Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,576,036	G→C	6.7%	G145R (GGC→CGC)	ybhP‑II →	phosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,576,036	0	G	C	6.7%	195.3 / 4.0	60	G145R (GGC→CGC)	ybhP‑II	phosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base C (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

GCAACGCGGTCTACCCTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCA  >  NC_002947/4575893‑4576181                                                                                                                                                |                                                                                                                                                  gCAACGCGGTCTACCCTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAg                                                                                                                                              >  8:214624/1‑149 (MQ=255)  cAACGCGGTCTACCCTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAgg                                                                                                                                             <  8:99427/149‑1 (MQ=255)            tACCCTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGt                                                                                                                                   >  6:157334/1‑149 (MQ=255)               ccTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGCACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTg                                                                                                                                >  8:141850/1‑149 (MQ=255)               ccTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTg                                                                                                                                >  8:79771/1‑149 (MQ=255)                  gACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGt                                                                                                                             >  6:26973/1‑149 (MQ=255)                   aCGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAg                                                                                                                                                   >  2:91176/1‑126 (MQ=255)                   aCGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAg                                                                                                                                                   <  1:91176/126‑1 (MQ=255)                        caccacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAACGACAGGTGCACGCAGACCGCATGCACCt                                                                                                                       >  5:74128/1‑149 (MQ=255)                         accacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTg                                                                                                                      <  5:26973/149‑1 (MQ=255)                           cacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTc                                                                                                                    >  7:166883/1‑149 (MQ=255)                           cacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTc                                                                                                                    >  7:264876/1‑149 (MQ=255)                           cacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTACACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTc                                                                                                                    >  6:242228/1‑149 (MQ=255)                             cGGCAATGCGTTGCTGTCCATATACCCGATCATCGAGCACCGAAACCTGGACGTGTCTATCACCTGACCCGATCGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTTCCGGGGCAAGGCCATGTGCATGCGGTCTGCGTGCACCTGTCGt                                                                                                                  <  5:182362/149‑1 (MQ=255)                                  aTGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCg                                                                                                                              <  8:266255/132‑1 (MQ=255)                                  aTGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCg                                                                                                                              >  7:266255/1‑132 (MQ=255)                                     cGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAACGACAGGTGCACGCAGACCGCATGCACCTGGCCTTGccccg                                                                                                           <  6:74128/148‑5 (MQ=37)                                      gTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCAc                                                                                                                         >  8:111332/1‑133 (MQ=255)                                      gTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCAc                                                                                                                         <  7:111332/133‑1 (MQ=255)                                      gTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGaaa                                                                                                         <  6:158678/149‑1 (MQ=255)                                      gTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGaaa                                                                                                         <  8:178410/149‑1 (MQ=255)                                      gTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGaaa                                                                                                         <  5:157334/149‑1 (MQ=255)                                              ccAAGTACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCCCCGGCCCCGAACGCCGCGGCCTGTTGCGCGGTGGGCGGGAGGTGCCGGGGCAAGGCCAGGTGCGGGCGGTCTGCGGGCCCCTGTCGTTGCTGGAAGGCCACCgg                                                                                                 >  2:17845/1‑149 (MQ=255)                                               cAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCgg                                                                                                 >  4:251410/1‑148 (MQ=255)                                               cAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGc                                                                                                <  8:166883/149‑1 (MQ=255)                                               cAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCC‑GGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGc                                                                                                >  7:117814/1‑148 (MQ=255)                                                aaaTACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGc                                                                                                >  8:191415/1‑148 (MQ=255)                                                     cccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAag                                                                                           >  2:224159/1‑148 (MQ=255)                                                     cccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAag                                                                                           >  2:228478/1‑148 (MQ=255)                                                      ccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAag                                                                                           >  5:157419/1‑147 (MQ=255)                                                      ccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAag                                                                                           >  4:261876/1‑147 (MQ=255)                                                       cGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCC‑GGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagcagc                                                                                       <  8:117814/149‑1 (MQ=255)                                                        gATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagcagc                                                                                       >  7:266072/1‑149 (MQ=255)                                                             tcGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTACACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAg                                                                                  <  5:242228/149‑1 (MQ=255)                                                               gAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGtt                                                                                <  6:157419/149‑1 (MQ=255)                                                                   aCCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTtgctg                                                                            <  7:214624/149‑1 (MQ=255)                                                                          ccTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAAc                                                                     <  3:251410/149‑1 (MQ=255)                                                                                cGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTgg                                                               <  8:266072/149‑1 (MQ=255)                                                                                 gtgtCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCCTGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGa                                                              >  3:56998/1‑149 (MQ=255)                                                                                        tCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTg                                                       <  1:72407/149‑1 (MQ=255)                                                                                           ccGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCg                                                    <  7:95601/149‑1 (MQ=255)                                                                                                cccGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCaa                                               <  7:79771/149‑1 (MQ=255)                                                                                                       gccgcGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCg                                        >  1:21062/1‑149 (MQ=255)                                                                                                           cgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGa                                    <  7:191415/149‑1 (MQ=255)                                                                                                                tGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatcatc                               >  4:150935/1‑149 (MQ=255)                                                                                                                  ttGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCt                                                                                      >  4:293059/1‑92 (MQ=255)                                                                                                                  ttGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCt                                                                                      <  3:293059/92‑1 (MQ=255)                                                                                                                        tgtgtgCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGcc                                                          >  1:280465/1‑114 (MQ=255)                                                                                                                        tgtgtgCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGcc                                                          <  2:280465/114‑1 (MQ=255)                                                                                                                             gCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGCGATGATCACCggg                         >  5:280835/1‑141 (MQ=255)                                                                                                                             gCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGCGATGATCACCggg                         <  6:280835/142‑2 (MQ=255)                                                                                                                              cTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGAc                      >  7:286035/1‑144 (MQ=255)                                                                                                                              cTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGAc                      <  8:286035/144‑1 (MQ=255)                                                                                                                              cTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCaa                 <  3:150935/149‑1 (MQ=255)                                                                                                                                ggAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAAc                >  4:110290/1‑148 (MQ=255)                                                                                                                                 gAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGa              >  4:3848/1‑149 (MQ=255)                                                                                                                                    gTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTg           <  1:224159/149‑1 (MQ=255)                                                                                                                                    gTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTg           <  1:228478/149‑1 (MQ=255)                                                                                                                                      tttccagCAACGACAGGTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTg                                     <  5:289597/115‑1 (MQ=25)                                                                                                                                      tttccagCAACGACAGGTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTg                                     >  6:289597/7‑121 (MQ=25)                                                                                                                                         ggggCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGaaaa      <  7:141850/149‑1 (MQ=255)                                                                                                                                             cAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCa  <  4:70328/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  GCAACGCGGTCTACCCTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCA  >  NC_002947/4575893‑4576181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑