Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,353,888 C→T 7.7% H338H (CAC→CAT)  yrpB → 2‑nitropropane dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,353,8880CT 7.7% 148.1 / 2.6 52H338H (CAC→CAT) yrpB2‑nitropropane dioxygenase
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base T (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

CGCGAGCTGGGGCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTATCGCTATATAGTTAAGGCTATAACGATAACCTC  >  NC_002947/4353742‑4354028
                                                                                                                                                  |                                                                                                                                            
cgcgAGCTGGGGCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACAt                                                                                                                                            <  4:237359/149‑1 (MQ=255)
 gcgAGCTGGGGCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATg                                                                                                                                           >  8:216423/1‑149 (MQ=255)
 gcgAGCTGGGGCCGATGAGCGAGCTTGAACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATg                                                                                                                                           >  3:154906/1‑149 (MQ=255)
     gCTGGGGCCGATGAGCGAGCTTGCACCGCTCTTGCCGCTGGCGGGCGTGGCATTGATGCATTTTCGGGCGATCACCGATCCGCAGGGTAAGATTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGcc                                                                                                                                         <  4:154906/147‑1 (MQ=255)
        ggggCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAAGCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCcggcgg                                                                                                                                    <  5:256533/149‑1 (MQ=255)
         gggCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCATGTGCcggccta                                                                                                                                   <  4:138540/149‑4 (MQ=255)
         gggCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCcggcgg                                                                                                                                    <  2:162819/148‑1 (MQ=255)
          ggCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGt                                                                                                                                  <  4:39574/149‑1 (MQ=255)
           gCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGTATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTg                                                                                                                                 <  3:279955/149‑1 (MQ=255)
              gATGAGCGTGCTTGCACCGCGCTTCCCGCTGGCGGGCGTGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCTGCAATGTGTGGTCGGTGCAGGCGTTACGGCTAGGCCGGCACATGCGGGCGGGTGAGc                                                                                                                              <  4:257874/149‑1 (MQ=255)
              gATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGc                                                                                                                              >  8:97683/1‑149 (MQ=255)
              gATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGc                                                                                                                              >  5:137291/1‑149 (MQ=255)
                        cTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGga                                                                                                                    >  8:296386/1‑149 (MQ=255)
                            cACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCATGTGCcggccta                                                                                                                                   <  5:123892/130‑4 (MQ=255)
                            cACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCATGTGCcggccta                                                                                                                                   >  6:123892/1‑127 (MQ=255)
                              ccGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGc                                                                                                              <  8:47033/149‑1 (MQ=255)
                                 cgcTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCgg                                                                                                           >  4:231869/1‑149 (MQ=255)
                                    ttCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGa                                                                                                                                >  4:50588/1‑125 (MQ=255)
                                     tCCCGCGGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAg                                                                                                       <  8:245157/149‑1 (MQ=255)
                                         gCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCcggcgg                                                                                                                                    <  7:142959/116‑1 (MQ=255)
                                         gCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCcggcgg                                                                                                                                    >  8:142959/1‑116 (MQ=255)
                                             gcgggcggGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTgg                                                                                                <  7:216423/148‑1 (MQ=255)
                                              cgggcgggGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCa                                                                                              <  7:97683/149‑1 (MQ=255)
                                               gggcgggGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAg                                                                                             <  6:12401/149‑1 (MQ=255)
                                                ggcgggGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGt                                                                                            <  6:137291/149‑1 (MQ=255)
                                                    gggCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGATAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATc                                                                                        <  2:206/149‑1 (MQ=255)
                                                           gATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTaa                                                                                                        <  5:107109/126‑1 (MQ=255)
                                                           gATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTaa                                                                                                        >  6:107109/1‑126 (MQ=255)
                                                            aTGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCa                                                                                              <  2:93589/135‑1 (MQ=255)
                                                            aTGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCa                                                                                              >  1:93589/1‑135 (MQ=255)
                                                            aTGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCAcc                                                                                 <  3:231869/148‑1 (MQ=255)
                                                                      gggCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGa                                                                      >  2:121317/1‑149 (MQ=255)
                                                                        gCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGcc                                                                                                             <  4:273199/108‑1 (MQ=255)
                                                                        gCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGcc                                                                                                             >  3:273199/1‑108 (MQ=255)
                                                                              aCCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGAcaggcagg                                                              >  6:173679/1‑149 (MQ=255)
                                                                                cGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAggcaggca                                                            >  2:280628/1‑149 (MQ=255)
                                                                                  aTCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATg                                                          <  7:296386/149‑1 (MQ=255)
                                                                                  aTCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATg                                                          <  8:156686/149‑1 (MQ=255)
                                                                                      gCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGc                                                        <  7:242780/147‑1 (MQ=255)
                                                                                      gCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGc                                                        >  8:242780/1‑147 (MQ=255)
                                                                                                   gATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACATGCAGGCATGGCCTCTTCACGGCCAAt                                         >  4:198877/1‑149 (MQ=255)
                                                                                                          gCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTAt                                  >  1:296441/1‑149 (MQ=255)
                                                                                                          gCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTAt                                  >  8:13660/1‑149 (MQ=255)
                                                                                                             aTCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTgg                                                                                                >  5:154934/1‑84 (MQ=255)
                                                                                                             aTCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTgg                                                                                                <  6:154934/84‑1 (MQ=255)
                                                                                                                    gTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCa                                                 >  3:179499/1‑124 (MQ=255)
                                                                                                                    gTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCa                                                 <  4:179499/124‑1 (MQ=255)
                                                                                                                        gggCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTATCGCTATATAGTTaa                    <  1:280628/149‑1 (MQ=255)
                                                                                                                          gCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTATCGCTATATAGTTAAgg                  <  1:121317/149‑1 (MQ=255)
                                                                                                                                 ttACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTATCGCTATATAGTTAAGGCTATAAc           >  8:249345/1‑149 (MQ=255)
                                                                                                                                 ttACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTATCGCTATATAGTTAAGGCTATAAc           >  4:188884/1‑149 (MQ=255)
                                                                                                                                            ccGGCATGTGCCGGCGGGTGAGCTGACCCGGCAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTATCGCTATATAGTTAAGGCTATAACGATAACCTc  >  6:132732/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CGCGAGCTGGGGCCGATGAGCGAGCTTGCACCGCGCTTCCCGCTGGCGGGCGGGGCATTGATGCCTTTACGGGCGATCACCGATCCGCAGGGTAAGAGTGATTTCAGCAATCTGTGGTCGGGGCAGGCGTTACGGCTAGGCCGGCACATGCCGGCGGGTGAGCTGACCCGGGAGATTGCCGGTAAGGCATTGGCAGTGATCGGTCACCAGGCATTCTGACAGGCAGGCATGGCCTCTTCACGGCCAATGGCTTATCGCTATATAGTTAAGGCTATAACGATAACCTC  >  NC_002947/4353742‑4354028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑