| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,740,819 | 2 bp→CT | intergenic (+56/+696) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,819 | 0 | T | C | 97.6% | 112.3 / NA | 42 | intergenic (+56/+697) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (21/20); minor base A (1/0); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 4,740,820 | 0 | G | T | 100.0% | 138.7 / NA | 42 | intergenic (+57/+696) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/20); total (22/20) | |||||||||||
CCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATC > NC_002947/4740678‑4740956 || ccAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg < 7:201840/149‑1 (MQ=255) cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc > 1:16560/1‑149 (MQ=255) aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg > 5:163525/1‑149 (MQ=255) aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGGCGAACGCAAGGGAGAGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc > 1:120370/1‑149 (MQ=255) aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGc < 6:163525/149‑1 (MQ=255) tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt > 2:91422/1‑149 (MQ=255) gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGGTTGCCGTGAAGGACCGCAAGTAGGATGAGGAAGCCGACCGCAA‑‑GA‑AGC‑ATGCCGACGGGCGGCGTTTTTTT‑TTTTTCATTTGCCGGCAAGTACCCGGCCTTGc > 1:76953/1‑149 (MQ=255) cgcCAGCTGTACACCGGCGAGCAGAAGCCCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TGTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa > 1:207257/1‑147 (MQ=255) gcCAGCTGTACTCCGGCGAGGAGAAGCGCGACATC‑GTTGCGCTGAAAGACGGCAAGTAAGCTGCAGTTGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCTGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCgaggag < 4:163490/149‑1 (MQ=255) cTGAACACCGGCGAGCACAAGCGCGACATC‑CTTGCCCTGAAAGACCGCAAGAAAGCTGCAGTAGCCGAACGCAA‑‑CA‑CCG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCgg < 8:239679/149‑1 (MQ=255) cGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCCGCCGTGGCCCGGCAGCGGGCGGCCCAggcc > 7:120825/1‑146 (MQ=255) gCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCAGCACAGacac > 5:150535/1‑148 (MQ=255) gAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTGGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAcacca > 1:321996/1‑148 (MQ=255) cgcgACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCa < 2:76953/149‑1 (MQ=255) aCATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGGACTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGt‑t > 1:97497/1‑149 (MQ=255) ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGt‑ttttatac > 2:116590/1‑145 (MQ=255) cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCt < 7:95542/134‑1 (MQ=255) cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCt > 8:95542/1‑134 (MQ=39) cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTccgcc > 8:236603/1‑149 (MQ=255) ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTccgccc > 7:161032/1‑149 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTccgcccg > 5:100705/1‑149 (MQ=255) tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCCGCCGTGCCCGCGAAGAGGCCGGCACCGCCCCCACAAAACCTCAGT‑GTTTAGccgccc > 7:240116/1‑147 (MQ=255) aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGACTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCAc > 6:120650/1‑149 (MQ=255) gTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACAACAAAACCTAAGG‑TTTTCTCCGACCGCTCACTCAGCCAtt > 2:244405/1‑149 (MQ=255) tAAGCTGAAGTAGCCTAACGCAA‑‑CA‑AGG‑CTGCCCTGGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCttt < 3:55338/149‑1 (MQ=255) aGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTc < 2:16560/148‑1 (MQ=255) gCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAg < 3:235578/149‑1 (MQ=255) gCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTACGCCCGCTCACGCAGCCCTTTCAg > 1:78891/1‑149 (MQ=255) tGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTACGCCCGCTCACGCAGCCCTTTCAggg > 5:204501/1‑149 (MQ=255) ccGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTATCCGCCCGCTCCCGCACCCCATTCAGGGTATTGAAc > 8:206982/1‑149 (MQ=255) aaCGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg > 4:283559/1‑137 (MQ=39) aaCGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg < 3:283559/137‑1 (MQ=255) agG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTCCTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac < 1:91422/149‑1 (MQ=255) agG‑CTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac < 8:161032/148‑1 (MQ=255) gg‑CTGCCCTCAGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTGTTCGCGCCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca < 1:190283/149‑1 (MQ=255) cccTCGGGCTGCC‑TTTTTTATTTTTCCTGGGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc < 1:244405/149‑1 (MQ=255) cTCGGGCAG‑CATTTTTTATTTTTCCTGTGCCGGCCTCTTCGCGGCGTTGCCCGCGAAGTGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt < 2:78891/149‑1 (MQ=255) gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt < 6:100705/149‑1 (MQ=255) ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCTCAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg < 1:116590/149‑1 (MQ=255) agccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcc < 7:236603/145‑1 (MQ=255) gccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCAGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcca < 6:150535/146‑1 (MQ=255) tttATTTTTCCTGTGCCGGCCTCTGCTCGGCCTTGCCCGCGAAGAGGCCGTCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGTCGCATCCACCACGAACTTGTTGGCCACCATc < 7:28433/148‑1 (MQ=255) || CCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATC‑GTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAA‑‑GA‑AGG‑CTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGT‑TTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATC > NC_002947/4740678‑4740956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |