Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,517,232 A→C 9.3% intergenic (+69/+17) infA → / ← clpA translation initiation factor IF‑1/ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,517,2320AC 9.3% 120.7 / 4.8 43intergenic (+69/+17)infA/clpAtranslation initiation factor IF‑1/ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base A (21/18);  new base C (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TCACTGGCGACAAGGTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGCCAGCGGCCGCTTGA  >  NC_002947/4517087‑4517373
                                                                                                                                                 |                                                                                                                                             
tcACTGGCGACAAGGTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAgg                                                                                                                                            >  3:72180/1‑149 (MQ=255)
  aCTGGCGACAAGGTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAgg                                                                                                                                            <  8:259863/147‑1 (MQ=255)
  aCTGGCGACAAGGTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAgg                                                                                                                                            <  8:253395/147‑1 (MQ=255)
       cGACAAGGTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGcccc                                                                                                                                     <  5:15735/149‑1 (MQ=255)
        gACAAGGTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCt                                                                                                                                    <  8:288151/149‑1 (MQ=255)
              gTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACAt                                                                                                                              >  7:18595/1‑149 (MQ=255)
                               gCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGt                                                                                                             <  2:41388/149‑1 (MQ=255)
                                  gTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCCTTGCAGCCCCTGc                                                                                                                                  >  4:202684/1‑125 (MQ=255)
                                  gTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCCTTGCAGCCCCTGc                                                                                                                                  <  3:202684/125‑1 (MQ=255)
                                  gTACGACCTGAGCAAGGGACGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGt                                                                                                          >  7:131822/1‑149 (MQ=255)
                                          tGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGt                                                                                                  >  4:301259/1‑149 (MQ=255)
                                           gAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTc                                                                                                 >  1:49063/1‑149 (MQ=255)
                                            aGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTc                                                                                                 <  8:18595/148‑1 (MQ=255)
                                                     cGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCAGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGt                                                                                       >  8:231894/1‑149 (MQ=255)
                                                      gCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGaa                                                                                                    <  2:294110/135‑1 (MQ=255)
                                                      gCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGaa                                                                                                    >  1:294110/1‑135 (MQ=255)
                                                           aCCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCa                                                                                 >  3:36596/1‑149 (MQ=255)
                                                                 cGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCgcgc                                                                           <  4:72180/149‑1 (MQ=255)
                                                                      gcgcTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTc                                                                      >  1:313090/1‑149 (MQ=255)
                                                                          tAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACa                                                                  >  5:280433/1‑149 (MQ=255)
                                                                           aaGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACAt                                                                 >  3:148903/1‑149 (MQ=255)
                                                                            aGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACAt                                                                 <  8:131822/148‑1 (MQ=255)
                                                                                 aaGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGcacca                                                           <  7:142677/149‑1 (MQ=255)
                                                                                     cATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGcc                                                       >  5:213640/1‑149 (MQ=255)
                                                                                      aTGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGc                                                               >  5:184332/1‑140 (MQ=255)
                                                                                      aTGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGc                                                               <  6:184332/140‑1 (MQ=255)
                                                                                             aGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCt                                               >  5:256484/1‑149 (MQ=255)
                                                                                              gCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTc                                              <  6:280433/149‑1 (MQ=255)
                                                                                                       ggTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTc                                     <  5:272529/149‑1 (MQ=255)
                                                                                                         tGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGc                                   <  3:301259/149‑1 (MQ=255)
                                                                                                              ggCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCaaa                               >  5:167428/1‑148 (MQ=255)
                                                                                                                 ttttttGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAg                            >  3:244216/1‑148 (MQ=255)
                                                                                                                 ttttttGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAg                            >  4:85494/1‑148 (MQ=255)
                                                                                                                          ccTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATctcctc                   <  4:36596/148‑1 (MQ=255)
                                                                                                                          ccTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCg                  >  7:163163/1‑149 (MQ=255)
                                                                                                                              aaGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAGCACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGc                >  5:222306/1‑147 (MQ=255)
                                                                                                                              aaGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGc                <  3:85494/147‑1 (MQ=255)
                                                                                                                                  aaTTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGCCAGCgg          <  6:213640/149‑1 (MQ=255)
                                                                                                                                       cAGGGGCTGCCTTGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGCCAGCGGcc        >  2:87573/1‑146 (MQ=255)
                                                                                                                                       cAGGGGCTGCCTTGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGCCAGCGGcc        <  1:87573/146‑1 (MQ=255)
                                                                                                                                        aGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCg                  <  2:71690/135‑1 (MQ=255)
                                                                                                                                        aGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGAACTCCTCg                  >  1:71690/1‑135 (MQ=255)
                                                                                                                                          gggCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGCCAGCGGCCGCTTGa  >  1:77628/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TCACTGGCGACAAGGTCCGCGTCGAACTGACGCCGTACGACCTGAGCAAGGGCCGCATCACCTACCGTGCGCGCTAAGCTCAAGCCATGAAAAAGCCCGGCCAGGTGCCGGGCTTTTTTGTGCCTCAAGAAATTGCAGGGGCTGCAAGGCAGCCCCTGCACATCACGCGACCTCAGCCGTGGTTTCGAAGTCGAACACCAGTTCGCCATCGCGCAGGTCGACATGCACCACGCCACCATGCTCGGCCAGCTCGCCAAACAGGATCTCCTCGGCCAGCGGCCGCTTGA  >  NC_002947/4517087‑4517373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑