Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,755,091 Δ1 bp 8.5% coding (1417/3147 nt) czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,755,0910G. 8.5% 142.2 / 6.9 47coding (1417/3147 nt)czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (19/23);  new base . (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

AGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGG  >  NC_002947/2754951‑2755238
                                                                                                                                             |                                                                                                                                                    
agcGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGC‑aaa                                                                                                                                             <  5:266920/149‑2 (MQ=25)
   gcCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGC‑aaagat                                                                                                                                          <  5:50481/149‑5 (MQ=25)
   gcCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGC‑a                                                                                                                                               >  6:50481/1‑144 (MQ=25)
        ggTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTTGAGGGC‑AAGATGTTCCa                                                                                                                                     <  8:156250/149‑1 (MQ=255)
        ggTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCa                                                                                                                                     >  2:34723/1‑149 (MQ=255)
         gTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCAc                                                                                                                                    <  2:269938/149‑1 (MQ=255)
            gCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCAccc                                                                                                                                  >  4:83028/1‑148 (MQ=255)
             cATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTa                                                                                                                                >  3:183738/1‑149 (MQ=255)
                  tGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGcc                                                                                                                           >  5:12000/1‑149 (MQ=255)
                    aCCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCtt                                                                                                                         >  4:55599/1‑149 (MQ=255)
                      ccGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTa                                                                                                                       >  8:182117/1‑149 (MQ=255)
                         cggcgAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCg                                                                                                                    <  3:55599/149‑1 (MQ=255)
                             gAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAggg                                                                                                                                                  >  6:49877/1‑116 (MQ=255)
                             gAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAggg                                                                                                                                                  <  5:49877/116‑1 (MQ=255)
                              aaCGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTc                                                                                                               <  3:207849/149‑1 (MQ=255)
                                cGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCAt                                                                                                             >  4:92981/1‑149 (MQ=255)
                                  gTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATgg                                                                                                           <  7:182117/149‑1 (MQ=255)
                                   ttCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATg                                                                                                                                          <  7:50144/117‑1 (MQ=255)
                                   ttCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATg                                                                                                                                          >  8:50144/1‑117 (MQ=255)
                                   ttCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGc                                                                                                          >  8:76899/1‑149 (MQ=255)
                                       aTGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTg                                                                                                      <  5:261288/149‑1 (MQ=255)
                                          aGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTg                                                                                                   <  2:30887/149‑1 (MQ=255)
                                               tttGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgcgc                                                                                              >  3:301847/1‑149 (MQ=255)
                                               tttGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgcgc                                                                                              <  7:76899/149‑1 (MQ=255)
                                                   ccGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatg                                                                                          <  5:168379/149‑1 (MQ=255)
                                                    cGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatga                                                                                         <  3:83028/149‑1 (MQ=255)
                                                      cTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATc                                                                                       <  4:183738/149‑1 (MQ=255)
                                                      cTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATc                                                                                       <  3:92981/149‑1 (MQ=255)
                                                            gcgAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCg                                                                                 <  2:79607/149‑1 (MQ=255)
                                                             cgAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCgg                                                                                >  2:40187/1‑149 (MQ=255)
                                                             cgAAGCACGCCGACCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCgg                                                                                >  1:175547/1‑149 (MQ=255)
                                                                                     agggCAGTTGATCATCATGGTCGACTACCAGCCGAACTTTGACCT‑GACCGGGGTCAAGGCC‑AAGATGTTCCACACTATCGCCTTTACCGTGGTCATGGCCCAGTTGGGCTCTAAGATCCTTACGGTCACTTTCATACCGGCAGCCAtt                                                         >  8:122201/2‑148 (MQ=255)
                                                                                          aGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTg                                                   >  1:190769/1‑149 (MQ=255)
                                                                                               atcatcATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACgttcgttc                                                                    <  8:178626/127‑1 (MQ=255)
                                                                                               atcatcATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACgttcgttc                                                                    >  7:178626/1‑127 (MQ=255)
                                                                                                  atcatGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCAc                                           <  2:190769/149‑1 (MQ=255)
                                                                                                   tcatGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCAcc                                          >  1:180873/1‑149 (MQ=255)
                                                                                                        gtggttTACCTGCCTATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCaa                                     <  7:122201/149‑1 (MQ=255)
                                                                                                          ggtgTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAgg                                   <  2:180873/149‑1 (MQ=255)
                                                                                                              tACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCaa                               <  1:34723/149‑1 (MQ=255)
                                                                                                                 cTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAagg                             >  6:260126/1‑148 (MQ=255)
                                                                                                                     cGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGcc                                                      >  2:272133/1‑119 (MQ=255)
                                                                                                                        atcttGCCCTCGACCCCGGTC‑AGGGCAAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagagg                       >  4:260392/4‑148 (MQ=255)
                                                                                                                         cTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGccc                                                     >  6:297675/1‑116 (MQ=255)
                                                                                                                         cTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGccc                                                     <  5:297675/116‑1 (MQ=255)
                                                                                                                            tGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAgg                   <  6:12000/147‑1 (MQ=255)
                                                                                                                                      cGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGc        <  4:303651/149‑1 (MQ=255)
                                                                                                                                             gAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCAAGTTCGTTCCGGCAGCCATTGCACTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTaagg  >  3:261055/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
AGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGG  >  NC_002947/2754951‑2755238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑