| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,032,795:1 | +G | 6.8% | intergenic (+59/+77) | dppA‑IV → / ← PP_5284 | dipeptide transport protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,032,795 | 1 | . | G | 6.8% | 236.6 / 11.3 | 59 | intergenic (+59/+77) | dppA‑IV/PP_5284 | dipeptide transport protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (23/32); new base G (2/2); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-02 | |||||||||||
GCGGCGACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAATGATGATGTGATC > NC_002947/6032647‑6032937 | gcggcgACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCa < 1:190407/149‑1 (MQ=255) gcgACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑Gtt < 3:225344/149‑1 (MQ=255) gcgACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑Gtt > 2:240042/1‑149 (MQ=255) cgACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCCGCCTGGCA‑GTTg > 1:22463/1‑149 (MQ=255) gACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGa < 6:185073/149‑1 (MQ=255) ccTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACg < 5:177805/149‑1 (MQ=255) cTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGc > 3:261272/1‑149 (MQ=255) gctgcGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTa < 1:193231/148‑1 (MQ=255) gctgcGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTa < 8:134889/148‑1 (MQ=255) ccAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGTCA‑ACTGCCAGGCTGGCGCTACCCCTGt < 8:151173/149‑1 (MQ=25) ccAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGt < 3:81415/149‑1 (MQ=255) cAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTa < 1:240042/149‑1 (MQ=255) aaGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAg < 6:250270/149‑1 (MQ=255) aaGGCGGCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAg < 7:67045/149‑1 (MQ=255) gTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCAAGCCCGGTAGGAGCg > 1:110626/1‑149 (MQ=255) tCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCgg < 1:263321/149‑1 (MQ=255) ggggTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGtt > 7:225807/1‑148 (MQ=255) gTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCGGTAGGAGCGGGGTTAcc > 8:34786/1‑149 (MQ=255) gTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCCCTCCTAAAGGGGTAGCGCCAGCCTGGGA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTaac > 8:63018/1‑147 (MQ=255) ccAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCcgc < 7:34786/149‑1 (MQ=255) ggTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGCCGCTACCCCTGTAGGAGCGGGTTTACCCGCGa > 1:52560/1‑148 (MQ=255) gcgcgCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCACGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGaaa < 8:290320/145‑1 (MQ=255) gTGACCCCGTTCGGGCGGCTGTATTTCAGCACGGTGACGGTGACCAAGTCAGCCTGTCCCGGCGTCTTCGCGGGTAACCCCGCTCCTACCGGGGTCGCGCCAGCCTGGCT‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGaaa < 2:256161/149‑1 (MQ=37) ggcggcTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGTCCCGTACTGa > 5:172717/1‑149 (MQ=255) ggggtgtaTTTCAGAATGTTTACGTTTACCATTTAAGCCTGTCCGGCCCTCTTCGCGGGTAAACCGGCTCCTACCGGGTTAGCGCCAGCTTGGAT‑GTTGACGCTACCCGTGTAGTAGGGGGTTTACCCGCGAAAGGGCCCGTACTGAcc < 6:172717/142‑1 (MQ=19) cTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAACGGCCCGTACTGACCCt > 7:290834/1‑148 (MQ=255) tGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGTTTTACCCGCGAAAGGGCCCGTACTGACCCtat < 4:261272/149‑1 (MQ=255) gATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAAAGGGGTAGCGCCAGCCGGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGGAAggg > 7:172465/1‑131 (MQ=255) tttCAGCAAGGTGACGGTGACCAAGAAACCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCAACCGGGGAAGCGCCAGCCTGGCA‑GTTGCCGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAgg < 7:63018/149‑1 (MQ=38) aGCAAGGTGACGGTTACCAAGTACGCGTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTTGCGCCAGCCTGGCT‑GTTGACGCTACCCCTGTAGTAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGctct < 6:126406/149‑1 (MQ=38) atggtgacggtcacCAAGTACGCCTGTACGGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAg < 2:52560/147‑1 (MQ=255) gacggtgacCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTc > 1:50513/1‑149 (MQ=255) acCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAg < 2:237789/148‑1 (MQ=255) cAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCGTGGCA‑GTTAACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGAcc > 1:99802/1‑149 (MQ=255) cAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCGACCCCGGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGGACGGACCCTATAAGGCTCTCAGTACTACCACACAcc > 4:214318/1‑149 (MQ=37) aaGTATGCCTGTACCGGCCTCTTCGCGGTTAAACCCGCTCCTACCGGGGTAGCGCCAGCTTGGCT‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACca < 2:90771/149‑1 (MQ=38) aGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATc < 2:50513/149‑1 (MQ=255) cccTGTACCGCCCGCTTCGCTGTTAAACCCTATCCTACAGGGGTAGCCCCAGCCTTGCC‑GATGACGCTACCCCTTTCGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAACGCTCTCAGTACTCCCTCAGACCACAATcc < 4:214228/148‑1 (MQ=255) cTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGTGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCAc < 8:225807/149‑1 (MQ=255) tcccGGCCTCTTCGCGGCTAAACCCGCTCCTACCGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAg < 5:192907/92‑1 (MQ=255) tttCGCGGGTAAACCCGCTCCTACAGGGGTAGCGTCA‑ACTGCCAGGCTGGCGCTACCCCTGTAGGAGCGGGTTTACCCGCGCAGGGGCCCGTACTGACCCTATAAGGCTCTCAGGACTCCCTCAGACCACCATCCACCCATGCTCggc > 5:178820/2‑146 (MQ=14) cgGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGAc < 8:238297/93‑1 (MQ=255) cgGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGAc > 7:238297/1‑93 (MQ=255) gTAAACCCGCTCCTACAGGGGTAGCGCCAGCTTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACaa < 2:99802/149‑1 (MQ=255) ccGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTc > 5:329935/1‑149 (MQ=255) ccTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTTCCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGAcc < 5:270135/130‑1 (MQ=255) ccTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTTCCCCTGTAGGAGCGGCTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCAATGCTCGAcc > 6:270135/1‑130 (MQ=255) aCAGGGGTAGCGTCA‑ACTGCCAGGCTGGCGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTc < 7:177603/97‑1 (MQ=14) aCAGGGGTAGCGTCA‑ACTGCCAGGCTGGCGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTc > 8:177603/1‑97 (MQ=14) gggTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCCGACCACACTCCACCCATGCTCGACCATCGACAACGGCACCCCATCACCa > 5:235370/1‑147 (MQ=255) cgcCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGTCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAatgatgat > 3:234149/1‑149 (MQ=255) ccAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTcc < 1:190881/130‑1 (MQ=255) ccAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTcc > 2:190881/1‑130 (MQ=255) ccAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCAc < 6:241247/137‑1 (MQ=255) ccAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCAc > 5:241247/1‑137 (MQ=255) ccTGGCC‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAATGATGATGTGATc < 3:214318/149‑1 (MQ=255) ccTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAATGGCCCGTACTGACCCTATAAGGctc < 7:177863/72‑1 (MQ=255) ccTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAATGGCCCGTACTGACCCTATAAGGctc > 8:177863/1‑72 (MQ=255) tGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCa < 6:129333/130‑1 (MQ=255) tGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCa > 5:129333/1‑130 (MQ=255) | GCGGCGACCTTGCTGCGCCAAGGCGTCGAGGGGTACCAGGTGAGCCCGTTCGGGCGGCTGGATTTCAGCAAGGTGACGGTGACCAAGTAAGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGGGTAGCGCCAGCCTGGCA‑GTTGACGCTACCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCCGTACTGACCCTATAAGGCTCTCAGTACTCCCTCAGACCACAATCCACCCATGCTCGACCATCGACAACGGCTCCCCATCACCAATGATGATGTGATC > NC_002947/6032647‑6032937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |