Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,563,656 G→A 6.3% R69C (CGT→TGT)  vgrG‑III ← type VI secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,563,6560GA 6.3% 189.0 / 2.1 63R69C (CGT→TGT) vgrG‑IIItype VI secretion system protein
Reads supporting (aligned to +/- strand):  ref base G (26/33);  new base A (2/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

CGGCACGGTTTTCTGCTGAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTTGAATGAAACCACT  >  NC_002947/4563513‑4563801
                                                                                                                                                |                                                                                                                                                  
cGGCACGGTTTTCTGCTGAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCa                                                                                                                                                  <  7:13535/146‑1 (MQ=255)
   cACGGTTTTCTGCTGAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTTGG‑CCATGCACATGACGCAGTGGcc                                                                                                                                            >  2:94375/1‑149 (MQ=255)
   cACGGTTTTCTGCTGAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGcc                                                                                                                                            >  1:193690/1‑149 (MQ=255)
    aCGGTTTTCTGCTGAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCg                                                                                                                                           <  2:98128/149‑1 (MQ=255)
           tCTGCTGAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTgc                                                                                                                                     <  3:56680/148‑1 (MQ=255)
               ctgAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCt                                                                                                                                  <  5:210195/147‑1 (MQ=255)
                  aaaGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑aaa                                                                                                                            <  7:166064/149‑1 (MQ=255)
                  aaaGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑aaa                                                                                                                            <  1:288450/149‑1 (MQ=255)
                      aTCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGt                                                                                                                        <  2:193690/149‑1 (MQ=255)
                        ccGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACACAGTGGc                                                                                                                                             >  3:72432/1‑127 (MQ=255)
                        ccGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACACAGTGGc                                                                                                                                             <  4:76700/127‑1 (MQ=255)
                        ccGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACACAGTGGc                                                                                                                                             <  4:72432/127‑1 (MQ=255)
                        ccGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACACAGTGGc                                                                                                                                             >  3:76700/1‑127 (MQ=255)
                         cGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGaa                                                                                                                     <  1:90304/149‑1 (MQ=255)
                          gCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAAt                                                                                                                    <  5:107923/149‑1 (MQ=255)
                           ccAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATa                                                                                                                   <  1:103830/149‑1 (MQ=255)
                                 cGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAg                                                                                                             >  2:90767/1‑149 (MQ=255)
                                 cGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAg                                                                                                             >  2:94567/1‑149 (MQ=255)
                                        aaCCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTc                                                                                                      >  6:222694/1‑149 (MQ=255)
                                               ggggggCCCGGTGCGGCTCGCCCACGGGGTGGTAGGCGGTGCGCAAAAAGCCGGTTTCGCCCTGGCTTAAGGCAATTAATTGGTCCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGAc                                                                                                <  1:94375/146‑1 (MQ=255)
                                                gcgGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGt                                                                                              <  5:142701/149‑1 (MQ=255)
                                                    gCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGACGACGtcgaa                                                                                          >  6:221740/1‑145 (MQ=255)
                                                     ccAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGcc                                                                                          >  8:43880/1‑148 (MQ=255)
                                                        gCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGaaa                                                                                      >  1:158302/1‑149 (MQ=255)
                                                            cGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGa                                                                                  <  5:222694/149‑1 (MQ=255)
                                                             ggCTCGACCACGGCGTGGTAGCGCGTGAGACAAAAGCCGGTTTGGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCTCATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGat                                                                                 <  8:181046/149‑1 (MQ=255)
                                                              gCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGata                                                                                <  3:130296/149‑1 (MQ=255)
                                                                    ccACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACtt                                                                          >  2:57939/1‑149 (MQ=255)
                                                                        ggCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCg                                                                       >  2:100784/1‑148 (MQ=255)
                                                                        ggCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGt                                                                      <  2:158302/149‑1 (MQ=255)
                                                                              gTAGGGCGTGCTAAAAACGCCGGTTTCGCCCTGGCTGACGGAACTGACTAGG‑CCATGCACATGACGCAGTGGCGGGTCTGCGCTGTCTAATGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTc                                                                 <  1:90767/149‑1 (MQ=255)
                                                                              gTAGCGCGTGCTAAAAAAGCCGGTTTCGCCATGGCTGATGGAAATGACTAGG‑CAATGAACATTACGCAGTGGCCGGTCTGCGCTGTCAAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTc                                                                 <  1:94567/149‑1 (MQ=255)
                                                                                 gcgcGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACaa                                                             <  2:56167/149‑1 (MQ=255)
                                                                                     gTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACaa                                                             <  5:126501/145‑1 (MQ=255)
                                                                                     gTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACaa                                                             >  6:126501/1‑145 (MQ=255)
                                                                                      tGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCTCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTcc                                                        <  5:221740/149‑1 (MQ=255)
                                                                                         gACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATAACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAgg                                                     >  6:24933/1‑149 (MQ=255)
                                                                                          aCAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAg                                                      <  5:170997/147‑1 (MQ=255)
                                                                                          aCAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAg                                                      >  6:170997/1‑147 (MQ=255)
                                                                                           cAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAg                                                                                                   >  7:175456/1‑101 (MQ=255)
                                                                                           cAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAg                                                                                                   <  8:175456/101‑1 (MQ=255)
                                                                                            aaaaGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGtt                                                   >  5:139630/1‑148 (MQ=255)
                                                                                              aaGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTTAATAGCGCAGGCTTGTCGAGGACTTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAg                                                <  7:1348/149‑1 (MQ=255)
                                                                                                          cccTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTg                                    >  7:106070/1‑149 (MQ=255)
                                                                                                           ccTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTgg                                   >  6:45602/1‑149 (MQ=255)
                                                                                                               gCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGtt                                 >  6:136479/1‑147 (MQ=255)
                                                                                                                 tGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGca                                              <  7:223118/132‑1 (MQ=255)
                                                                                                                 tGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGca                                              >  8:223118/1‑132 (MQ=255)
                                                                                                                   aaGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAgg                             <  6:139630/147‑1 (MQ=255)
                                                                                                                          tGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGcac                                             <  7:141619/124‑1 (MQ=255)
                                                                                                                          tGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGcac                                             >  8:141619/1‑124 (MQ=255)
                                                                                                                            aCTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCt                                     >  7:271249/1‑130 (MQ=255)
                                                                                                                            aCTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCt                                     <  8:271249/130‑1 (MQ=255)
                                                                                                                                 g‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTtga             <  5:45602/149‑1 (MQ=255)
                                                                                                                                 g‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTtg              >  7:5605/1‑148 (MQ=255)
                                                                                                                                    cATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGaaa                                                                                      <  4:63476/74‑1 (MQ=255)
                                                                                                                                    cATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGaaa                                                                                      >  3:63476/1‑74 (MQ=255)
                                                                                                                                        cacaTGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGTTTAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTTGAATGaaa       <  2:247131/149‑1 (MQ=255)
                                                                                                                                        cacaTGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTTGAATGaaa       <  1:100784/149‑1 (MQ=255)
                                                                                                                                         acaTGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTTGAATGAAAc      >  5:266291/1‑149 (MQ=255)
                                                                                                                                             gACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCGGAAATCGATAGCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTTGAATGAAACCACt  >  5:222966/1‑149 (MQ=255)
                                                                                                                                              aCGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATgg                                       >  3:68178/1‑111 (MQ=255)
                                                                                                                                              aCGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATgg                                       <  4:68178/111‑1 (MQ=255)
                                                                                                                                                gCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAgg                                                     <  7:43966/95‑1 (MQ=255)
                                                                                                                                                gCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAgg                                                     >  8:43966/1‑95 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGGCACGGTTTTCTGCTGAAAGATCCGCCAGTTCGAACGCAACCGCGCGCGGGCCAGCTGCGGCTCGACCACGGCGTGGTAGCGCGTGCGACAAAAGCCGGTTTCGCCCTGGCTGAAGGCACTGACTAGG‑CCATGCACATGACGCAGTGGCCGGTCTGCGCTGT‑AAAGGGTGAATAGCGCAGGCTTGTCGAGGACGTGGCCGAAATCGATATCACTTTCGTAGCTCACAAGTTCCAGGTTCAGCACAAATGGCTGGTTGAGGGTTTCATCCAGCTTGAATGAAACCACT  >  NC_002947/4563513‑4563801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑