Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,509,935	G→A	6.3%	R43C (CGC→TGC)	serS ←	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,509,935	0	G	A	6.3%	190.6 / 2.2	63	R43C (CGC→TGC)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (28/31);  new base A (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGCATATCGTTCTCTCGTTATATG  >  NC_002947/4509790‑4510080                                                                                                                                                   |                                                                                                                                                   gCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGt                                                                                                                                                 <  5:105111/149‑1 (MQ=255)      cAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCGCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTg                                                                                                                                              >  1:237249/1‑148 (MQ=255)         cgcAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGGCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCg                                                                                                                                         <  8:134985/147‑1 (MQ=255)            aGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGcc                                                                                                                                       <  2:237249/148‑1 (MQ=255)                    cGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGgcgc                                                                                                                              >  7:163551/1‑149 (MQ=255)                    cGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTCGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGgcgc                                                                                                                              <  5:42390/149‑1 (MQ=255)                           cAGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCa                                                                                                                       <  5:95337/149‑1 (MQ=255)                            aGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAg                                                                                                                      <  6:320307/149‑1 (MQ=255)                            aGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAg                                                                                                                      >  5:320307/1‑149 (MQ=255)                             gTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGt                                                                                                                     >  1:16708/1‑149 (MQ=255)                                 gTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGAtt                                                                                                                 <  4:97841/149‑1 (MQ=255)                                 gTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGAtt                                                                                                                 <  3:115171/149‑1 (MQ=255)                                  ttGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCAGACCCGCACCgaacaa                                                                                                                                    <  8:282869/129‑6 (MQ=255)                                  ttGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCAGACCCGCACCgaacaa                                                                                                                                    >  7:282869/1‑124 (MQ=255)                                        aTGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATgcg                                                                                                          >  2:266554/1‑149 (MQ=255)                                             cTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGa                                                                                                     <  7:63991/149‑1 (MQ=255)                                             cTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGa                                                                                                     <  4:275192/149‑1 (MQ=255)                                                    tCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCAGACCCGCACCga                                                                                                                                        <  2:286457/107‑2 (MQ=255)                                                    tCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCAGACCCGCACCga                                                                                                                                        >  1:286457/1‑106 (MQ=255)                                                      aGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGc                                                                                           <  8:153153/149‑1 (MQ=255)                                                        c‑cATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGTACCGCCTTGCGGCGCTCTTCCAGTGATTCGAt                                                                                                             >  1:186360/1‑130 (MQ=255)                                                        c‑cATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGTACCGCCTTGCGGCGCTCTTCCAGTGATTCGAt                                                                                                             <  2:186360/130‑1 (MQ=255)                                                        c‑cATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTg                                                                                         <  3:159317/149‑1 (MQ=255)                                                                cGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAg                                                                                             >  3:298584/1‑138 (MQ=255)                                                                cGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAg                                                                                             <  4:298584/138‑1 (MQ=255)                                                                  gggCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACgg                                                                                <  1:266554/149‑1 (MQ=255)                                                                   ggCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACgg                                                                                >  6:172854/1‑148 (MQ=255)                                                                         gTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGc                                                                           >  7:197236/1‑147 (MQ=255)                                                                            ttCGNCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGc                                                                      >  5:32137/1‑149 (MQ=255)                                                                            ttCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTc                                                                                                                         >  4:247578/1‑99 (MQ=255)                                                                            ttCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTc                                                                                                                         <  3:247578/99‑1 (MQ=255)                                                                            ttCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTg                                                                                                                    >  3:6758/1‑104 (MQ=255)                                                                            ttCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTg                                                                                                                    <  4:6758/104‑1 (MQ=255)                                                                            ttCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGc                                                                      >  1:15134/1‑149 (MQ=255)                                                                            ttCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGc                                                                      >  7:123678/1‑149 (MQ=255)                                                                                 ccttggccttggCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGct                                                                                                                             >  2:246322/1‑90 (MQ=255)                                                                                 ccttggccttggCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGct                                                                                                                             <  1:246322/90‑1 (MQ=255)                                                                                 ccttggccttggCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGAtt                                                                                                                 >  5:268955/1‑102 (MQ=255)                                                                                 ccttggccttggCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGAtt                                                                                                                 <  6:268955/102‑1 (MQ=255)                                                                                 ccttggccttggCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTcc                                                                 <  2:15134/149‑1 (MQ=255)                                                                                 ccttggccttggCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTcc                                                                 <  8:123678/149‑1 (MQ=255)                                                                                   ttggccttggccTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGa                                                                              <  5:72777/134‑1 (MQ=255)                                                                                   ttggccttggccTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGa                                                                              >  6:72777/1‑134 (MQ=255)                                                                                     ggccttggcctGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGc                                                               <  8:197236/147‑1 (MQ=255)                                                                                           ggcctggccGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCCCTTCCt                                                       >  1:262255/1‑149 (MQ=255)                                                                                                 gccGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAg                                                                             >  4:15310/1‑121 (MQ=255)                                                                                                 gccGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAg                                                                             <  3:15310/121‑1 (MQ=255)                                                                                                     aTGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGcc                                             >  4:34984/1‑149 (MQ=255)                                                                                                           ttGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTaa                                       <  3:34984/149‑1 (MQ=255)                                                                                                           ttGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTaa                                       >  6:92578/1‑149 (MQ=255)                                                                                                             ggAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTaa                                       >  2:200110/1‑147 (MQ=255)                                                                                                                 cGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGttt                                 <  6:32137/149‑1 (MQ=255)                                                                                                                 cGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGttt                                 <  8:163551/149‑1 (MQ=255)                                                                                                                    gCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTgg                               >  4:39081/1‑148 (MQ=255)                                                                                                                           gCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAg                        >  5:22828/1‑148 (MQ=255)                                                                                                                           gCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGc                       <  1:200110/149‑1 (MQ=255)                                                                                                                             tCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGCa                      >  7:24265/1‑148 (MQ=255)                                                                                                                                gCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGCATATc                  <  5:172854/149‑1 (MQ=255)                                                                                                                                  cTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGCCCAGG‑TGTCCGCCACTTCCTGAAGTTGGCAGCGTAACAGTTTGGAATCGAGCATATCttt               >  2:284588/1‑149 (MQ=255)                                                                                                                                   tGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGCATATCGt                >  3:107969/1‑148 (MQ=255)                                                                                                                                     cAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGCATATCGTtct             <  8:24265/149‑1 (MQ=255)                                                                                                                                           ttCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGttt                                 >  3:120152/1‑123 (MQ=255)                                                                                                                                           ttCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGttt                                 <  4:120152/123‑1 (MQ=255)                                                                                                                                                   gggTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACATGCCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGCATATCGTTCTCTCGTTATATg  >  1:78984/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGC‑CATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCTCAGCCTGCAGTTGTTCGGTGCGGGTCTGCACCGCCTTGCGGCGCTCTTCCAGTGATTCGATGCGCGCGACAT‑CCAGGCTGAAGCCACGGGAGGCCAGGCGGTCCGCCACTTCCTGAAGTTGGCCGCGTAACAGTTTGGAATCGAGCATATCGTTCTCTCGTTATATG  >  NC_002947/4509790‑4510080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑