Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,417,328	Δ1 bp	5.4%	coding (576/759 nt)	PP_1239 →	metallo‑beta‑lactamase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,417,328	0	T	.	5.4%	222.1 / 6.5	74	coding (576/759 nt)	PP_1239	metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (34/34);  new base . (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

CGCTGTTGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGG  >  NC_002947/1417183‑1417470                                                                                                                                                  |                                                                                                                                                  cgcTGTTGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTgcg                                                                                                                                                >  7:224592/1‑149 (MQ=255)  gcTGTTGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGACTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTgcgc                                                                                                                                               >  2:175504/1‑149 (MQ=255)         cTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCATGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTgg                                                                                                                                        <  2:989/148‑1 (MQ=255)         cTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTgg                                                                                                                                        <  7:45293/148‑1 (MQ=255)         cTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTgg                                                                                                                                        <  2:309280/148‑1 (MQ=255)          tGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTg                                                                                                                                      >  5:166402/1‑149 (MQ=255)            gAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCT‑GCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑Ac                                                                                                                                 >  3:131234/1‑149 (MQ=255)             agtgTTACCAGGGCCTGGATGCAGTGCTGATCGAGGCTAACCACTTTCGCGACGTGGTGGCACGTGGTCACTACCCGGCTTTTCTGAATCAGCGTGTGGGTGGCATGCAAGGTGATTTGAACATCCTCGAGGGTGCGC‑GCCTGGTT‑C‑Ac                                                                                                                                 <  4:118380/146‑1 (MQ=255)                  tACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGtt                                                                                                                            >  8:35774/1‑149 (MQ=255)                   aCCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGtt                                                                                                                            <  8:224592/148‑1 (MQ=255)                   aCCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTg                                                                                                                           >  6:211042/1‑149 (MQ=255)                    ccAGGGCCTGGATGCACTGCTTATAGAGGCTAACCAATGTCGCGACCTGCTGGCACGTGGTCAGTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGtttg                                                                                                                          <  8:291942/149‑3 (MQ=255)                    ccAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTgg                                                                                                                          >  8:123326/1‑149 (MQ=255)                    ccAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCT‑GCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTgg                                                                                                                          <  4:131234/148‑1 (MQ=255)                    ccAGGGCCTGGATGCACTGCTGATCGAGGCTAAACACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTgg                                                                                                                          >  6:314770/1‑149 (MQ=255)                      aGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGc                                                                                                                        <  7:123326/149‑1 (MQ=255)                        ggCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTg                                                                                                                      >  2:37688/1‑149 (MQ=255)                         gCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTg                                                                                                                      >  8:96286/1‑148 (MQ=255)                                   aCTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGc                                                                                                           <  3:20142/149‑1 (MQ=255)                                       ctgATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGTCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCaa                                                                                                         <  1:175504/147‑1 (MQ=255)                                       ctgATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAaca                                                                                                       >  4:248065/1‑149 (MQ=255)                                        tgATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGCCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAacac                                                                                                      >  4:102862/1‑149 (MQ=255)                                         gATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACAcc                                                                                                     >  7:229510/1‑149 (MQ=255)                                          aTCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCt                                                                                                    >  7:11838/1‑149 (MQ=255)                                                  tAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTgg                                                                                            >  4:60016/1‑149 (MQ=255)                                                     ccACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGcc                                                                                          >  4:190754/1‑148 (MQ=255)                                                        cTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGAAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTACTGTCGAGAAGTTGGGGTGGAAAAACCTCAAACACATGTTGCTGGccac                                                                                        >  1:107501/1‑147 (MQ=255)                                                         tGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCt                                                                                     >  7:43100/1‑149 (MQ=255)                                                             gcgACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTcag                                                                                  <  3:102862/148‑1 (MQ=255)                                                                 ccTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCagcagc                                                                              >  1:252524/1‑148 (MQ=255)                                                                     ctgGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAg                                                                           <  5:314770/147‑1 (MQ=255)                                                                     ctgGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCCGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaa                                                                         >  3:231236/1‑149 (MQ=255)                                                                       ggCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaac                                                                        >  3:118384/1‑148 (MQ=255)                                                                             tGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGc                                                                 <  8:229510/149‑1 (MQ=255)                                                                             tGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCCGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGc                                                                 <  8:95441/149‑1 (MQ=255)                                                                               gTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGcc                                                                <  5:41922/148‑1 (MQ=255)                                                                                tCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCca                                                               >  8:168216/1‑148 (MQ=255)                                                                                      cccGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCt                                                                                              <  5:1899/111‑1 (MQ=255)                                                                                      cccGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCt                                                                                              >  6:1899/1‑111 (MQ=255)                                                                                      cccGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTgg                                                        <  3:190754/149‑1 (MQ=255)                                                                                       ccGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGATCAACCTGCCACACTTGGc                                                       >  2:221024/1‑149 (MQ=255)                                                                                         ggCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGccc                                                     <  5:211042/149‑1 (MQ=255)                                                                                                cTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCCGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGt                                               <  4:231236/148‑1 (MQ=255)                                                                                                  gAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACACCCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCt                                            >  1:215356/1‑149 (MQ=255)                                                                                                   aaGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCt                                            <  3:282088/148‑1 (MQ=255)                                                                                                    agcagcGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCttt                                          <  2:252524/149‑1 (MQ=255)                                                                                                     gcagcGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTg                                         >  4:312202/1‑149 (MQ=255)                                                                                                       agcGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTc                                       <  8:11838/149‑1 (MQ=255)                                                                                                        gcGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGc                                                                                                           >  4:300794/1‑80 (MQ=255)                                                                                                        gcGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGc                                                                                                           <  3:300794/80‑1 (MQ=255)                                                                                                        gcGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCACGCCAGTGCTTTGTc                                       >  5:21073/1‑148 (MQ=255)                                                                                                          gggtgggtGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGac                                    <  6:166402/149‑1 (MQ=255)                                                                                                            gtgggtggCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAAcc                                                                    <  8:73368/115‑1 (MQ=255)                                                                                                            gtgggtggCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAAcc                                                                    >  7:73368/1‑115 (MQ=255)                                                                                                               ggtggCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCt                                                                                     >  4:5513/1‑95 (MQ=255)                                                                                                               ggtggCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCt                                                                                     <  3:5513/95‑1 (MQ=255)                                                                                                                 tggCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACTCTTGGCCCGCCAGTGCTTTGTCGACACCCTCg                             <  1:37688/149‑1 (MQ=255)                                                                                                                            ggCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCg                  >  1:157915/1‑149 (MQ=255)                                                                                                                              cATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGa                <  3:312202/149‑1 (MQ=255)                                                                                                                               aTTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGAc               <  4:118384/149‑1 (MQ=255)                                                                                                                                tttGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGgt                          <  6:177755/137‑1 (MQ=255)                                                                                                                                tttGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACt              <  7:168216/149‑1 (MQ=255)                                                                                                                                tttGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACt              >  2:109080/1‑149 (MQ=255)                                                                                                                                tttGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCGTTGTCGACACCCTCGGgt                          >  5:177755/1‑137 (MQ=255)                                                                                                                                     acaacCACCAGGCTGCGC‑GCCTTGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCTCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCt          <  7:35774/148‑1 (MQ=255)                                                                                                                                       aacCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTcc        >  2:77639/1‑148 (MQ=255)                                                                                                                                        tgcaccaGGC‑GCGCAGCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGAcc                    >  4:245184/3‑135 (MQ=255)                                                                                                                                        tgcaccaGGC‑GCGCAGCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGAcc                    <  3:245184/133‑1 (MQ=255)                                                                                                                                        tgcaccaGGC‑GCGCAGCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCt          >  7:268934/3‑145 (MQ=255)                                                                                                                                        tgcaccaGGC‑GCGCAGCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCt          <  8:268934/143‑1 (MQ=255)                                                                                                                                         ccaccaGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaacaa                                                                      <  3:25826/84‑1 (MQ=255)                                                                                                                                         ccaccaGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaacaa                                                                      >  4:25826/1‑84 (MQ=255)                                                                                                                                            ccaGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTgg  <  3:60016/149‑1 (MQ=255)                                                                                                                                            ccaGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTgg  <  8:43100/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CGCTGTTGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAAGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTG‑C‑ACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGG  >  NC_002947/1417183‑1417470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑