| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,282,294:1 | +C | 12.1% | coding (72/300 nt) | PP_2883 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,282,294 | 1 | . | C | 12.1% | 108.0 / 12.4 | 33 | coding (72/300 nt) | PP_2883 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (15/14); new base C (2/2); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
AGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGCGGCAG > NC_002947/3282146‑3282442 | aGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAggtgg > 4:121760/1‑149 (MQ=255) aGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCA‑CCACCTTGAGGTGGCTGCACGcc < 8:226145/149‑1 (MQ=255) tCGGGGGCTTGGCAACGAACGCAATCCGCCAGGCCTGCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAg < 6:318850/149‑1 (MQ=255) aacgaacgCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCCCGCCAAGGGCCACATTGCGCAGGCACTGGTAGACa > 2:139854/1‑149 (MQ=255) aacgaacgCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACa > 6:241192/1‑149 (MQ=255) aaTGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCg > 8:163189/1‑149 (MQ=255) gcCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGtt > 6:260580/1‑146 (MQ=255) gcCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGtt < 5:260580/146‑1 (MQ=255) ccAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTgcgc < 7:34840/119‑1 (MQ=255) ccAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTgcgc > 8:34840/1‑119 (MQ=255) gCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAAc < 3:121760/149‑1 (MQ=255) cTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAg > 8:5750/1‑115 (MQ=255) gTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCtgtg < 7:163189/149‑1 (MQ=255) tATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTgg < 5:275461/149‑1 (MQ=255) cAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTc < 5:241192/148‑1 (MQ=255) gggCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTc > 2:62150/1‑146 (MQ=255) ccAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGAt > 3:295302/1‑148 (MQ=255) ccAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGAt > 5:275747/1‑148 (MQ=255) cAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGc > 5:208869/1‑149 (MQ=255) cTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGTAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGtt > 6:294322/1‑149 (MQ=255) gATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTg < 4:295302/147‑1 (MQ=255) tGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGgcgc < 6:275747/149‑1 (MQ=255) cGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtga > 1:51040/1‑149 (MQ=255) cGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtga > 5:220823/1‑149 (MQ=255) gACATCGATCAGGCCGCCATCGCGGCGTGTAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtgat < 5:294322/149‑1 (MQ=255) gATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGAtctc > 3:39104/1‑149 (MQ=255) aGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGc < 1:62150/148‑1 (MQ=255) gccATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATc < 1:139854/149‑1 (MQ=255) cggcgTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTAGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCg < 8:74127/149‑1 (MQ=255) ggcgTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGa < 2:51040/149‑1 (MQ=255) ggcgTGCA‑CCACCTTGAGGTGGCTGCACGCCAAGGGCCACATTGAGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCg > 6:38533/1‑148 (MQ=255) gcgTGCA‑CCACCTTGAGGTGGCTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGa < 5:38533/148‑1 (MQ=255) ccACCTTGAGGTGGCTGCACGCCGAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCtt > 4:309399/1‑148 (MQ=255) aCCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGaaa > 8:195421/1‑149 (MQ=255) g‑tgCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGCGGCAg > 8:102384/1‑149 (MQ=255) g‑tgCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTAAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGCGGCa < 4:39104/148‑1 (MQ=255) | AGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGCGGCAG > NC_002947/3282146‑3282442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |