Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,509,782 C→G 6.3% A94P (GCC→CCC)  serS ← serine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,509,7820CG 6.3% 202.5 / 3.9 63A94P (GCC→CCC) serSserine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (28/31);  new base G (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

CCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGT  >  NC_002947/4509641‑4509927
                                                                                                                                             |                                                                                                                                                 
ccACCTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATg                                                                                                                                            <  7:203107/149‑1 (MQ=255)
     tGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATTCAGGCCGAGCTGGATGGCATc                                                                                                                                       <  2:202031/149‑1 (MQ=255)
     tGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTc                                                                                                                                       <  5:275852/149‑1 (MQ=255)
       gTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTcc                                                                                                                                      <  7:127964/148‑1 (MQ=255)
        tCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAg                                                                                                                                    <  8:35480/149‑1 (MQ=255)
          cTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCt                                                                                                                                  >  5:227046/1‑149 (MQ=255)
           ttGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCt                                                                                                                                  >  2:115768/1‑148 (MQ=255)
                                ttCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCt                                                                                                                                  <  4:89220/127‑1 (MQ=255)
                                ttCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCt                                                                                                                                  >  3:89220/1‑127 (MQ=255)
                                    gCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGTTCAGCCTTGCCGGCAGCCAGTTCGTTg                                                                                                        <  5:8167/149‑1 (MQ=255)
                                    gCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTg                                                                                                        <  5:135366/149‑1 (MQ=255)
                                    gCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTg                                                                                                        >  8:216176/1‑149 (MQ=255)
                                         ccccAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCAt                                                                                                   >  4:246651/1‑149 (MQ=255)
                                          cccAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATg                                                                                                  >  7:72005/1‑149 (MQ=255)
                                            cAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATgcg                                                                                                >  6:105111/1‑149 (MQ=255)
                                            cAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGACTTGCCGGCAGCCAGTTCGTTGGCCATgcg                                                                                                >  4:159317/1‑149 (MQ=255)
                                                   gcACTTCGACGTTGTCGTCTTCACTGGCGCGGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACg                                                                                         >  1:25218/1‑149 (MQ=255)
                                                     aCTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGt                                                                                        >  4:173581/1‑148 (MQ=255)
                                                       ttCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAg                                                                                     >  6:257941/1‑149 (MQ=255)
                                                       ttCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAg                                                                                     >  4:115171/1‑149 (MQ=255)
                                                       ttCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGATTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAg                                                                                     >  2:159424/1‑149 (MQ=255)
                                                                 tcgtcTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCgg                                                                           >  2:50084/1‑149 (MQ=255)
                                                                       tCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAt                                                                     >  3:162114/1‑149 (MQ=255)
                                                                        cACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTGAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAt                                                                     <  8:242416/148‑1 (MQ=255)
                                                                        cACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAt                                                                     <  5:80863/148‑1 (MQ=255)
                                                                        cACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAt                                                                     <  1:115768/148‑1 (MQ=255)
                                                                         aCTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAt                                                                     >  2:217975/1‑147 (MQ=255)
                                                                         aCTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAt                                                                     <  1:217975/147‑1 (MQ=255)
                                                                           tGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGtt                                                                                                         <  5:81042/109‑1 (MQ=255)
                                                                           tGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGtt                                                                                                         >  6:81042/1‑109 (MQ=255)
                                                                                  gACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAAGACATCGcccc                                                          <  7:107324/149‑2 (MQ=255)
                                                                                    ccGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCccttg                                                        <  4:162114/149‑1 (MQ=255)
                                                                                       gCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCccttggcc                                                     >  4:303381/1‑149 (MQ=255)
                                                                                          cGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCccttggccttg                                                  <  4:249138/149‑1 (MQ=255)
                                                                                           gCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCccttggccttgg                                                 >  3:275192/1‑149 (MQ=255)
                                                                                                    cGGCAGGTTGGGAATGGTCAGCAGGATGACATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTtggcctgg                                            >  6:42390/1‑145 (MQ=255)
                                                                                                     ggCAGATTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGAt                                       <  1:159424/149‑1 (MQ=255)
                                                                                                       cAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATgg                                     <  3:173581/149‑1 (MQ=255)
                                                                                                           ttGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACtt                                 <  1:300318/149‑1 (MQ=255)
                                                                                                             gggAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTg                                <  7:216176/148‑1 (MQ=255)
                                                                                                              ggAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTgg                               <  3:246651/148‑1 (MQ=255)
                                                                                                                aaTGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCAGGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAAc                            >  6:244097/1‑149 (MQ=255)
                                                                                                                   ggTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGAt                                       >  5:162367/1‑135 (MQ=255)
                                                                                                                   ggTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGAt                                       <  6:162367/135‑1 (MQ=255)
                                                                                                                    gTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGc                        <  3:303381/149‑1 (MQ=255)
                                                                                                                     tgagctgGACGCCATTCAGGCCGAGCTGGATGGCATCCTGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTtggcctggcc                                          >  5:283429/3‑130 (MQ=25)
                                                                                                                     tgagctgGACGCCATTCAGGCCGAGCTGGATGGCATCCTGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTtggcctggcc                                          <  6:283429/128‑1 (MQ=25)
                                                                                                                       agcagGATGCCATCCAGCTCGGGAAGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGt                      >  7:197959/1‑148 (MQ=255)
                                                                                                                       agcagGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGt                      >  8:63991/1‑148 (MQ=255)
                                                                                                                        gcagGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTtggcctgg                                            >  5:269666/1‑125 (MQ=255)
                                                                                                                        gcagGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTtggcctgg                                            <  6:269666/125‑1 (MQ=255)
                                                                                                                        gcagGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCAGGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTa                    <  5:244097/149‑1 (MQ=255)
                                                                                                                           ggATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCGTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGc                 <  5:257941/149‑1 (MQ=255)
                                                                                                                              tGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCcctt                                                         <  2:271426/106‑1 (MQ=255)
                                                                                                                              tGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCcctt                                                         >  1:271426/1‑106 (MQ=255)
                                                                                                                              tGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCa              <  6:227046/149‑1 (MQ=255)
                                                                                                                                 cATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGACATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAATGGGCGTTACGCTCAGcc           >  2:181756/1‑149 (MQ=255)
                                                                                                                                    ccAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTg         >  6:95337/1‑148 (MQ=255)
                                                                                                                                     cAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCa       <  8:197959/149‑1 (MQ=255)
                                                                                                                                     cAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCa       <  1:50084/149‑1 (MQ=255)
                                                                                                                                     cAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCGTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCa       <  8:72005/149‑1 (MQ=255)
                                                                                                                                         tCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAgttg   >  3:97841/1‑149 (MQ=255)
                                                                                                                                           ggCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAgttgt  <  2:25218/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGT  >  NC_002947/4509641‑4509927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑