Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	278,742:1	+A	100%	intergenic (‑237/+77)	PP_0224 ← / ← sctC	DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	278,742	1	.	A	100.0%	162.6 / NA	49	intergenic (‑237/+77)	PP_0224/sctC	DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (25/24);  total (25/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGC  >  NC_002947/278595‑278884                                                                                                                                                     |                                                                                                                                                aTGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGt                                                                                                                                                  <  8:43994/148‑1 (MQ=255)   gTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCCAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑aaa                                                                                                                                              >  1:53435/1‑148 (MQ=255)          cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTc                                                                                                                                        <  4:309744/149‑1 (MQ=255)          cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTc                                                                                                                                        <  1:174892/149‑1 (MQ=255)           aGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTcc                                                                                                                                       <  1:193023/149‑1 (MQ=255)              tGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTa                                                                                                                                     >  1:138061/1‑148 (MQ=255)                aTCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTa                                                                                                                                     <  5:330614/146‑1 (MQ=255)                      aCAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGc                                                                                                                            <  3:111761/149‑1 (MQ=255)                            gtgtGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTc                                                                                                                         >  3:77246/1‑146 (MQ=255)                            gtgtGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAg                                                                                                                      <  2:138061/149‑1 (MQ=255)                               tgCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATc                                                                                                                   >  7:246041/1‑149 (MQ=255)                                 cgggtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                                  <  8:186238/146‑1 (MQ=255)                                 cTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                                  <  4:77246/148‑1 (MQ=255)                                     tccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCa                                                                                                            <  8:246041/149‑1 (MQ=255)                                       cggtggtccggtATATATACCCCAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCAc                                                                                                           <  2:53435/148‑1 (MQ=255)                                          tggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTa                                                                                                       <  5:56269/149‑1 (MQ=255)                                                  tatatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCggg                                                                                               <  3:73293/149‑1 (MQ=255)                                                    tatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGtt                                                                                             <  2:256153/149‑1 (MQ=255)                                                        taCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTAcc                                                                                         <  2:299853/149‑1 (MQ=255)                                                         aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTg                                                                                                         <  6:320218/132‑1 (MQ=255)                                                         aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTg                                                                                                         >  5:320218/1‑132 (MQ=255)                                                           ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCcgc                                                                                      >  1:193090/1‑149 (MQ=255)                                                                tGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGt                                                                                              <  8:224913/136‑1 (MQ=255)                                                                tGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGt                                                                                              >  7:224913/1‑136 (MQ=255)                                                                 gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                                  <  4:169304/116‑1 (MQ=255)                                                                 gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                                  >  3:169304/1‑116 (MQ=255)                                                                  aTTTATAAAATTTTTATTAACTAACTTTTTGTAATATTTATAGAAGGGGTATTTGTTACAGAGTTGCCGGGGTCTTCGCGGTTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTTGTAGCGGTTTTACCCGCGAAAgg                                                                                <  6:122375/148‑1 (MQ=255)                                                                        aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAAACCGCTGCTACACACATGCCACAAGCAGTG‑TGCACTGTAGGAGCGGGTTTACCCTCGAAAGGGCCggg                                                                          >  8:194522/1‑147 (MQ=255)                                                                                   gAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTc                                                              >  5:184803/1‑149 (MQ=255)                                                                                     actaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAAACCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCg                                                             >  7:296993/1‑148 (MQ=255)                                                                                      ctaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCg                                                             >  5:319352/1‑147 (MQ=255)                                                                                         actTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGCGGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAgg                                                         <  1:88671/149‑1 (MQ=39)                                                                                               tGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACTCACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGt                                                  >  4:294193/1‑149 (MQ=255)                                                                                               tGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGt                                                  >  2:57924/1‑149 (MQ=255)                                                                                                 gAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTcc                                                >  6:102859/1‑149 (MQ=255)                                                                                                    tatTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCCCTCCTAACCACAATCCAAAAAATCTG‑TGCAATGTAGGAGCGGGTTTTACCGCGAAAGGGCCCGAACAGGTTTACACGGAGGCCGCAGTccc                                               >  7:172745/1‑147 (MQ=255)                                                                                                     atTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAg                                            <  2:193090/149‑1 (MQ=255)                                                                                                             aaGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc                                    >  7:115291/1‑149 (MQ=255)                                                                                                               gggggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCCCTCCAAAACACAAGCCGCAAGATATG‑TGCCCAGTTGGAGCGGGTTTAACCGCGCAAGGGCCGGAACACGGTTAATAGGAGGCCGCAGTCCCCAGGAACGTgcg                                   >  4:301250/1‑148 (MQ=255)                                                                                                                ggggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCcgc                                                                                      <  3:120158/96‑1 (MQ=255)                                                                                                                ggggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCcgc                                                                                      >  4:120158/1‑96 (MQ=255)                                                                                                                 gggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACTTGCCTCATGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCaa                                <  6:253116/149‑1 (MQ=255)                                                                                                                     tttGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATTCCTCAAGATCTG‑TGCACTGTAGGAGCCGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCCCAGTCCCGAGGAACTTGCGCAAGAAc                            >  7:19753/1‑149 (MQ=255)                                                                                                                          tACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcg                       >  2:201638/1‑149 (MQ=255)                                                                                                                          tACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcg                       >  2:196954/1‑149 (MQ=255)                                                                                                                             agcggTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGt                    <  8:298058/146‑1 (MQ=255)                                                                                                                                  tGCCGGGCTCTTCGCGGGTAAACCCGCAGCAACACACATGCCTCAAGAAATG‑TGCCCAGTAGGACCCGGTTTACCCCCGAAAGGGCAGGAACACGTTTACTACGAGGCCGAAGTGCCCAGGAACTTGCGCAACAACTGCCCCGAACGct               >  2:248784/1‑149 (MQ=255)                                                                                                                                    ccGGGCTCTTCGCGGGTAAACCCGCTCCTACACCCATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctct             >  7:294885/1‑149 (MQ=255)                                                                                                                                              cgcgGGTAAACCCGCTCCTACTCACATGCCTCAATATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCgg     <  3:294193/147‑1 (MQ=255)                                                                                                                                              cgcgGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgcg   >  7:34903/1‑149 (MQ=255)                                                                                                                                               gcgGGTAAACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGgcgc  >  6:165276/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                ATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTG‑TGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGC  >  NC_002947/278595‑278884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑