| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 353,067 | A→G | intergenic (+34/+84) | hisF → / ← cbcV | imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 353,067 | 0 | A | G | 96.2% | 74.4 / NA | 26 | intergenic (+34/+84) | hisF/cbcV | imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (15/10); minor base T (0/1); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCC > NC_002947/352930‑353208 | tggccgCCAGCACCGCGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCccccccc > 6:24125/6‑147 (MQ=255) aTGCCAGCGCGGTGCTGGCGGCCATCATCTTCCACTTTGGCGAGTACACAGTGGCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGGTTTGCAGCCCTTCGCTGGCACGCCCGCTc < 7:36252/148‑1 (MQ=255) cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTccc > 5:193228/1‑146 (MQ=255) cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGATGCTTTGCAGCCCTTCGCGGGCACCCCCCCGCCcac > 2:13974/1‑148 (MQ=255) gcgcGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAg < 8:72606/148‑1 (MQ=255) cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCTCCCACAGGTTTGCTGAGATctct > 8:150107/1‑148 (MQ=255) cATCTTGCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACTTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtgtg < 1:67513/148‑1 (MQ=255) tCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTggg < 2:198903/148‑1 (MQ=255) aCTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCgg < 8:136636/147‑1 (MQ=255) cTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGc < 5:27226/148‑1 (MQ=255) cGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGccc > 6:38359/1‑148 (MQ=255) gAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTCCCACCGGTTTGCGGAGATCTCGGTGGGAGCGGGCGTGCCcg > 6:141311/1‑148 (MQ=39) gTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcgcg < 6:76490/148‑1 (MQ=255) acacAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCCCCCGCTACACAACGTTTGCAGAGATATGTGTGGGAGCGGGCGTGCAAGCGaa > 8:167553/1‑148 (MQ=255) aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTCCCCCAGGGTTGGTGTGATCTCGGGGGGGGCGGGCGGGCCCGCGCCGGGc > 7:158150/1‑148 (MQ=37) aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcg > 4:61181/1‑140 (MQ=255) gTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcgc < 3:61181/140‑1 (MQ=255) ggCCAAGGCCTCCCGGGCGGCGCGCGGGAGCGGCGTGCGCGGCGCCATGGCCCGGGGCGGCTTGGCCGCCCGGCGCGGGCACGCCCGCTCCCCCCGGGTGGCGGAGAGCTCTGGGGGAGCGGGCGGGCCCGCGAAGGGCCGCGAAGCg < 5:141311/148‑1 (MQ=255) ggCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTTCAGCCATTCGCGGGCACGCCCGCTCCCACAGGTGTGCTGCGCTCTCGGTGGGAGCGGGCGTGCCCGCGCCGGGCCGCGCAGCg > 5:130927/1‑148 (MQ=37) ctgcgcgcgGGCGCGGCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAAc < 3:136862/145‑1 (MQ=38) ttCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCGCACCCCCCGGGTTGCTGTGATATCATTGGGCGCGGGCCTGCCCCCGAAGCGCCGCGAAGCGGCCCCAAATGGTTccccc > 3:157587/1‑144 (MQ=255) aTCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCAACCACAGGTTTGCTGAGATAGATGTGGGAGCGGGCGTTCCCCCCAAGGGCCGCGAAGCGGCCCCAATTGCTTAAACCGTGGTTCTTTACGAGCACCGCGTg > 2:51307/1‑148 (MQ=37) aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCACCCACACGTTTGCTGAGATATCTGTGGGAGCGGGCGTGCCCGCCCAGGGCCGCGAACCGGCCCCAAATGCTTAAACCCTGGGTCTTGCCCAGCAGCGCGTGGTa > 6:61313/1‑147 (MQ=255) tgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATATCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCtt > 2:3357/1‑103 (MQ=255) gctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTCCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTc < 1:3357/103‑1 (MQ=255) cTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATcccc > 6:88881/1‑148 (MQ=255) | GGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCC > NC_002947/352930‑353208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |