQ=255)
                                                                                                                                          gTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGc          >  5:246173/1‑149 (MQ=255)
                                                                                                                                          gTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGc          >  2:201663/1‑149 (MQ=255)
                                                                                                                                             gATATCCACCACCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGctct       >  8:11680/1‑149 (MQ=255)
                                                                                                                                              atatCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGCTCtt      <  1:162328/149‑1 (MQ=255)
                                                                                                                                              atatCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGCTCtt      >  2:160835/1‑149 (MQ=255)
                                                                                                                                              atatCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGCTCtt      <  2:179059/149‑1 (MQ=255)
                                                                                                                                                  ccaccaGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGGCGGCATAGGCGCGTGGGCTCTTGCCa  <  8:50153/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCAGAGAAAGGAGGACCCGTTGCTTCAGATCAACTTCCGGAGATGCATTGCGTTGCCGCAGTGCGCACATCGCCAAGGCGCAGCGGGAAGTTATTCAGCCGCTCGTGCAGTTTGATGCTGCTGCCGCTGCCGCGCTTGTCGATATCCACCAGCGCCGCTGGGCCGGCACCTGAGGTGATTTTCTGCGGTACGATCAGCCGCAGGCCGTCGCCACGCTGCTCTTCGGTCAGCGGGTCACGCGTATCGGCAAGCTTCTCCTTTACGCAGTCGGCATAGGCGCGTGGGCTCTTGCCA  >  NC_002947/4351794‑4352088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑