BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,647,927	G→A	Y265Y (TAC→TAT)	oprB‑II ←	carbohydrate‑selective porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,647,927	0	G	A	100.0%	109.9 / NA	33	Y265Y (TAC→TAT)	oprB‑II	carbohydrate‑selective porin
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/13); total (20/13)

TCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTC  >  NC_002947/1647784‑1648067

tCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTgtaatag                                                                                                                                           <  2:23561/147‑1 (MQ=255)

                        tgctgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCa                                                                                                                  >  3:91582/1‑148 (MQ=255)

                           tgGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCAGTACTGTAATAGTAGCCGGCACGATATTACCCTTTCAAGc                                                                                                               >  5:54238/1‑148 (MQ=255)

                              gcgcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTACAAGCCtt                                                                                                            >  5:86867/1‑148 (MQ=255)

                               cgcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGTCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTg                                                                                                           <  5:54732/148‑1 (MQ=255)

                               cgcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGGAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTg                                                                                                           <  1:19396/148‑1 (MQ=255)

                                gcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTgg                                                                                                          <  6:21084/148‑1 (MQ=255)

                                gcCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCGGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTgg                                                                                                          <  4:93403/148‑1 (MQ=255)

                                       cacaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTg                                                                                                   >  4:75201/1‑148 (MQ=255)

                                           aGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGt                                                                                               <  7:25867/148‑1 (MQ=255)

                                                      ctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGt                                                                                     <  4:122869/147‑1 (MQ=255)

                                                       tCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGtt                                                                                    >  5:3025/1‑147 (MQ=255)

                                                                 gcGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCAtt                                                                         >  4:28165/1‑148 (MQ=255)

                                                                   ggTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTAc                                                                       >  6:140839/1‑148 (MQ=255)

                                                                   ggTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTCCTGGCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTAc                                                                       <  7:115772/148‑1 (MQ=255)

                                                                            cgccgcTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATAGTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTg                                                              >  1:45376/1‑148 (MQ=255)

                                                                                     gggCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCCAGCCGTGGAGACGTGGGGTCCATACCAGTTCGACCGGCATTCCCGCACCCTGGGtgccgct                                                     >  7:48140/1‑148 (MQ=255)

                                                                                                   ccGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTg                                        <  3:61633/147‑1 (MQ=255)

                                                                                                    cGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATACTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGaa                                      <  2:45376/148‑1 (MQ=255)

                                                                                                          tGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCAtt                                >  7:23703/1‑148 (MQ=255)

                                                                                                          tGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCAtt                                >  2:189688/1‑148 (MQ=255)

                                                                                                          tGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCAtt                                >  2:186977/1‑148 (MQ=255)

                                                                                                            tCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAAACATTGc                              >  3:165305/1‑148 (MQ=255)

                                                                                                               ttGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCCCCCTGGGTGCCGCTGCCGCTGCGCTTGAAACCATTGCCgg                           >  4:14648/1‑148 (MQ=255)

                                                                                                               ttGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAACCCATTGCCgg                           >  1:70065/1‑148 (MQ=255)

                                                                                                                 gagaACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGAt                         >  2:162712/1‑148 (MQ=255)

                                                                                                                    aaCATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGAtt                        >  1:9624/1‑146 (MQ=255)

                                                                                                                       aTCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAgg                   >  1:22808/1‑148 (MQ=255)

                                                                                                                            gtgCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTg                >  6:100848/1‑146 (MQ=255)

                                                                                                                             tgCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTg                <  4:18384/145‑1 (MQ=255)

                                                                                                                              gCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGaa             <  3:90807/147‑1 (MQ=255)

                                                                                                                              gCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGaa             <  3:75201/147‑1 (MQ=255)

                                                                                                                                      aTTACTGTAATAGTAGCCGGCAAGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGc    <  8:27573/148‑1 (MQ=255)

                                                                                                                                           tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTc  >  2:15537/1‑145 (MQ=255)

TCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTC  >  NC_002947/1647784‑1648067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`