Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,647,572 Δ1 bp 100% coding (1150/1335 nt) oprB‑II ← carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,647,5720C.100.0% 152.2 / NA 34coding (1150/1335 nt)oprB‑IIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/19);  total (15/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTG  >  NC_002947/1647428‑1647707
                                                                                                                                                |                                                                                                                                       
cATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATaga                                                                                                                                    <  4:279942/149‑1 (MQ=255)
 aTCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATagag                                                                                                                                   >  1:125315/1‑149 (MQ=255)
    gACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACTGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGcc                                                                                                                                <  2:317878/149‑1 (MQ=255)
            acacCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCt                                                                                                                        >  8:301964/1‑149 (MQ=255)
            acacCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCt                                                                                                                        <  2:139707/149‑1 (MQ=255)
                  cgcccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGa                                                                                                                  <  7:64097/149‑1 (MQ=255)
                                   tACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTg                                                                                                 <  6:155785/149‑1 (MQ=255)
                                           gTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAgg                                                                                          >  4:80673/1‑148 (MQ=255)
                                           gTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGc                                                                                         <  7:301964/149‑1 (MQ=255)
                                                    tACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGAc                                                                                >  3:144148/1‑149 (MQ=255)
                                                    tACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGAc                                                                                <  6:275088/149‑1 (MQ=255)
                                                      ccGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGGTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAggc                                                                                                            <  7:178192/119‑1 (MQ=255)
                                                      ccGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAggc                                                                                                            >  8:178192/1‑119 (MQ=255)
                                                        gTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGc                                                                            <  5:332935/149‑1 (MQ=255)
                                                         tGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGAc                                                                                                                 >  4:73581/1‑111 (MQ=255)
                                                                gTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGc                                                                    <  2:125315/149‑1 (MQ=255)
                                                                   ggCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAg                                                                 <  5:46698/149‑1 (MQ=255)
                                                                     ccAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAggg                                                               <  3:80673/149‑1 (MQ=255)
                                                                         gTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGa                                                            >  5:235596/1‑148 (MQ=255)
                                                                               gCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGAt                                                     >  3:98968/1‑149 (MQ=255)
                                                                                          gcgcGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTgg                                          <  6:269538/146‑1 (MQ=255)
                                                                                           cTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGc                                                              <  1:142824/128‑1 (MQ=255)
                                                                                           cTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGc                                                              >  2:142824/1‑128 (MQ=255)
                                                                                           cTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGgc                                         <  3:40490/149‑1 (MQ=255)
                                                                                              ggCTCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAg                                                                                           <  5:112741/96‑1 (MQ=255)
                                                                                              ggCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAg                                                                                           >  6:112741/1‑96 (MQ=255)
                                                                                              ggCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGcgcg                                      <  4:98968/149‑1 (MQ=255)
                                                                                                     tACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCg                               >  4:64728/1‑149 (MQ=255)
                                                                                                     tACTCGGTGTCCTCCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATc                                >  1:81934/1‑148 (MQ=255)
                                                                                                                cTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGcgcg                                      <  6:68369/131‑1 (MQ=255)
                                                                                                                cTGCACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCttt                    >  4:250295/1‑149 (MQ=255)
                                                                                                                cTGCACTGGCAGGAAGCCCGGGTTGACGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCTATAGGCAGGGTTGACGTGCAGGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGcacgtt                    >  4:69133/1‑144 (MQ=255)
                                                                                                                   cACTGGCAGGAAGCCCGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTg                   <  4:144148/147‑1 (MQ=255)
                                                                                                                                  cGGGTTGTCGTAGT‑ATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgcctg  >  4:218464/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                       
CATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTG  >  NC_002947/1647428‑1647707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑