| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 92.9% | 84.3 / 0.5 | 28 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (13/13); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126502‑1126788 | ggggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAA‑cccgc < 7:4197/149‑4 (MQ=255) aGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑aaa > 2:84130/1‑122 (MQ=255) aGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCCCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑aaa < 1:84130/122‑1 (MQ=255) tCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGCTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGACGTGATGGCACGCACCCAATAGC‑CGGTAAAA‑CCGCTCCAACACAAAGCCCCCCCCCCaa > 8:195124/1‑148 (MQ=255) cGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGc < 5:270772/149‑1 (MQ=255) gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTATAGCCACCAGAGCAATAGC‑GGGT‑AAACCCGCGCCAACACAGAGCCCGcccccccaccc > 6:270772/1‑144 (MQ=255) ggACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCCCGAATGCGGAGGTATAGCCACCCTCGCATTAGC‑GGGT‑AAACCCGCGACTACACAGAGCCCCCCCCCCAACCCAG‑GGGGCGGGGGGGGCTAt > 6:308547/1‑148 (MQ=255) gACCTCCTGCCAGGATGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTAACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATc < 5:92212/148‑1 (MQ=255) cctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACagag < 7:84434/111‑1 (MQ=255) ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAAAGCGGAGGTATAGCCACCAACGCAATAGCGGGGA‑AAA‑CCCCGCCGAAACAGAGCCCCCCACCCAAGCCAG‑GTGGCGGGGCGGGCTATCAAGCGTCCt > 2:92585/1‑148 (MQ=255) ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGTGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGTCGGGGAg < 1:8997/132‑1 (MQ=255) ttgGGGGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctct < 4:214911/149‑1 (MQ=255) aTTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACgg < 4:85829/149‑1 (MQ=255) ggCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGCGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCacac < 1:92585/149‑1 (MQ=255) gttttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGTT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg < 7:236713/147‑1 (MQ=255) ggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg > 2:300509/1‑149 (MQ=255) cccGCGAATGCGGAGGTATAGCCACCAGCGCTTTCGG‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGACGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg < 1:142335/149‑1 (MQ=255) cgcgAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGCT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 8:23387/1‑149 (MQ=255) ggAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 3:233633/1‑149 (MQ=255) gTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAggg > 7:29157/1‑76 (MQ=255) gTATAGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAggg < 8:29157/76‑1 (MQ=255) ataGCCACCATCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc > 8:171255/1‑149 (MQ=255) ccaccaTCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 7:155828/1‑149 (MQ=255) ccaccaTCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 2:274323/1‑149 (MQ=255) caccaTCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 7:46289/1‑149 (MQ=255) accaTCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGcc < 1:274323/149‑1 (MQ=255) caTCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa < 1:300509/149‑1 (MQ=255) aTCGCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATGGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc > 3:110067/1‑149 (MQ=255) aTCCCATTCGC‑GGGT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc < 5:36442/149‑1 (MQ=255) c‑gGGT‑AAACCCGATCATACACAGAGCCCGCCACCCAACCCAG‑GTGGCGGCGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGCCCGGCGGCCAg > 2:312922/1‑149 (MQ=255) gT‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc > 7:243525/1‑149 (MQ=255) | GGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGT‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAG‑GTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126502‑1126788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |