Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 307,865:1 +C 100% intergenic (+880/‑989) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947307,8651.C98.4% 204.8 / ‑3.3 61intergenic (+880/‑989)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (31/29);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307718‑308008
                                                                                                                                                     |                                                                                                                                               
aaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑c                                                                                                                                                >  5:294893/1‑149 (MQ=255)
   ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGTCAGCCGAAAG‑GGta                                                                                                                                             <  6:138934/149‑2 (MQ=255)
      tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAc                                                                                                                                           <  6:268501/149‑1 (MQ=255)
      tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAc                                                                                                                                           >  8:132052/1‑149 (MQ=255)
                 cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGCCCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTga                                                                                                                                >  7:142397/1‑149 (MQ=255)
                     ccTTCCTGCTGCCGGCTGCCGATGTACTGCCTCTCCACCGCGCGGCCAACATCGGCGAAGAACGCGATGTGACCTTGTTC‑TTCCGCCTGTCCGCCACGGGCAAGACTCCCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGCgacgac                                                                                                                            <  4:136376/148‑1 (MQ=255)
                     aCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTgacgac                                                                                                                            <  3:30932/149‑1 (MQ=255)
                              tgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGt                                                                                                                   >  8:32188/1‑149 (MQ=255)
                                   gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTTACGACGAGCACGGTTgggg                                                                                                              <  1:304352/149‑1 (MQ=255)
                                       ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGaa                                                                                                          <  7:132052/149‑1 (MQ=255)
                                         gATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAgg                                                                                                        >  7:207234/1‑149 (MQ=255)
                                          aTGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc                                                                                                       <  6:41599/149‑1 (MQ=255)
                                              aCTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTg                                                                                                   >  6:141357/1‑149 (MQ=255)
                                               cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCt                                                                                                                                         <  8:110363/110‑1 (MQ=255)
                                               cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCt                                                                                                                                         >  7:110363/1‑110 (MQ=255)
                                                 gCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                             >  7:187427/1‑100 (MQ=255)
                                                 gCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                             <  8:187427/103‑4 (MQ=255)
                                                  ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                                <  7:32188/148‑1 (MQ=255)
                                                    tATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCGTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                               >  1:76029/1‑148 (MQ=255)
                                                    tATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                              >  3:3587/1‑148 (MQ=255)
                                                      tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                            <  6:294893/148‑1 (MQ=255)
                                                         aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATg                                                                                        >  7:26755/1‑149 (MQ=255)
                                                             cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAg                                                                                    >  4:120054/1‑149 (MQ=255)
                                                                  ccAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACAGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                               >  3:205804/1‑149 (MQ=255)
                                                                   cAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct                                                                              >  3:132803/1‑149 (MQ=255)
                                                                   cAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct                                                                              >  8:168474/1‑149 (MQ=255)
                                                                    aaCATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgctg                                                                             >  7:196569/1‑149 (MQ=255)
                                                                         cGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTa                                                                          >  8:157752/1‑147 (MQ=255)
                                                                         cGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTa                                                                          <  7:157752/147‑1 (MQ=255)
                                                                          ggCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                       <  7:190279/149‑1 (MQ=255)
                                                                           gCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa                                                                                          <  6:273096/129‑1 (MQ=255)
                                                                           gCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa                                                                                          >  5:273096/1‑129 (MQ=255)
                                                                             gaagaaGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                                    <  8:207234/149‑1 (MQ=255)
                                                                               agaagGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                  >  6:308027/1‑149 (MQ=255)
                                                                                gaagGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTg                                                                 <  5:141357/149‑1 (MQ=255)
                                                                                       aTGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                          <  5:308027/149‑1 (MQ=255)
                                                                                       aTGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACACGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                          <  2:76029/149‑1 (MQ=255)
                                                                                            aCCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc                                                     >  5:286459/1‑149 (MQ=255)
                                                                                              cTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    >  5:49458/1‑148 (MQ=255)
                                                                                              cTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    >  2:58349/1‑148 (MQ=255)
                                                                                                 gTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAc                                                                                                                                           <  4:274963/58‑1 (MQ=255)
                                                                                                 gTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAc                                                                                                                                           >  3:274963/1‑58 (MQ=255)
                                                                                                 gTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgag                                                 <  4:3587/148‑1 (MQ=255)
                                                                                                        cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                           >  7:73965/1‑148 (MQ=255)
                                                                                                        cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                           >  7:69537/1‑148 (MQ=255)
                                                                                                            cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGcc                                      >  7:98749/1‑149 (MQ=255)
                                                                                                               tCCGGCACAGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                     <  4:205804/147‑1 (MQ=255)
                                                                                                                 cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCa                                 >  2:248316/1‑149 (MQ=255)
                                                                                                                   gCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATc                               <  8:69537/149‑1 (MQ=255)
                                                                                                                   gCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATc                               <  8:73965/149‑1 (MQ=255)
                                                                                                                     acgggCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg                             <  8:142397/146‑1 (MQ=255)
                                                                                                                      cTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTgg                            <  1:248316/149‑1 (MQ=255)
                                                                                                                           aaGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGc                       <  8:98749/149‑1 (MQ=255)
                                                                                                                             gACTACCCTTTCGGCTGACGAAAGCCGTTACCTGTTCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGcct                     <  6:301061/149‑2 (MQ=255)
                                                                                                                             gACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCa                     >  1:47514/1‑149 (MQ=255)
                                                                                                                              aCTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                    <  6:49458/149‑1 (MQ=255)
                                                                                                                              aCTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                    >  8:80858/1‑149 (MQ=255)
                                                                                                                                  cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa                                                                                     >  7:43849/1‑80 (MQ=255)
                                                                                                                                  cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa                                                                                     <  8:43849/80‑1 (MQ=255)
                                                                                                                                  cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTa                                                                          <  7:56007/91‑1 (MQ=255)
                                                                                                                                  cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTa                                                                          >  8:56007/1‑91 (MQ=255)
                                                                                                                                  cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg                <  2:47514/149‑1 (MQ=255)
                                                                                                                                            tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAGCATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATATGTAAAGCCATCAAGCACGGCGCAg      >  2:249077/1‑149 (MQ=255)
                                                                                                                                                gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt  >  3:223599/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTC‑TTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307718‑308008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑