Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,068,520	Δ1 bp	9.4%	coding (686/948 nt)	PP_0926 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,068,520	0	C	.	9.4%	199.6 / 13.7	64	coding (686/948 nt)	PP_0926	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (28/30);  new base . (2/4);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTACGTGATGG  >  NC_002947/1068373‑1068663                                                                                                                                                    |                                                                                                                                                 ccGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGcc                                                                                                                                                 <  3:9350/149‑1 (MQ=255)     gcCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCg                                                                                                                                             <  4:178116/149‑1 (MQ=255)       ccATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAt                                                                                                                                           <  1:198168/149‑1 (MQ=255)        cATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGG‑CATCGAT‑gg                                                                                                                                        <  1:98993/149‑2 (MQ=255)             cTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGc                                                                                                                                    >  2:94809/1‑149 (MQ=255)             cTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGc                                                                                                                                    >  5:40851/1‑149 (MQ=255)              ttACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCt                                                                                                                                   >  8:171916/1‑149 (MQ=255)                aCGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGG‑CATCGATGGCCAGccg                                                                                                                                  <  8:44239/148‑3 (MQ=255)                 cGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGG‑CATCGATGGCCAGccga                                                                                                                                 <  6:19656/148‑4 (MQ=255)                  ggCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGt                                                                                                                               <  1:82581/149‑1 (MQ=255)                  ggCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGACCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGt                                                                                                                               <  4:137492/149‑1 (MQ=255)                  ggCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGACCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGt                                                                                                                               >  3:137492/1‑149 (MQ=255)                    ccAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGa                                                                                                                             <  7:171916/149‑1 (MQ=255)                    ccAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGG‑CATCGATGGCCAGc                                                                                                                                    <  4:50303/142‑1 (MQ=255)                    ccAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGG‑CATCGATGGCCAGc                                                                                                                                    >  3:50303/1‑142 (MQ=255)                       ggTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGaaaa                                                                                                                          <  1:291221/149‑1 (MQ=255)                           ccGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCCGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGct                                                                                                                      <  6:9431/149‑1 (MQ=255)                             gCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGctct                                                                                                                    <  6:40851/149‑1 (MQ=255)                                 cccAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTgg                                                                                                                  <  2:198054/147‑1 (MQ=255)                                   cAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGc                                                                                                              <  6:31791/149‑1 (MQ=255)                                       tGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGAAACTGTGGTCGGCTGGCCATCGAT‑GCAAGCTTCGTGAAAATGCTCTGGCTGCCgct                                                                                                          >  8:12161/1‑149 (MQ=255)                                                aCCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGc                                                                                                   <  4:272106/147‑1 (MQ=255)                                                              ccTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGt                                                                                   >  7:104625/1‑149 (MQ=255)                                                              ccTGGCACAGATGACCCTGCCACTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGt                                                                                   >  1:287438/1‑149 (MQ=255)                                                               cTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGtt                                                                                  >  1:78380/1‑149 (MQ=255)                                                               cTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGtt                                                                                  >  1:247530/1‑149 (MQ=255)                                                                tGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTtg                                                                                 >  5:296641/1‑149 (MQ=255)                                                                    accgATGACCCTGCGGCTGGCGGTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCg                                                                             <  2:247530/146‑1 (MQ=255)                                                                    acaGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCg                                                                             >  5:291126/1‑149 (MQ=255)                                                                    acaGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCg                                                                             >  5:291128/1‑149 (MQ=255)                                                                            cccTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTcgc                                                                     <  8:104625/149‑1 (MQ=255)                                                                            cccTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCGGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTcgc                                                                     >  2:91056/1‑149 (MQ=255)                                                                              cTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCgg                                                                   >  1:21358/1‑149 (MQ=255)                                                                              cTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCgg                                                                   <  1:94809/149‑1 (MQ=255)                                                                              cTGCCACTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCgg                                                                   <  2:287438/149‑1 (MQ=255)                                                                               tGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCgg                                                                            >  3:31004/1‑139 (MQ=255)                                                                               tGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCgg                                                                            <  4:31004/139‑1 (MQ=255)                                                                               tGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCggg                                                                  >  7:244082/1‑149 (MQ=255)                                                                                  cGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCg                                                               >  5:280624/1‑149 (MQ=255)                                                                                     tGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAg                                                            <  6:291128/149‑1 (MQ=255)                                                                                       gcgcTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCTGAg                                                            <  6:291126/147‑1 (MQ=255)                                                                                         gcTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTgg                                                        <  3:238415/149‑1 (MQ=255)                                                                                                  tATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATc                                                   <  2:57618/145‑1 (MQ=255)                                                                                                  tATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGAGGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATc                                                   <  7:12161/145‑1 (MQ=255)                                                                                                  tATCGGCGGCACGCTGTCGCTGGCAGCGCCGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCtt                                                <  1:91056/148‑1 (MQ=255)                                                                                                     cggcggCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTc                                             <  2:21358/148‑1 (MQ=255)                                                                                                                 gTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTc                                             >  7:83482/1‑136 (MQ=255)                                                                                                                 gTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTc                                             <  8:83482/136‑1 (MQ=255)                                                                                                                  tCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCa                                <  6:280624/148‑1 (MQ=255)                                                                                                                     cTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCgg                                                                            <  1:47972/101‑1 (MQ=255)                                                                                                                     cTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCgg                                                                            >  2:47972/1‑101 (MQ=255)                                                                                                                             gcTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCt                                           <  6:268971/126‑1 (MQ=255)                                                                                                                             gcTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GACAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCt                                           >  5:268971/1‑126 (MQ=255)                                                                                                                                 cGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCg                  <  2:78380/147‑1 (MQ=255)                                                                                                                                   tAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCa              <  8:244082/149‑1 (MQ=255)                                                                                                                                    aaCTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCGTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCGTGTCCAGATGACGCCCGACCGCTGCGGcctg             >  8:223670/1‑147 (MQ=255)                                                                                                                                     aCTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGc            >  4:190208/1‑149 (MQ=255)                                                                                                                                       tgtgGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTa          >  7:27496/1‑149 (MQ=255)                                                                                                                                          acgaagCTGG‑CATCGATGGCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTACGt       >  5:213935/6‑149 (MQ=255)                                                                                                                                              ggCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTcc                                            >  8:234080/1‑108 (MQ=255)                                                                                                                                              ggCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTcc                                            <  7:234080/108‑1 (MQ=255)                                                                                                                                              ggCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTACGTGATg   >  7:276851/1‑149 (MQ=255)                                                                                                                                              ggCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTACGTGATg   >  5:44078/1‑149 (MQ=255)                                                                                                                                              ggCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCAGCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTACGTGATg   >  7:48641/1‑149 (MQ=255)                                                                                                                                               gCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTACGTGATgg  >  7:155907/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CCGCGCCCATGGCTTACGGCCAGGTGCCGCTGCCCAACTGGCTACTGACCTCGGGCGACTACCTGGCACAGATGACCCTGCCGCTGGCGCTGATCTGTATCGGCGGCACGCTGTCGCTGGCAGCGCTGCGTAACTGTGGTCGGCTGGCCATCGAT‑GCCAGCTTCGTGAAAATGCTCTGGCTGCCGCTGCTCGGCACCCTTGGGGCCTGGTTGTGCGGGTTTCGCGGGGCGGAGCTGGGCATCCTTTTCCTGTACATAGGCAGCCCGACCGCTGCGGCCAGCTACGTGATGG  >  NC_002947/1068373‑1068663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑