| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,410,778 | G→C | 11.1% | H121Q (CAC→CAG) | PP_3894 ← | replication protein O |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,410,778 | 0 | G | C | 11.1% | 128.6 / 7.2 | 45 | H121Q (CAC→CAG) | PP_3894 | replication protein O |
| Reads supporting (aligned to +/- strand): ref base G (22/18); new base C (3/2); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
AGGGGTTGCCTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCG > NC_002947/4410631‑4410925 | aGGGGTTGCCTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAgt < 6:271220/149‑1 (MQ=255) ggggTTGCCTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAgtg > 6:263870/1‑149 (MQ=255) gTTGCCTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAgtgtgt > 1:19223/1‑149 (MQ=255) ccTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGa < 2:223771/148‑1 (MQ=255) tCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCa > 8:284496/1‑148 (MQ=255) cGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCaa > 6:108367/1‑136 (MQ=255) cGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCaa < 5:108367/136‑1 (MQ=255) cGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTg > 5:256843/1‑149 (MQ=255) cGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTg < 3:137442/149‑1 (MQ=255) ccTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTc < 2:19223/148‑1 (MQ=255) gcCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCaa < 2:318107/123‑1 (MQ=255) gcCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCaa > 1:318107/1‑123 (MQ=255) cGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAg > 3:115932/1‑149 (MQ=255) cGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACACTGTGTAGaaaaca < 1:161006/116‑6 (MQ=255) cGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACACTGTGTAGaaaaca > 2:161006/1‑111 (MQ=255) ggatgcgTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGCTGTTTTCTACACAGTGTGTAGATTTCAACACGGATGGCTTGCTGTCGCTTCTCCACTCAGGTTCCg > 8:117841/1‑149 (MQ=255) gatgcgTCGACAACCAATTCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCgg < 6:256843/149‑1 (MQ=255) tCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCgg < 6:32147/143‑1 (MQ=255) tCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCgg > 5:32147/1‑143 (MQ=255) gACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGaaa < 5:145517/148‑1 (MQ=255) gTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTGAACAGGGGTGTTTTATACACAGTGTGTAGATTTAAACACGGTTGGCTTGATGTCGATTATCCACTCAGGTACCGGTAGTAAAGTGATTGGGTcg > 1:86566/1‑149 (MQ=255) gtatttCTGTCTTATATGTGTGTAATTTTCAACACAGGG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgc < 7:117841/148‑1 (MQ=255) cTGGCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa < 5:263870/148‑1 (MQ=255) tGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa < 8:276365/147‑1 (MQ=255) tGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGaaaa > 7:276365/1‑147 (MQ=255) ttttATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgcg > 8:137079/1‑138 (MQ=255) ttttATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgcg < 7:137079/138‑1 (MQ=255) tgtgtAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCAc > 2:216793/1‑140 (MQ=255) tgtgtAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCAc < 1:216793/140‑1 (MQ=255) aaTTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATc > 3:288873/1‑148 (MQ=255) ttttCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgc > 6:132774/1‑123 (MQ=255) ttttCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgc < 5:132774/123‑1 (MQ=255) acacAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTAGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAAtt > 7:141899/1‑148 (MQ=255) aGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTgg > 3:74989/1‑87 (MQ=255) aGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTgg < 4:74989/87‑1 (MQ=255) tgtTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAATGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAAGTGCGCGGCCgagccgcg > 8:127260/1‑144 (MQ=255) aaatCTACACACTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgcg > 6:219233/4‑101 (MQ=255) aaatCTACACACTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATcgcg < 5:219233/98‑1 (MQ=255) ttCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTTGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCga < 7:181849/139‑1 (MQ=255) ttCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCgaga < 5:216193/141‑1 (MQ=255) ttCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCgaga > 6:216193/1‑141 (MQ=255) ttCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCga < 7:177165/139‑1 (MQ=255) ttCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCga > 8:177165/1‑139 (MQ=255) ttCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCga > 8:181849/1‑139 (MQ=255) atCTACACACTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCTAGACACGTTgg > 3:240840/2‑149 (MQ=255) acaGTGTGTGGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCt > 3:264847/1‑149 (MQ=255) acaGTGTGTAGATTTCAACACGGTTGGATTGCTTTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCt > 4:48542/1‑149 (MQ=255) aGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGAAGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCg > 1:137316/1‑149 (MQ=255) | AGGGGTTGCCTTGTCGTTGCCGTGACCGCCTTCCTCGGCGCCGGTCGATGCGGATGCGTCGACAACCAATGCGTTAGCATTGGTATTTGTATTTCTGTCTTTTATGTGTGTAATTTTCAACACAGTG‑GCAGGGGTGTTTTCTACACAGTGTGTAGATTTCAACACGGTTGGCTTGCTGTCGATTCTCCACTCAGGTACCGGTAGGAAAGTGATTGGGTCGCGACTGCCGCCATCGCGGAAAAGCACGCGCTGACGGATCAACGAATTGATCGCCCGAGACACGTTGGCGCGCTCG > NC_002947/4410631‑4410925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |