Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,580,604 T→G 8.7% K942Q (AAG→CAG)  treSB ← bifunctional trehalose synthase B/maltokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,580,6040TG 8.7% 133.1 / 4.0 46K942Q (AAG→CAG) treSBbifunctional trehalose synthase B/maltokinase
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base G (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

AAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGCACA  >  NC_002947/4580457‑4580748
                                                                                                                                                   |                                                                                                                                                
aaGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtgcttg                                                                                                                                                 >  5:85290/1‑149 (MQ=255)
                     gcTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTgg                                                                                                                            <  3:292744/149‑1 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAt                                                                                                                                   <  5:263284/138‑1 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAt                                                                                                                                   >  6:263284/1‑138 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTg                                                                                                                             >  1:71554/1‑144 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTg                                                                                                                             <  2:71554/144‑1 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGc                                                                                                                          >  3:113656/1‑147 (MQ=255)
                                   cgggcgggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACg                                                                                                              >  3:218421/1‑149 (MQ=255)
                                   cgggcgggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACg                                                                                                              >  5:287935/1‑149 (MQ=255)
                                        gggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGtt                                                                                                         <  6:85290/149‑1 (MQ=255)
                                              aCCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTtgctg                                                                                                     >  1:80681/1‑147 (MQ=255)
                                                cTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCGGGCTCAGGgtg                                                                                                                                        >  7:166582/1‑110 (MQ=255)
                                                cTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAGGgtg                                                                                                                                        <  8:166582/110‑1 (MQ=255)
                                                  tCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAg                                                                                                                                            <  8:274746/104‑1 (MQ=255)
                                                  tCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAg                                                                                                                                            >  7:274746/1‑104 (MQ=255)
                                                                           ccTACACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTg                                                                      >  5:165932/1‑149 (MQ=255)
                                                                            cTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGt                                                                     >  5:276213/1‑149 (MQ=255)
                                                                                accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                                     >  6:61017/1‑129 (MQ=255)
                                                                                accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                                     <  5:61017/129‑1 (MQ=255)
                                                                                 ccacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgt                                                                >  6:242486/1‑149 (MQ=255)
                                                                                    cGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCGCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCGGTTGGTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgtgtg                                                             >  5:87335/1‑149 (MQ=255)
                                                                                     gAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgtgtgt                                                            >  7:118706/1‑149 (MQ=255)
                                                                                      aGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTc                                                           <  2:252863/149‑1 (MQ=255)
                                                                                       gCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTcg                                                          <  4:74960/149‑1 (MQ=255)
                                                                                         aCCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTcgc                                                         >  2:137884/1‑148 (MQ=255)
                                                                                           cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGAt                                                      >  4:223919/1‑149 (MQ=255)
                                                                                           cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGAt                                                      <  4:113656/149‑1 (MQ=255)
                                                                                               cccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTg                                                  <  5:242486/149‑1 (MQ=255)
                                                                                                ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCt                                                                       >  6:278534/1‑127 (MQ=255)
                                                                                                ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCt                                                                       <  5:278534/127‑1 (MQ=255)
                                                                                                ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTgg                                                 >  8:158986/1‑149 (MQ=255)
                                                                                                    gTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCca                                             <  2:130077/149‑1 (MQ=255)
                                                                                                       cAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcagc                                           <  3:223919/148‑1 (MQ=255)
                                                                                                              ccGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                   >  4:258300/1‑149 (MQ=255)
                                                                                                                   tACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGt                              <  1:176628/149‑1 (MQ=255)
                                                                                                                     cacGCATAAGCAGGCCACCCTGAGGCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                            <  2:80681/149‑1 (MQ=255)
                                                                                                                      acGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCGATGGTGTCGCGATGCTGGGGCAGCAGGTCGAGGGCGTgcg                           >  7:218019/1‑147 (MQ=255)
                                                                                                                      acGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCCGAAGGTCGAGGGCGTggg                           >  6:209201/1‑149 (MQ=255)
                                                                                                                         cATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtca                        <  7:158986/149‑1 (MQ=255)
                                                                                                                             aGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGGTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagtt                     <  3:258300/148‑1 (MQ=255)
                                                                                                                                tcgacACCCTGAGCAAGCAGGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                   <  7:182000/127‑1 (MQ=255)
                                                                                                                                tcgacACCCTGAGCAAGCAGGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                   >  8:182000/5‑131 (MQ=255)
                                                                                                                                aGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagttcagt                 >  2:16176/1‑149 (MQ=255)
                                                                                                                                           aGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCtgtg      <  1:137884/149‑1 (MQ=255)
                                                                                                                                           aGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCtgt       <  6:165932/148‑1 (MQ=255)
                                                                                                                                            gCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGc     <  6:287935/149‑1 (MQ=255)
                                                                                                                                            gCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGGCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGc     <  4:218421/149‑1 (MQ=255)
                                                                                                                                               tgcttgctCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGcaca  >  5:251922/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGCACA  >  NC_002947/4580457‑4580748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑