| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 83.3% | 30.1 / ‑0.1 | 18 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base A (7/8); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 94.4% | 38.3 / ‑5.7 | 18 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (7/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑‑TCG‑GG‑CA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGA > NC_002947/1499341‑1499615 || aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCttt < 3:46737/148‑1 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTTCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑CGCTTTTCCGgt > 8:146148/1‑148 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgt < 7:134516/148‑1 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTTCCCGCGCAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCCgt‑g > 7:14520/1‑148 (MQ=39) gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCAAAGCCGgt‑gcca < 2:86104/148‑4 (MQ=38) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑cg‑cccgctc < 7:146148/148‑6 (MQ=25) aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACG‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 8:35790/148‑1 (MQ=21) agcagGACTGAGGCGGCCAAA‑GGGTGGGAGGGGGCGTGCCCGCGAAACAGGCGCGGCGGGGCTTGGCACC‑GGCTTTGCGGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACGGTGACGGc < 4:17057/148‑1 (MQ=21) gCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGCAAAAGGCGCCCCCGGGCATGGCACC‑CGCGTTTCCCGG‑GT‑TAGCCG‑GG‑AC‑CC‑CCGCGCCCACAGACAGAATTGCAATAAACGGGGCGATAGCAAAAGTGCCGGCCCGGTTTTTTTTTGc > 2:14940/1‑148 (MQ=255) ccAAACCGGGGGGCGCGGGGGGCCCCGCGAAACCGGCGCCGCGGGGCAGGCCCCG‑GGCTTGGCGGGTGGG‑C‑‑GCG‑GC‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGcc < 1:116648/148‑1 (MQ=255) gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAAc < 8:119033/148‑1 (MQ=21) tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTcc > 2:191712/1‑148 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCggg > 4:67727/1‑90 (MQ=14) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 3:67727/90‑1 (MQ=14) cgggCGCCGGGGTGC‑TGGGCCC‑GGGTTTGCCGGG‑GT‑T‑‑GCGCGGCCACCC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 2:90485/146‑1 (MQ=255) aGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GGTTTTTGTTGGCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 4:22774/1‑148 (MQ=37) ggggcatgCCACGGGCTTGGCCGGT‑GTGC‑‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGa < 1:147121/141‑1 (MQ=255) aTGGCACC‑GGCTTTGCCGGT‑GT‑TC‑GCG‑GG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 1:39435/1‑135 (MQ=37) || ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAA‑CGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑‑TCG‑GG‑CA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGA > NC_002947/1499341‑1499615 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |