Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,142,694:1 +A 7.5% coding (1029/1173 nt) PP_4526 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,142,6941.A 7.5% 202.0 / 11.7 53coding (1029/1173 nt)PP_4526MFS transporter
Reads supporting (aligned to +/- strand):  ref base . (20/29);  new base A (2/2);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GGTGTTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCATCCTGA  >  NC_002947/5142548‑5142838
                                                                                                                                                   |                                                                                                                                                 
ggTGTTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGTTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTg‑gc                                                                                                                                                 <  4:76592/149‑1 (MQ=255)
ggTGTTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTg‑gc                                                                                                                                                 >  8:220161/1‑149 (MQ=255)
  tgtTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTg‑gcgg                                                                                                                                               >  6:152148/1‑149 (MQ=255)
    ttGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTg‑gcggc                                                                                                                                              <  2:150322/148‑1 (MQ=255)
    ttGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑gcggcg                                                                                                                                             <  4:265753/149‑1 (MQ=255)
    ttGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑gcggcg                                                                                                                                             <  4:270243/149‑1 (MQ=255)
      gcgcTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCt                                                                                                                                           >  7:117029/1‑149 (MQ=255)
       cgcTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCt                                                                                                                                           >  7:267002/1‑148 (MQ=255)
        gcTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTca                                                                                                                                         <  2:293396/149‑1 (MQ=255)
         cTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTcag                                                                                                                                        <  5:248438/149‑1 (MQ=255)
           gtgtgGCGGGCCGTTCCTGGCCTGCAGGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGTCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTcagca                                                                                                                                      <  1:205473/149‑1 (MQ=255)
            tgtgGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTcagca                                                                                                                                      <  2:245898/148‑1 (MQ=255)
            tgtgGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTcagca                                                                                                                                      <  8:39729/148‑1 (MQ=255)
             gtgGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGC‑Cagcagc                                                                                                                                    >  5:249787/1‑149 (MQ=255)
                gCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGC‑Cagcagcgg                                                                                                                                  <  6:249787/148‑3 (MQ=255)
                     ccGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAg                                                                                                                            >  5:218147/1‑149 (MQ=255)
                     ccGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAg                                                                                                                            >  3:219464/1‑149 (MQ=255)
                      cGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGC‑Cagcagcgg                                                                                                                                  >  3:62861/1‑140 (MQ=255)
                      cGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTGAGCGCCGC‑Cagcagcgg                                                                                                                                  <  4:62861/142‑3 (MQ=255)
                      cGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGc                                                                                                                           <  8:117029/149‑1 (MQ=255)
                               ccTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTg                                                                                                                 <  4:147188/149‑1 (MQ=255)
                                cTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTg                                                                                                                 >  2:307050/1‑148 (MQ=255)
                                        aaCTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTg                                                                                                                 >  5:217366/1‑140 (MQ=255)
                                        aaCTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTg                                                                                                                 <  6:217366/140‑1 (MQ=255)
                                         aCTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCAt                                                                                                       >  4:97890/1‑149 (MQ=255)
                                          cTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCAtt                                                                                                      <  3:245963/149‑1 (MQ=255)
                                           tGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCAtt                                                                                                      >  8:77232/1‑148 (MQ=255)
                                             gTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTg                                                                                                     <  1:199862/147‑1 (MQ=255)
                                             gTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTg                                                                                                     <  8:267002/147‑1 (MQ=255)
                                             gTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGAt                                                                                                   >  7:115335/1‑149 (MQ=255)
                                                           gcgcgAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCggcgggcg                                                                                     >  6:61248/1‑149 (MQ=255)
                                                                 gTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtgg                                                                               <  2:43499/149‑1 (MQ=255)
                                                                    ggCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCAGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                            >  2:262564/1‑149 (MQ=255)
                                                                     gcgcCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCGCCTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                            <  1:228784/149‑1 (MQ=255)
                                                                     gcgcCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                            <  4:305824/148‑1 (MQ=255)
                                                                     gcgcCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtgg                                                                            <  6:218147/148‑1 (MQ=255)
                                                                      cgcCTCAGGCCACACAGCGCAATGCCGGGCTGCTGCCCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtggtg                                                                          <  7:220161/149‑1 (MQ=255)
                                                                         cacaGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGtggct                                                                       <  7:77232/149‑1 (MQ=255)
                                                                               ccACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCggg                                                                 >  8:152554/1‑149 (MQ=255)
                                                                                  cacaGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTtggtggt                                                                           >  7:108489/1‑136 (MQ=255)
                                                                                       cgcAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCa                                                         <  8:115335/149‑1 (MQ=255)
                                                                                        gcAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAg                                                        <  3:290870/149‑1 (MQ=255)
                                                                                         cAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGt                                                       >  4:2508/1‑149 (MQ=255)
                                                                                             gCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGcc                                                   >  6:155731/1‑149 (MQ=255)
                                                                                                   ctgctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGa                                             >  3:101223/1‑149 (MQ=255)
                                                                                                     gctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATg                                           >  3:153645/1‑149 (MQ=255)
                                                                                                     gctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATg                                           >  8:216118/1‑149 (MQ=255)
                                                                                                      ctgACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATg                                           <  1:307050/148‑1 (MQ=255)
                                                                                                         aCCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGcac                                        <  5:61248/148‑1 (MQ=255)
                                                                                                          ccGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGcgggcggg                                                                                   >  1:250715/1‑104 (MQ=255)
                                                                                                          ccGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGcgggcggg                                                                                   <  2:250715/104‑1 (MQ=255)
                                                                                                            gCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCacaaca                                    <  5:152148/149‑1 (MQ=255)
                                                                                                                      ccTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAgcgc                           <  5:155731/148‑1 (MQ=255)
                                                                                                                          ggggCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGGGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGt                       >  1:101343/1‑148 (MQ=255)
                                                                                                                          ggggCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGt                       <  4:101223/148‑1 (MQ=255)
                                                                                                                                   gAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAg              <  7:152554/148‑1 (MQ=255)
                                                                                                                                    aGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCT‑GTTGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGTGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGTGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGt            <  3:122124/149‑1 (MQ=255)
                                                                                                                                      tGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCCCATTGATGCTGGCGGCGGGCGGGTTGGGGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGtt           <  1:262564/148‑1 (MQ=255)
                                                                                                                                        ggCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTGCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCa        <  3:97890/149‑1 (MQ=255)
                                                                                                                                              tgctg‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCATCCTGa  <  3:2508/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGTGTTGCGCTGTGTGGCGGGCCGTTCCTGGCCTGCATGCAACTGGTCATGCAACGCTCGCGCGAGTTGGCGCCACAGGCCACACAGCGCAATGCCGGGCTGCTGACCGCATGCTTTGCCTTGGGGCAGTTGAGTGGGCCGCTGCTG‑GCGGCGCTCAGCAGCCACTACAGCG‑GTGGGCTGCAACCCGCATTGATGCTGGCGGCGGGCGGGTTGGTGGTGGCTGGCGGGTTGGTTCAGTTGCCGGGTGATGCACAACAAGGAAGCGCCTGTCAGGCCAAGGTTGCATCCTGA  >  NC_002947/5142548‑5142838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑