Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,921,460 G→A G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA96.8% 95.0 / NA 31G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/10);  minor base C (0/1);  total (20/11)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAT  >  NC_002947/2921315‑2921603
                                                                                                                                                  |                                                                                                                                                
cacGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCaggg                                                                                                                                               <  6:234116/149‑1 (MQ=255)
          ggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCaa                                                                                                                                     <  8:64864/149‑1 (MQ=255)
                         gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                      >  1:26286/1‑149 (MQ=255)
                          aCAACCGGCCCGGGCGCGCTGGCGCTGACCGCCAGGATGGCGGCACCGGCAATGTCACCCCGGAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCCGGGGGGCCGGCAACGACTTCCTCAACGGt                                                                                                                     <  8:204993/149‑1 (MQ=255)
                               cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGCCACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAATGGTGCgg                                                                                                                 >  8:98405/1‑148 (MQ=255)
                                          cgcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATa                                                                                                     <  1:1770/149‑1 (MQ=255)
                                           gcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGTCACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCACTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATa                                                                                                     <  1:91231/149‑1 (MQ=255)
                                                 gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑Cggtggt                                                                                             >  4:176930/1‑149 (MQ=255)
                                                        gCCTGGACGGCGGCCACGGCAAGGACACCCGGCAGGGCGGCGATGGTCACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGtgggtggg                                                                                      <  4:352859/149‑1 (MQ=255)
                                                         ccTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTgggg                                                                                     >  2:104620/1‑149 (MQ=255)
                                                                 gcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTaa                                                                              <  2:26286/148‑1 (MQ=255)
                                                                          gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                     >  8:75823/1‑148 (MQ=255)
                                                                                    ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                           >  5:44021/1‑148 (MQ=255)
                                                                                    ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                           >  7:210701/1‑148 (MQ=255)
                                                                                                       aaCGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                         >  5:132480/1‑147 (MQ=255)
                                                                                                        aCGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa                                       <  6:44021/148‑1 (MQ=255)
                                                                                                             acaTTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                                 <  7:75823/149‑1 (MQ=255)
                                                                                                               aTTGTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                               >  6:20688/1‑149 (MQ=255)
                                                                                                                  gTTCGGCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                            >  6:141273/1‑149 (MQ=255)
                                                                                                                       gCGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt                       <  6:132480/149‑1 (MQ=255)
                                                                                                                        cGACGTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg                      <  5:141273/149‑1 (MQ=255)
                                                                                                                            gTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTACCGATA‑CGGTGGTGGGTGGGGCCGGTAACGCCACCATGATCGCCACGGATGGATGCGCTGTGTTCCAGTTCGCTGGAGGGTTTgggct                  >  6:238460/1‑146 (MQ=255)
                                                                                                                            gTGGGTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGCAACGATAGTAGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCCCCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGc                    >  1:300820/1‑148 (MQ=255)
                                                                                                                               ggTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGa               >  6:330611/1‑149 (MQ=255)
                                                                                                                                gTACCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGGAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCAAGTTCGCTGCAGGCTTTGGCAACGAc              >  8:72364/1‑149 (MQ=255)
                                                                                                                                  aCCGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCt            >  3:199175/1‑149 (MQ=255)
                                                                                                                                    cGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGCCACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGa          >  6:96560/1‑149 (MQ=255)
                                                                                                                                    cGACACC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCCACGACCTGa          >  4:281270/1‑149 (MQ=255)
                                                                                                                                       cacC‑CTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatca       <  5:20688/149‑1 (MQ=255)
                                                                                                                                          c‑cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc     >  5:180341/1‑148 (MQ=255)
                                                                                                                                             tGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAt  >  1:64042/1‑149 (MQ=255)
                                                                                                                                             tGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAt  >  1:58873/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACC‑CTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATA‑CGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAT  >  NC_002947/2921315‑2921603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑