| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | 100% | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 99.7% | 114.6 / ‑7.3 | 43 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (22/20); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 128.3 / NA | 43 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (23/20); total (23/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499339‑1499618 || cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCtt > 7:50957/1‑149 (MQ=255) gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt < 1:61944/149‑1 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 6:123915/149‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg > 8:23408‑M2/1‑141 (MQ=255) aCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCGCCGCGGGGCATGGCACCCGCGTTGCCGGTGttcgcgggcacg < 2:204907‑M2/149‑13 (MQ=255) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctccc > 1:204907‑M2/1‑128 (MQ=255) ccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAGAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctccc < 1:252945‑M2/149‑22 (MQ=255) gAGGCGGCCATTCAGAGCCGGAACGACGCCTTGCCACCACAGCAGCACTGAGGCTGCCA‑AACGGGGGGCGCGGCCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccacaga > 1:233861‑M2/1‑123 (MQ=255) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcaggcccgctcccacaga > 8:54798‑M2/1‑122 (MQ=255) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccacaga > 1:231011‑M2/1‑122 (MQ=255) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccacagactga > 8:204104‑M2/1‑119 (MQ=255) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCgg < 8:50957/149‑1 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGAATGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCCCGAAAAACGCGCCCCGGTTCATTGCACCGGATTTGCCGGTGTTAGAGGGCA‑CGCCCGCGCCCAC‑ATCCTGAATGCCATTTATCgg > 8:157735/1‑149 (MQ=255) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTAGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCggg > 2:241822/1‑149 (MQ=25) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCCTGCCCCCGAAACAGGCGCCGCCGTGCCTGGCCCCCGCGTTGGCGGTGTTAGCGGGCACCC‑CCCCGCCCAC‑AGCCGGATTGCCATTAACCggg > 7:276185/1‑149 (MQ=255) agcagGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTTCCCGCGAAACAAGCGCCGCCGTGCAAGGCACCCGCTTTGGCCGTGTTAGCGGGCACCC‑CCCCGCCCCC‑CGCCAGAATTCCAATTAGCGGGCCGATCGCGAAAGTggccggc > 6:283306/1‑143 (MQ=255) tGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt > 1:95204/1‑149 (MQ=21) ggAGGCTGCCT‑ATCGGTGGGTGCGGGGGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt < 7:157735/147‑1 (MQ=21) aGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACTTGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 1:237935/148‑1 (MQ=21) ggCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 2:252945/149‑1 (MQ=21) ggCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 5:283306/149‑1 (MQ=21) a‑aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCGGTGCATGGCACCGGCGTTGCCCGTGTTAGCGGGCC‑CGCCCCCTCCCAC‑AGACTGATTTCCATTAAGCCGGCCGATCGCTACAGTGACGGCCCGGTTTTTGTTTCCTccc > 6:246906/1‑147 (MQ=18) tGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGa < 5:246906/149‑1 (MQ=21) gggAGCGGGCGTGCCCGGTAAACAGGCGCGGCTGTGCTTGGCACCGGCTTTGCCGTTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa < 5:191783/149‑1 (MQ=25) ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 3:284854/149‑1 (MQ=25) taGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 8:19517/148‑1 (MQ=25) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 3:271784/1‑149 (MQ=25) gcgggcggGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTTTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 7:121726/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 7:71391/149‑1 (MQ=25) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGc > 6:242733/1‑99 (MQ=14) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGc > 6:238461/1‑99 (MQ=14) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc < 5:135106/149‑1 (MQ=25) gccccgcGAAACCGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGtgcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttg > 6:173830‑M2/3‑47 (MQ=255) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 8:160113/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctccccccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttg < 5:173830‑M2/147‑103 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgt < 6:317540‑M2/147‑104 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgt > 5:317540‑M2/1‑44 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:13180/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:95204/149‑1 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 4:170815/1‑148 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc > 5:308662/1‑149 (MQ=37) ccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 3:170815/149‑1 (MQ=37) gCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGTCCGGTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt > 1:134568/1‑148 (MQ=37) || CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCA‑AACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCAC‑AGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499339‑1499618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |