Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 358,091 G→A 15.3% intergenic (+59/‑71) tdcG‑I → / → PP_5441 L‑serine dehydratase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947358,0910GA15.3% 96.6 / 7.9 46intergenic (+59/‑71)tdcG‑I/PP_5441L‑serine dehydratase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/13);  new base A (2/4);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

CGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGGT  >  NC_002947/357952‑358238
                                                                                                                                           |                                                                                                                                                   
cgcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCt                                                                                                                                             <  7:161917/148‑1 (MQ=255)
cgcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTg                                                                                                                                            <  7:19392/149‑1 (MQ=255)
     tACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGa                                                                                                                                       <  8:72467/149‑1 (MQ=37)
       ccGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAg                                                                                                                                      <  6:149257/148‑1 (MQ=255)
                    gCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGt                                                                                                                                           >  8:94969/1‑130 (MQ=255)
                             gTACAACGCCACCTCGCGCGGCGGCCTGGCGGTCCGCGCTAGCGCGTGCTGATTTTCTGCGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTCCAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGccc                                                                                                               <  6:273919/149‑1 (MQ=17)
                             gTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCa                                                                                                                                                   <  4:269530/113‑1 (MQ=255)
                             gTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCa                                                                                                                                                   >  3:269530/1‑113 (MQ=255)
                              tACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCgg                                                                                                                                   >  6:308691/1‑128 (MQ=255)
                              tACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCgg                                                                                                                                   <  5:308691/128‑1 (MQ=255)
                                 aaaGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGACTGTGCAATCCCTGGTGGAGCGGGGTTGCCCGGGAAGAGGGCCtt                                                                                                             >  1:109299/1‑147 (MQ=38)
                                 aaaGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCtt                                                                                                             >  1:55643/1‑147 (MQ=255)
                                  aaGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATcc                                                                                                                                               <  1:95653/112‑1 (MQ=255)
                                  aaGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATcc                                                                                                                                               >  2:95653/1‑112 (MQ=255)
                                   cgccaCCCCGCGCGCCGGCCGGGCGGCCCCCGCTACCGCGCGCTGATTTCCGGCCCTCACCTTCTGGCCCTCTCCCCGGGCACGCCCGCCCCACCAGCACCTGTCCAATCCCTGACGGCGCGGGCTTGCCCGCGAAGAGGCCCTTGAgg                                                                                                         <  8:274526/145‑1 (MQ=255)
                                    ccgatcgcgcgcgGCGGCAGGGCGGTCACCGCTTTCCAGTGCTGATTTCCTGTGCTCACCTTCTGGCCCTCTTCGCGGGCAAGCCCGCCCCTACAGCAACTGTCCAACCCCTGTCGCAGCGGGCTTGCCCGCGAAGAGGCCCTTGAg                                                                                                          <  4:269932/140‑1 (MQ=21)
                                          tCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCtt                                                                                                                               <  2:150157/120‑1 (MQ=255)
                                          tCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCtt                                                                                                                               >  1:150157/1‑120 (MQ=255)
                                            gcgcgGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCGGTAGGAGCGGGCTTGCCCGCGGAGAGGCCCGTGAg                                                                                                          >  3:269932/1‑139 (MQ=255)
                                                                    tATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAACCCCCCAACAACACGAACAGTGCAAAACCAGTACGACCGGCGGTGCCCGAGAACACGCCCATGAGGCGGATGCAGGGTCCACACCAACGccgaatccg                                                                         >  4:221359/1‑141 (MQ=255)
                                                                         agggCTGATTTTCTGAGCGCCCCTTCTGGGCCGCTCCGCGGGCAAGCCCGCTCCTCCCGCAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTAt                                                                   <  5:183410/146‑1 (MQ=37)
                                                                           tGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATgg                                                                 >  3:37552/1‑149 (MQ=255)
                                                                             cTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGTACTGTGCAATCCCAGTAGGACCGGGCTTTCGCGCGCAGAGGCCCGTGAGGCTGGAGGCGGTGGAACACCACCGCCTATGCCTCTTTATCGTg                                                               >  6:237384/1‑149 (MQ=25)
                                                                              tGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGc                                                              >  1:246052/1‑149 (MQ=255)
                                                                                aTTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTCCAATCCCTGTCGGTGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCg                                                             <  6:173840/148‑1 (MQ=255)
                                                                                 ttttCTGCGCGGACCTGCTGGCCCTCTTCGCGGGCAACCCCGCCCCAACAGCAACTGTCCAACCCCTGTCGCAGCGGGCTCCCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCgg                                                            <  2:109299/148‑1 (MQ=255)
                                                                                 ttttCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGGAATGCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGAAGGTGCAACACAACCGCCTATGCCTCTTTATGGTTCGGt                                                           >  7:53858/1‑149 (MQ=255)
                                                                                             aCCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGcccc                                               >  1:70887/1‑149 (MQ=255)
                                                                                                  acgggCCTCTGCGCGGCCACGCCCGCGCCTCCCGGCTTTGCACCGTCCCTGTCGGTGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTa                                          <  1:114294/147‑1 (MQ=25)
                                                                                                       ccTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATccc                                                                                                                                              <  5:315783/44‑1 (MQ=255)
                                                                                                       ccTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATccc                                                                                                                                              >  6:315783/1‑44 (MQ=255)
                                                                                                           ttCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcgtc                                 >  5:131496/1‑149 (MQ=255)
                                                                                                                gggCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATg                            >  4:236708/1‑149 (MQ=255)
                                                                                                                 ggCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGt                           >  1:73136/1‑149 (MQ=255)
                                                                                                                       cccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGTTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt                     >  1:289590/1‑149 (MQ=255)
                                                                                                                       cccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt                     <  3:236708/149‑1 (MQ=255)
                                                                                                                       ccagCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt                     <  8:53858/146‑1 (MQ=255)
                                                                                                                          gCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGAGGTGATGGGCGCCCCGCCTACCGATCCTCGGATGTCGCGATCac                  >  7:250117/1‑148 (MQ=255)
                                                                                                                            tCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGAt                             <  5:257058/136‑1 (MQ=37)
                                                                                                                            tCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGAt                             >  6:257058/1‑136 (MQ=37)
                                                                                                                              cTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGtgtg              >  2:281027/1‑149 (MQ=37)
                                                                                                                               tACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTgg             >  4:304407/1‑149 (MQ=255)
                                                                                                                               tACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTgg             >  3:158130/1‑149 (MQ=255)
                                                                                                                                 ccgctaCTGTGCAATCCCTGTCGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTgg             <  5:237384/142‑1 (MQ=255)
                                                                                                                                 cAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCg                                                                                  >  6:255103/1‑78 (MQ=255)
                                                                                                                                 cAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCg                                                                                  <  5:255103/78‑1 (MQ=255)
                                                                                                                                          tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGc           <  6:244042/140‑1 (MQ=255)
                                                                                                                                          tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGc           >  5:244042/1‑140 (MQ=255)
                                                                                                                                          tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGGt  >  6:151134/1‑149 (MQ=255)
                                                                                                                                          tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGGt  <  5:151134/149‑1 (MQ=255)
                                                                                                                                          tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGGCGGt  >  8:290553/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGGT  >  NC_002947/357952‑358238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑