| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 587,042 | Δ1 bp | 8.9% | coding (94/810 nt) | PP_0497 → | Cro/CI family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 587,042 | 0 | C | . | 8.9% | 139.5 / 7.1 | 45 | coding (94/810 nt) | PP_0497 | Cro/CI family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (21/20); new base . (2/2); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01 | |||||||||||
ACTTGATGCTACAATGGCGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCC > NC_002947/586904‑587185 | acttgatgcTACAATGGCGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTcaacaa < 1:10041/149‑1 (MQ=255) ttgatgcTACAATGGCGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAg > 4:289013/1‑148 (MQ=255) tgcTACAATGGCGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCt < 2:293608/149‑1 (MQ=255) tACAATGGCGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAg > 4:99396/1‑149 (MQ=255) gCGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCa > 7:33372/1‑148 (MQ=255) cGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCaaa < 5:137405/149‑1 (MQ=255) ttagcgTCACCTGGATTCCGCATATGAGCGATGACTTCGCTACAATCCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGt < 2:212438/146‑1 (MQ=255) aCCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACgg < 3:289013/148‑1 (MQ=255) ccTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCt < 6:300888/149‑1 (MQ=255) tGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACg < 1:75386/144‑1 (MQ=255) tGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACg > 2:75386/1‑144 (MQ=255) ggATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCttt > 3:74270/1‑148 (MQ=255) gATTCCGCATAAGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCGGACGGCtttgc < 5:240886/149‑3 (MQ=255) ccGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCt > 5:114783/1‑149 (MQ=255) cGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGAc < 2:145061/136‑1 (MQ=255) cGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGAc > 1:145061/1‑136 (MQ=255) atatGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAg > 3:28187/1‑149 (MQ=255) gATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCgg < 4:28187/149‑1 (MQ=255) tCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGc > 4:212737/1‑148 (MQ=255) ctcgtCGCTACAAACCTCCACCGTGCCTGCAGTCTCTACCGCTCTTATTCCGCTGGTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGCCGGCTTTCAACCTGAAGCGTATCGGcta < 4:43976/145‑3 (MQ=255) cTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACttcttc < 8:33372/149‑1 (MQ=255) cAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCg < 3:99396/147‑1 (MQ=255) cTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTc > 1:38091/1‑147 (MQ=255) cTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTACAACCTGAAGCGTATCGGCGACTTCTTCGGTGTc > 3:43872/1‑147 (MQ=255) tCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAg > 6:273270/1‑149 (MQ=255) aCTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGAt > 2:306808/1‑148 (MQ=255) gCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACg < 4:74270/148‑1 (MQ=255) cTGCAGTCACTACCGCGCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCCTCGGTGTCGAGGATTACGAAc < 8:63055/149‑1 (MQ=255) gTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGa > 1:12051/1‑90 (MQ=255) gTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGa < 2:12051/90‑1 (MQ=255) tCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACg < 2:50019/140‑1 (MQ=255) tCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACg > 1:50019/1‑140 (MQ=255) aCTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTg > 3:68245/1‑149 (MQ=255) ccGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGccc < 1:306808/148‑1 (MQ=255) ctctATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCG‑AGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCccgcc > 3:64544/1‑147 (MQ=255) ctctATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCG‑AGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCccgcc > 5:129902/1‑147 (MQ=255) ccGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTc > 8:109437/1‑149 (MQ=255) cGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCg > 2:15057/1‑149 (MQ=255) tGCCGCCAGCTGTCGATCAACCGCGCG‑AGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCt < 6:129902/148‑1 (MQ=255) tGCCGCCAGCTGTCGATCAACCGCGCG‑AGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCt < 4:64544/148‑1 (MQ=255) tGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTgcg > 1:287148/1‑149 (MQ=255) tCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGcgcg > 7:160151/1‑149 (MQ=255) tCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGcgcg < 2:38091/149‑1 (MQ=255) cGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCgg < 7:109437/149‑1 (MQ=255) gATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCggg > 4:250113/1‑149 (MQ=255) gcgcgcAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGccc < 5:273270/149‑1 (MQ=255) | ACTTGATGCTACAATGGCGTCATTCGCGTCACCTGGATTCCGCATATGAGCGATCACTTCGCTACAAACCTCAAACTTGCCTGCAGTCACTACCGCTCTATTTCCGAAGTTTGCCGCCAGCTGTCGATCAACCGCGCGCAGTTCAACAAGTACCTGAGCGGGCAAAGCCGTCCGACGGCTTTCAACCTGAAGCGTATCGGCGACTTCTTCGGTGTCGAGGATTACGAACTGAACCTGCCCGCCGAGCAGTTCGCCCGTCTGATCGGTGCGCGGGTATCGCCC > NC_002947/586904‑587185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |