| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,218,365 | Δ1 bp | 12.8% | intergenic (‑191/+49) | PP_1958 ← / ← PP_5495 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,218,365 | 0 | C | . | 12.8% | 99.7 / 11.4 | 39 | intergenic (‑191/+49) | PP_1958/PP_5495 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (16/16); new base . (3/2); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01 | |||||||||||
TATTGAGCGCTCTGGTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCGAAGAAGGCTCCGAAGTCTCGA > NC_002947/2218218‑2218510 | tATTGAGCGCTCTGGTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCg < 6:157900/149‑1 (MQ=255) gcgcTCTGGTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTtctc < 1:175874/149‑1 (MQ=255) gcgcTCTGGTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTtctc < 3:167953/149‑1 (MQ=255) gcTCTGGTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCa < 7:71839/149‑1 (MQ=255) tGGTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCa > 3:247677/1‑148 (MQ=255) ggTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCAt < 4:247677/148‑1 (MQ=255) ggggATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGAcc > 3:46359/1‑148 (MQ=255) ggAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTacac < 4:46359/149‑1 (MQ=255) gaagTTCGCCTAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCATGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAgg < 5:229829/149‑1 (MQ=255) aagTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGAACCGCCATGG‑GCTACTCCAGCAtt < 2:247659/124‑2 (MQ=38) aagTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGAACCGCCATGG‑GCTACTCCAGCAtt < 2:243424/124‑2 (MQ=38) aagTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGAACCGCCATGG‑GCTACTCCAGCAtt > 1:243424/1‑123 (MQ=38) aagTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGAACCGCCATGG‑GCTACTCCAGCAtt > 1:247659/1‑123 (MQ=38) ttCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCATTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTgg > 4:62508/1‑149 (MQ=255) cAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAAc > 5:187549/1‑148 (MQ=255) cAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACg < 6:187549/149‑1 (MQ=255) gTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTAtt > 2:289838/1‑149 (MQ=255) cGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCCTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTAttt < 3:62508/149‑1 (MQ=255) cTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATa < 8:18824/87‑1 (MQ=255) cTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCACTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATa > 7:18824/1‑87 (MQ=255) aCTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATtcctc < 5:48046/149‑1 (MQ=255) tCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCg > 4:171106/1‑149 (MQ=255) gggTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCG‑GGATTGAAATtcctcc > 7:137252/1‑145 (MQ=255) gggTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCG‑GGATTGAAATtcctcc < 8:137252/145‑1 (MQ=255) gTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCAcgcg > 2:64658/1‑148 (MQ=255) aCGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTc > 1:232523/1‑149 (MQ=255) aGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGa < 1:64658/149‑1 (MQ=255) tttCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGGGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGt > 7:302921/1‑148 (MQ=255) ccccTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGacac > 5:74546/1‑133 (MQ=255) ccccTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGacac < 6:74546/133‑1 (MQ=255) ccccTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAg < 1:289838/147‑1 (MQ=255) ccccTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGc > 4:178063/1‑148 (MQ=255) ccTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTAt > 4:120646/1‑149 (MQ=255) tcgtaTGCTGGAGAACCGCCATGG‑GCTACTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCg > 4:98589/5‑148 (MQ=38) gCAATGCTGGAGTAGC‑CCATGGTGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCgaa < 8:302921/149‑1 (MQ=255) gAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCGAAGAAGGCTcc > 5:180806/1‑149 (MQ=255) gAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCGAAGAAGGCTc > 7:198548/1‑148 (MQ=255) c‑ccATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCGAAGAAGGCTCCGAAGtc > 4:191959/1‑149 (MQ=255) tGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCGAAGAAGGCTCCGAAGTCTCGa < 3:178063/149‑1 (MQ=255) | TATTGAGCGCTCTGGTAGCGGGGATGGAGGCGCGGAAGAAGTTCGCCAAGAGGTACCGGTACGTGACAGTAATTTCTACGACTCGGGTATAGGTCTTACGAGCATCAGGTTGCTTTCTGCCCCTCGCAATGCTGGAGTAGC‑CCATGGCGGTTCTCCAGCATACGACCGAAATCCATCTACACCAGGCTGGTAACGCTTATTGGCGTATTTGCGAGGATTGAAATTCCTCCGCTTCACGCGTAATTCCGACACACTCGGAGTCAAAGCTATCGAAGAAGGCTCCGAAGTCTCGA > NC_002947/2218218‑2218510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |