Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 557,332 Δ1 bp 10.9% intergenic (+75/‑134) rpsN → / → rpsH 30S ribosomal protein S14/30S ribosomal protein S8

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947557,3320T.10.9% 134.2 / 10.7 46intergenic (+75/‑134)rpsN/rpsH30S ribosomal protein S14/30S ribosomal protein S8
Reads supporting (aligned to +/- strand):  ref base T (19/22);  new base . (2/3);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGGCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAG  >  NC_002947/557185‑557477
                                                                                                                                                   |                                                                                                                                                  
ccTGGGCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCta                                                                                                                                                   >  1:7327/1‑149 (MQ=255)
  tGGGCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCtata                                                                                                                                                 >  4:30275/1‑149 (MQ=255)
    ggCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑g                                                                                                                                              <  3:117596/149‑1 (MQ=255)
     gCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑gg                                                                                                                                             <  5:277601/149‑1 (MQ=255)
            cATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCTGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTg                                                                                                                                       >  1:188296/1‑148 (MQ=255)
            cATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCTGGTCTGGTCAAGGCATCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTg                                                                                                                                       <  2:188296/148‑1 (MQ=255)
            cATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgt                                                                                                                                      <  6:248616/149‑1 (MQ=255)
             aTGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtt                                                                                                                                     >  6:50469/1‑149 (MQ=255)
              tgctgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgttt                                                                                                                                    <  7:311/149‑1 (MQ=255)
                ctgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGCCTTgtttcg                                                                                                                                  >  4:184417/1‑149 (MQ=255)
                ctgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgtttg                                                                                                                                   <  2:315867/148‑2 (MQ=255)
                 tgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgttt                                                                                                                                    >  4:213336/1‑146 (MQ=255)
                 tgcGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCC‑ATATAGGGGCTTgttt                                                                                                                                    <  3:213336/146‑1 (MQ=255)
                       aaGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGc                                                                                                                           >  3:31361/1‑149 (MQ=255)
                        aGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGcc                                                                                                                          <  6:118662/149‑1 (MQ=255)
                        aGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGcc                                                                                                                          >  5:265788/1‑149 (MQ=255)
                                                  gTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGTGTGGAAGGGGAGCAATCTTCCGTCCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCATGTTTCGATTCTGCCTTGTGACTAGAAAGACCGCCTCAcat                                                                                                >  5:225668/1‑147 (MQ=255)
                                                       gTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgttt                                                                                                                               >  2:8410/1‑113 (MQ=255)
                                                       gTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTgtttcgttt                                                                                                                               <  1:8410/113‑1 (MQ=255)
                                                        tCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCt                                                                                          >  1:319049/1‑149 (MQ=255)
                                                           aGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGTAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTggg                                                                                       <  4:31361/149‑1 (MQ=255)
                                                                               cttgCAAGGCGCGGGTGGAAGGGGAGGAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCGTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCgg                                                                   <  6:225668/146‑1 (MQ=255)
                                                                                     aGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGtt                                                             <  2:319049/149‑1 (MQ=255)
                                                                                      ggCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTg                                                            <  4:90043/149‑1 (MQ=255)
                                                                                      ggCGCGGGTGCAAGGGGAGCAATCTTCCGACCGCCGGTGAACTTGCCAACAAACAAGCCGCTATAT‑GGGGCTTGTTTCGTTTCAGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTg                                                            <  3:184417/149‑1 (MQ=255)
                                                                                       gcgcGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGt                                                           <  2:7327/149‑1 (MQ=255)
                                                                                         gcgGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCggg                                                                  >  1:278313/1‑140 (MQ=255)
                                                                                         gcgGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCggg                                                                  <  2:278313/140‑1 (MQ=255)
                                                                                                  aGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGc                                                  >  7:244235/1‑147 (MQ=255)
                                                                                                         cAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCtaat                                         <  5:50469/149‑1 (MQ=255)
                                                                                                              ttCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGTGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATa                                                       <  1:34748/130‑1 (MQ=255)
                                                                                                              ttCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGAGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATa                                                       >  2:34748/1‑130 (MQ=255)
                                                                                                                ccGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCt                                   <  6:265788/148‑1 (MQ=255)
                                                                                                                 cGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCtt                                  <  3:30275/148‑1 (MQ=255)
                                                                                                                     cgccgGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGttttt                                                                                  <  1:90563/96‑1 (MQ=255)
                                                                                                                     cgccgGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGttttt                                                                                  >  2:90563/1‑96 (MQ=255)
                                                                                                                                ttGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATc                    >  5:109344/1‑147 (MQ=255)
                                                                                                                                ttGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATc                    >  3:256574/1‑147 (MQ=255)
                                                                                                                                ttGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATc                    >  5:330962/1‑147 (MQ=255)
                                                                                                                                        aaacaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGa          <  6:109344/149‑1 (MQ=255)
                                                                                                                                          acaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCtt                                  <  1:274192/123‑1 (MQ=255)
                                                                                                                                          acaaGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCtt                                  >  2:274192/1‑123 (MQ=255)
                                                                                                                                            aaGCCCC‑ATATAGGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTAt      >  6:5217/1‑149 (MQ=255)
                                                                                                                                             aGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATg     >  2:136498/1‑149 (MQ=255)
                                                                                                                                              gCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATg     >  3:45197/1‑148 (MQ=255)
                                                                                                                                                cccTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCAt            <  3:161372/139‑1 (MQ=255)
                                                                                                                                                cccTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCAt            >  4:161372/1‑139 (MQ=255)
                                                                                                                                                cccTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAg  <  4:256574/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTGGGCCGTAACATGCTGCGTCAAGCTGCAATGCGCGGTGACGTACCAGGTCTGGTCAAGGCCTCCTGGTAATCGCTGCAGGCAAGGCGCGGGTGGAAGGGGAGCAATCTTCCGACCGCCGGTGACCTTGCCAACAAACAAGCCCCTATAT‑GGGGCTTGTTTCGTTTCTGCCTTGTGTCTAGAATGACCGGCTCACCTGAGCCTGGGTTTTTGCCCAAACGGTTCGGGTGGTTGTCATAGCCGCAAGGCTAATAATTCTTGTATCAGGAGCATCTAGCCCATGAGTATGCAG  >  NC_002947/557185‑557477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑