| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 81.2% | 31.1 / 4.6 | 17 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (6/7); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 94.1% | 43.5 / ‑6.8 | 17 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (6/10); minor base C (1/0); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑‑TCG‑GG‑CA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCC > NC_002947/1499367‑1499638 || gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑cg‑cccgctc < 7:146148/148‑6 (MQ=25) aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACG‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 8:35790/148‑1 (MQ=21) agcagGACTGAGGCGGCCAAA‑GGGTGGGAGGGGGCGTGCCCGCGAAACAGGCGCGGCGGGGCTTGGCACC‑GGCTTTGCGGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACGGTGACGGc < 4:17057/148‑1 (MQ=21) gCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGCAAAAGGCGCCCCCGGGCATGGCACC‑CGCGTTTCCCGG‑GT‑TAGCCG‑GG‑AC‑CC‑CCGCGCCCACAGACAGAATTGCAATAAACGGGGCGATAGCAAAAGTGCCGGCCCGGTTTTTTTTTGc > 2:14940/1‑148 (MQ=255) ccAAACCGGGGGGCGCGGGGGGCCCCGCGAAACCGGCGCCGCGGGGCAGGCCCCG‑GGCTTGGCGGGTGGG‑C‑‑GCG‑GC‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGcc < 1:116648/148‑1 (MQ=255) gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAAc < 8:119033/148‑1 (MQ=21) tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTcc > 2:191712/1‑148 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCggg > 4:67727/1‑90 (MQ=14) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 3:67727/90‑1 (MQ=14) cgggCGCCGGGGTGC‑TGGGCCC‑GGGTTTGCCGGG‑GT‑T‑‑GCGCGGCCACCC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 2:90485/146‑1 (MQ=255) aGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GGTTTTTGTTGGCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 4:22774/1‑148 (MQ=37) ggggcatgCCACGGGCTTGGCCGGT‑GTGC‑‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa < 1:147121/141‑1 (MQ=255) aTGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 1:39435/1‑135 (MQ=37) tggcacc‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:39435/129‑1 (MQ=37) gcacc‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGTTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 7:47231/5‑132 (MQ=38) cacc‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGTTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 8:47231/129‑1 (MQ=38) acc‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGt > 4:14158/3‑148 (MQ=38) gtgttcgcG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTcc > 3:60536/8‑148 (MQ=255) gtgttcgcG‑GG‑AA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTcc < 1:47526/141‑1 (MQ=255) || GCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑‑TCG‑GG‑CA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCC > NC_002947/1499367‑1499638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |