New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 1520999	42 (0.830)	5 (0.100)	4/244	NT	11.1%	coding (256/1353 nt)	murD	UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
	?	NC_002947	= 1521021	40 (0.840)					coding (278/1353 nt)	murD	UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

GATACCCGCGAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCC  >  NC_002947/1520858‑1521145                                                                                                                                               |                                                                                                                                                   gATACCCGCGAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCtt                                                                                                                                             <  1:312546/149‑1 (MQ=255)  aTACCCGCGAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTg                                                                                                                                            <  5:269422/149‑1 (MQ=255)   tACCCGCGAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTg                                                                                                                                            >  3:139823/1‑148 (MQ=255)      ccGCGAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTg                                                                                                                                            >  1:191696/1‑145 (MQ=255)      ccGCGAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTg                                                                                                                                            <  2:191696/145‑1 (MQ=255)       cgcgAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTgcagca                                                                                                                                       <  8:158556/149‑1 (MQ=255)                     ggAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCgcggcagcctgctgcaaggccggcgtg                                                                                                                         <  3:154376‑M1/149‑28 (MQ=255)                     ggAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCt                                                                                                                                   >  6:293979/1‑139 (MQ=255)                     ggAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCt                                                                                                                                   <  5:293979/139‑1 (MQ=255)                          tGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCgcgtggcgt                                                                                                                     >  4:237921/1‑148 (MQ=255)                            gAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCgcgtggcgtg                                                                                                                    >  2:43657/1‑147 (MQ=255)                              aaCCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGc                                                                                                                >  7:198609/1‑149 (MQ=255)                                 ccTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCgcggcagcctgctgcaaggccggcgtggccaa                                                                                                                    <  8:178973‑M1/142‑33 (MQ=255)                                 ccTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCgcggcagcctgctgcaaggccggcgtggccaa                                                                                                                    >  7:178973‑M1/1‑110 (MQ=255)                                  cTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTc                                                                                                            <  4:139823/149‑1 (MQ=255)                                       ccGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCgcggcagcctgctgcaaggccggcgtg                                                                                                                         <  5:309016‑M1/131‑28 (MQ=255)                                       ccGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCgcggcagcctgctgcaaggccggcgtg                                                                                                                         >  6:309016‑M1/1‑104 (MQ=255)                                            aTTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATa                                                                                                  <  3:237921/149‑1 (MQ=255)                                               aCCCGCAGGTGGACGTGCGCTGTGGTGAACTGGACGGGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATaga                                                                                                <  8:63350/148‑1 (MQ=255)                                                cctgcgggTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGc                                                                                              >  8:53771/7‑149 (MQ=255)                                                              tGCGCTGTGGTGAACTGGACGTGGACTTCCTGGGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCAt                                                                                <  4:187003/149‑1 (MQ=255)                                                                 gcTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCTGGTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGc                                                                              >  1:71384/1‑149 (MQ=255)                                                                   tgtgGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGcc                                                                             >  7:79459/1‑147 (MQ=255)                                                                       gtgAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGgcg                                                                       >  6:42588/1‑149 (MQ=255)                                                                            cTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGAt                                                                  >  1:290569/1‑149 (MQ=255)                                                                                   tGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGcc                                                           <  5:42588/149‑1 (MQ=255)                                                                                   tGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGcc                                                           <  2:290569/149‑1 (MQ=255)                                                                                    ggACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCa                                                          >  2:263881/1‑149 (MQ=255)                                                                                          ccTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAg                                                      <  1:43657/147‑1 (MQ=255)                                                                                           cTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGTCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTgg                                                   >  4:96520/1‑149 (MQ=255)                                                                                             gtgCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGtt                                                 >  7:120267/1‑149 (MQ=255)                                                                                              tgCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTc                                                <  6:46321/149‑1 (MQ=255)                                                                                              tgCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTc                                                >  7:283246/1‑149 (MQ=255)                                                                                              tgCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTc                                                >  8:83765/1‑149 (MQ=255)                                                                                                  gcgcCAACGAGCT‑GTACGTGAGCCCCGGCCTGGTCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAAc                                            <  3:96520/149‑1 (MQ=255)                                                                                                      cAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGcc                                         >  4:261493/1‑148 (MQ=255)                                                                                                                    gTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCac                           >  3:285386/1‑149 (MQ=255)                                                                                                                     tGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCac                           >  2:285944/1‑148 (MQ=255)                                                                                                                      gAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCacga                         >  6:118685/1‑149 (MQ=255)                                                                                                                      gAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAACAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCacga                         >  1:52771/1‑149 (MQ=255)                                                                                                                         ccccGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGc                      <  8:283246/149‑1 (MQ=255)                                                                                                                          cccGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCt                     >  6:165614/1‑149 (MQ=255)                                                                                                                              gcgtggccaAGGCCAGGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTc                 >  3:262980/4‑149 (MQ=255)                                                                                                                              gcctggcctTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTc                 >  7:115164/1‑149 (MQ=255)                                                                                                                               cctggcctTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCg                >  7:100594/1‑149 (MQ=255)                                                                                                                                      ttGGCCACGCCGGCCTTGCAGCAGGCTGCCGCgcgtggccaaggccaggccggggctcacgtacagctcgttggcgcggcacaggaagtccacgtccagttcaccacagcgcacttccacctgcgggtaatcacggcgcagg                >  7:178973‑M2/1‑32 (MQ=255)                                                                                                                                      ttGGCCACGCCGGCCTTGCAGCAGGCTGCCGCgcgtggccaaggccaggccggggctcacgtacagctcgttggcgcggcacaggaagtccacgtccagttcaccacagcgcacttccacctgcgggtaatcacggcgcagg                <  8:178973‑M2/142‑111 (MQ=255)                                                                                                                                       aggCCAGGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATg        <  4:262980/148‑1 (MQ=255)                                                                                                                                          ccACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGgcc     <  2:71384/149‑1 (MQ=255)                                                                                                                                           cACGCCGGCCTTGCAGCAGGCTGCCGCgcgtggccaaggccaggccggggctcacgtacagctcgttggcgcggcacaggaagtccacgtccagttcaccacagcgcacttccacctgcgggtaatcacggcgcagggtttccagttcc    <  3:154376‑M2/149‑123 (MQ=255)                                                                                                                                           cACGCCGGCCTTGCAGCAGGCTGCCGCgcgtggccaaggccaggccggggctcacgtacagctcgttggcgcggcacaggaagtccacgtccagttcaccacagcgcacttccacctgcgggtaatcacgg                      >  6:309016‑M2/1‑27 (MQ=255)                                                                                                                                           cACGCCGGCCTTGCAGCAGGCTGCCGCgcgtggccaaggccaggccggggctcacgtacagctcgttggcgcggcacaggaagtccacgtccagttcaccacagcgcacttccacctgcgggtaatcacgg                      <  5:309016‑M2/131‑105 (MQ=255)                                                                                                                                              gCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGgccgcc  >  6:287902/1‑148 (MQ=255)                                                                                                                                              gCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGgccgcc  >  3:133669/1‑148 (MQ=255)                                                                                                                                              gCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGgccgcc  >  5:293262/1‑148 (MQ=255)                                                                                                                                              gCCGGCCTTGCAGCAGGCTGACGCGCGTGACGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCTCCGATAGTTGCCATCAGTCGATCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGgccgcc  >  1:188499/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                   GATACCCGCGAGCAGCCGCCGGAACTGGAAACCCTGCGCCGTGATTACCCGCAGGTGGAAGTGCGCTGTGGTGAACTGGACGTGGACTTCCTGTGCCGCGCCAACGAGCT‑GTACGTGAGCCCCGGCCTGGCCTTGGCCACGCCGGCCTTGCAGCAGGCTGCCGCGCGTGGCGTGAAGCTGTCTGGCGACATAGAGCTGTTTGCTCGCCATGCCAAGGCGCCGATCGTTGCCATCAGTGGTTCCAACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCC  >  NC_002947/1520858‑1521145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.