Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	101.8 / NA	30	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/18);  total (12/18)

CATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGAT  >  NC_002947/499063‑499335                                                                                                                                              |                                                                                                                                       cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGcc                                                                                                                                >  4:188004/1‑149 (MQ=255)       cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACTGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGaa                                                                                                                          <  5:147819/149‑1 (MQ=255)          ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACgg                                                                                                                       <  3:141038/149‑1 (MQ=255)           cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACggg                                                                                                                      <  6:247478/149‑1 (MQ=255)               aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctg                                                                                                                  <  7:85146/149‑1 (MQ=255)                ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCATGCCC‑CGAACCggccgtg                                                                                                                 >  3:172391/1‑146 (MQ=255)                ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGGGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctgg                                                                                                                 <  7:12000/149‑1 (MQ=255)                  cACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctggcc                                                                                                               >  4:6755/1‑149 (MQ=255)                      ccTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCctgct                                                                                                           <  6:68828/149‑1 (MQ=255)                       cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCctgctg                                                                                                          >  3:308334/1‑149 (MQ=255)                       cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCctgct                                                                                                           >  3:303134/1‑148 (MQ=255)                         gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGa                                                                                                         >  1:245945/1‑148 (MQ=255)                          aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGAt                                                                                                        <  3:6755/148‑1 (MQ=255)                                            aaCTCGCTGGCGGAGCTGGTGAAGTACTCCTTGGAGGACAAGCTGTTCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGG‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCgtc                                                                                      <  4:343174/148‑3 (MQ=255)                                            aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑c                                                                                     >  2:70145/1‑148 (MQ=255)                                                              gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGcc                                                                   <  3:188004/147‑1 (MQ=255)                                                              gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGCCCg                                                                 >  2:44727/1‑149 (MQ=255)                                                                              ctcaaaaGCTTATCTTATTGCCACATACGGGGGCCCGAAACTTGCCTACGCCTTTGTGGGCGG‑GGGCAGGCCCGCTAACGGGCCTGGCCTGCTTATCTCTATGGCTGACCCGG‑CCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGt                                                 <  4:308334/144‑1 (MQ=25)                                                                              accacaCGCTTTTCTGATTCCTACATACGGGCGCCCTAAACTTGCCCACGCATTTTTGGGCGG‑GGGATTGCCCGCTAAGGGGCCTGGCGGGCTTATCTCTTTGGCTGAGCCGG‑CCTCT‑GCGCGGGAATGCCCGCTCCCACAGGTTTCGt                                                 <  4:303134/147‑1 (MQ=255)                                                                                  cacgcggatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGtt                                             <  1:56755/143‑1 (MQ=255)                                                                                          tctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCGGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCCTGCCCGCTCCCCCAGGTTTCGTGGTTGGcagctc                                     >  5:259532/1‑144 (MQ=255)                                                                                           ctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCTCTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGc                                      <  1:70145/148‑1 (MQ=255)                                                                                                   gACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTc                            <  3:241954/149‑1 (MQ=255)                                                                                                                ccGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATTCCCGCGAACGGGCCTGGCCAGCTGATCTCTATGGCTGAGCCGG‑CCGCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTGGTGGTTTGCTAGCAAGGGCATTc                            >  2:287173/1‑136 (MQ=38)                                                                                                                ccGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTc                            <  1:287173/137‑1 (MQ=255)                                                                                                                     aCTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGCCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGt          <  8:113167/149‑1 (MQ=255)                                                                                                                         gCCTAAGCCTATGTGGGAGC‑GGGCTGGCCCGCTAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg       <  2:245945/148‑1 (MQ=39)                                                                                                                             aaGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATGTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGGATGCCCGCTCCCCCAGGGTTCGTGGTTGGCTCGCAAGGGGATTCCAATCCCGCCCTCCTTGTTGGGCGAt  >  7:232766/1‑149 (MQ=255)                                                                                                                                    tgtgGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg                                                                                                <  6:222082/50‑1 (MQ=255)                                                                                                                                    tgtgGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg                                                                                                >  5:222082/1‑50 (MQ=255)                                                                                                                                              |                                                                                                                                       CATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGG‑CCTCT‑TCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGAT  >  NC_002947/499063‑499335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑