Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 503,825 Δ1 bp 9.1% intergenic (+28/‑123) PP_0414 → / → rpe polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947503,8250G. 9.1% 133.8 / 7.2 44intergenic (+28/‑123)PP_0414/rpepolyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base . (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTC  >  NC_002947/503678‑503973
                                                                                                                                                   |                                                                                                                                                     
cTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTcgc                                                                                                                                                      >  4:198528/1‑149 (MQ=255)
 tGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTcgcg                                                                                                                                                     >  7:214006/1‑149 (MQ=255)
        gACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAg                                                                                                                                              >  6:247427/1‑149 (MQ=255)
               gCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑Gaa                                                                                                                                       <  8:214006/148‑1 (MQ=255)
               gCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑Gaa                                                                                                                                       <  3:198528/148‑1 (MQ=255)
               gCCGCGGCGGCGACATTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑Gaa                                                                                                                                       <  3:42525/148‑1 (MQ=255)
                 cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAagag                                                                                                                                    <  5:315330/149‑1 (MQ=255)
                   cggcggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTACCTGTGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGc                                                                                                                                  <  2:123689/149‑1 (MQ=38)
                     gcggcgTCCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGGTGACCCTGGAGTGGTTGCGTGGGGGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCg                                                                                                                                <  8:258376/149‑1 (MQ=255)
                     gcggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCg                                                                                                                                <  4:4020/149‑1 (MQ=255)
                      cggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCgg                                                                                                                               <  2:47273/149‑1 (MQ=255)
                         cgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGca                                                                                                                             >  1:62224/1‑148 (MQ=255)
                          gACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGca                                                                                                                             >  4:148475/1‑147 (MQ=255)
                                    aTTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTg                                                                                                                 <  7:59804/149‑1 (MQ=255)
                                                     gttgCTGCTGACCCTGGAGTGGTTGCCTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTAGATGATGGCTTGCCTTTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCTGAACAGGATGCGTTGGTCGACACCAATTGAt                                                                                                 >  3:198475/1‑148 (MQ=255)
                                                      ttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc                                                                                               <  2:62224/149‑1 (MQ=255)
                                                              gACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGc                                                                                       >  6:146461/1‑149 (MQ=255)
                                                               aCCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGtt                                                                                                                  >  7:139764/1‑121 (MQ=255)
                                                               aCCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGtt                                                                                                                  <  8:139764/121‑1 (MQ=255)
                                                                 ccTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCt                                                                                     >  3:122308/1‑148 (MQ=255)
                                                                     gAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCa                                                                                  <  4:122308/147‑1 (MQ=255)
                                                                          gTTGCTTGGTCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATc                                                                                <  4:181980/144‑1 (MQ=255)
                                                                          gTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAAACCAATCAGCCTTCATCgatat                                                                           >  3:181980/1‑145 (MQ=255)
                                                                           ttGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt                                                                                                 <  6:329515/126‑1 (MQ=255)
                                                                           ttGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt                                                                                                 >  5:329515/1‑126 (MQ=255)
                                                                                         cGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGTGTTGCCTGTACGGGCCTCTTCGCGGCTAAAGCGG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAAt                                                             <  4:198475/148‑1 (MQ=255)
                                                                                            gaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                         <  3:235087/149‑1 (MQ=255)
                                                                                                  gAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCATTCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCAcc                                                   >  5:278024/1‑149 (MQ=255)
                                                                                                      aCTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGa                                               <  5:145458/149‑1 (MQ=255)
                                                                                                          aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGATGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAt                                             <  5:247427/147‑1 (MQ=255)
                                                                                                          aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                                           >  2:41329/1‑149 (MQ=255)
                                                                                                               ccGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCaa                                      >  4:147656/1‑149 (MQ=255)
                                                                                                                 ggCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa                                    <  1:41329/149‑1 (MQ=255)
                                                                                                                            ggCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTGCAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATg                         >  6:161798/1‑149 (MQ=255)
                                                                                                                               caaccTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGTACAGGTTGCGTTGGTCGACAACAATTGATACCAATCAGCCTTAATCGCTTTCCCTGAGTTACAAAGCGCCCCACCGTGAATTTGCCAACAGGTGCGCCATGCAg                      >  6:135739/4‑149 (MQ=255)
                                                                                                                                  ccTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGccc                   >  6:253509/1‑149 (MQ=255)
                                                                                                                                        ccGGCGTCTTC‑AGGCTTTAGCCGCGATGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTCAGTTACAATGCGCTCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGc              <  7:288503/148‑1 (MQ=255)
                                                                                                                                        acGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGCACACGTTGCGATGGTCCACACCAATTGAAACCAATCAGCCAACATAGCTATCCCTGAGTTACAATGCGCGCCACCGACAATATGCCAACAGGAGCGCCATGCAGCCCTAccc              >  8:288503/2‑146 (MQ=25)
                                                                                                                                         cGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                         <  5:133266/104‑1 (MQ=255)
                                                                                                                                         cGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                         >  6:133266/1‑104 (MQ=255)
                                                                                                                                         cGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTa            >  7:162596/1‑149 (MQ=255)
                                                                                                                                         cGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTa            >  1:54248/1‑149 (MQ=255)
                                                                                                                                           gCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt          <  3:148475/149‑1 (MQ=255)
                                                                                                                                             ctctTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACAACAATTGATACCAATCAGCCTTCATCTCTTTCCCTGAGTTACAATGCGAGCCACCCTGAATTTGCCAAAAGGTGCGCCATGCAGCCCTACGCTATTGc        >  4:222198/1‑149 (MQ=37)
                                                                                                                                                ttCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcccc     >  7:126804/1‑149 (MQ=255)
                                                                                                                                                ttCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcccc     >  4:152087/1‑149 (MQ=255)
                                                                                                                                                   gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTc  >  8:160165/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTC  >  NC_002947/503678‑503973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑