Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,580,599 C→A 8.5% Q943H (CAG→CAT) ‡ treSB ← bifunctional trehalose synthase B/maltokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,580,5990CA 8.5% 135.7 / 4.1 47Q943H (CAG→CAT) ‡treSBbifunctional trehalose synthase B/maltokinase
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base A (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

AAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGC  >  NC_002947/4580457‑4580745
                                                                                                                                              |                                                                                                                                                  
aaGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtgcttg                                                                                                                                              >  5:85290/1‑149 (MQ=255)
                     gcTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTgg                                                                                                                         <  3:292744/149‑1 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAt                                                                                                                                <  5:263284/138‑1 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAt                                                                                                                                >  6:263284/1‑138 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTg                                                                                                                          >  1:71554/1‑144 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTg                                                                                                                          <  2:71554/144‑1 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGc                                                                                                                       >  3:113656/1‑147 (MQ=255)
                                   cgggcgggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACg                                                                                                           >  3:218421/1‑149 (MQ=255)
                                   cgggcgggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACg                                                                                                           >  5:287935/1‑149 (MQ=255)
                                        gggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGtt                                                                                                      <  6:85290/149‑1 (MQ=255)
                                              aCCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTtgctg                                                                                                  >  1:80681/1‑147 (MQ=255)
                                                cTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCGGGCTCAGGgtg                                                                                                                                     >  7:166582/1‑110 (MQ=255)
                                                cTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAGGgtg                                                                                                                                     <  8:166582/110‑1 (MQ=255)
                                                  tCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAg                                                                                                                                         <  8:274746/104‑1 (MQ=255)
                                                  tCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAg                                                                                                                                         >  7:274746/1‑104 (MQ=255)
                                                                           ccTACACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTg                                                                   >  5:165932/1‑149 (MQ=255)
                                                                            cTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGt                                                                  >  5:276213/1‑149 (MQ=255)
                                                                                accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                                  <  5:61017/129‑1 (MQ=255)
                                                                                accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                                  >  6:61017/1‑129 (MQ=255)
                                                                                 ccacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgt                                                             >  6:242486/1‑149 (MQ=255)
                                                                                    cGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCGCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCGGTTGGTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgtgtg                                                          >  5:87335/1‑149 (MQ=255)
                                                                                     gAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgtgtgt                                                         >  7:118706/1‑149 (MQ=255)
                                                                                      aGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTc                                                        <  2:252863/149‑1 (MQ=255)
                                                                                       gCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTcg                                                       <  4:74960/149‑1 (MQ=255)
                                                                                         aCCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTcgc                                                      >  2:137884/1‑148 (MQ=255)
                                                                                           cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGAt                                                   >  4:223919/1‑149 (MQ=255)
                                                                                           cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGAt                                                   <  4:113656/149‑1 (MQ=255)
                                                                                               cccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTg                                               <  5:242486/149‑1 (MQ=255)
                                                                                                ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCt                                                                    <  5:278534/127‑1 (MQ=255)
                                                                                                ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCt                                                                    >  6:278534/1‑127 (MQ=255)
                                                                                                ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTgg                                              >  8:158986/1‑149 (MQ=255)
                                                                                                    gTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCca                                          <  2:130077/149‑1 (MQ=255)
                                                                                                       cAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcagc                                        <  3:223919/148‑1 (MQ=255)
                                                                                                              ccGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                >  4:258300/1‑149 (MQ=255)
                                                                                                                   tACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGt                           <  1:176628/149‑1 (MQ=255)
                                                                                                                     cacGCATAAGCAGGCCACCCTGAGGCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                         <  2:80681/149‑1 (MQ=255)
                                                                                                                      acGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCGATGGTGTCGCGATGCTGGGGCAGCAGGTCGAGGGCGTgcg                        >  7:218019/1‑147 (MQ=255)
                                                                                                                      acGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCCGAAGGTCGAGGGCGTggg                        >  6:209201/1‑149 (MQ=255)
                                                                                                                         cATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtca                     <  7:158986/149‑1 (MQ=255)
                                                                                                                             aGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGGTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagtt                  <  3:258300/148‑1 (MQ=255)
                                                                                                                                tcgacACCCTGAGCAAGCAGGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                <  7:182000/127‑1 (MQ=255)
                                                                                                                                tcgacACCCTGAGCAAGCAGGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                >  8:182000/5‑131 (MQ=255)
                                                                                                                                aGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagttcagt              >  2:16176/1‑149 (MQ=255)
                                                                                                                                           aGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCtgtg   <  1:137884/149‑1 (MQ=255)
                                                                                                                                           aGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCtgt    <  6:165932/148‑1 (MQ=255)
                                                                                                                                            gCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGc  <  6:287935/149‑1 (MQ=255)
                                                                                                                                            gCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGGCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGc  <  4:218421/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
AAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGC  >  NC_002947/4580457‑4580745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑